Antimicrobial Resistance Patterns and Dynamics of Extended-Spectrum β-Lactamase-Producing Uropathogenic <i>Escherichia coli</i> in Cusco, Peru
Urinary tract infections (UTIs) are a common human infection. Antibiotic resistance in extended-spectrum β-lactamase (ESBL)-producing uropathogenic <i>E. coli</i> (UPEC) is a major therapeutic challenge due to limited treatment alternatives. The aim was to characterize the antimicrobial...
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MDPI AG
2021-04-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/10/5/485 |
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| author | Steev Loyola Fátima Concha-Velasco Jimena Pino-Dueñas Nancy Vasquez-Luna Paola Juarez Carlos Llanos Guillermo Salvatierra Jesus Tamariz Andres G. Lescano |
| author_facet | Steev Loyola Fátima Concha-Velasco Jimena Pino-Dueñas Nancy Vasquez-Luna Paola Juarez Carlos Llanos Guillermo Salvatierra Jesus Tamariz Andres G. Lescano |
| author_sort | Steev Loyola |
| collection | DOAJ |
| description | Urinary tract infections (UTIs) are a common human infection. Antibiotic resistance in extended-spectrum β-lactamase (ESBL)-producing uropathogenic <i>E. coli</i> (UPEC) is a major therapeutic challenge due to limited treatment alternatives. The aim was to characterize the antimicrobial resistance (AMR) and dynamics of ESBL-producing UPEC isolates from UTI cases seen at a local hospital in Cusco, Peru. Ninety-nine isolates from respective patients were characterized against 18 different antibiotics. Latent class analysis (LCA) was used to evaluate the dynamics across the study time according to resistance patterns. The median age of patients was 51 years old, and nearly half were women. ESBL-producing UPEC isolates were slightly more frequent in outpatient services than emergency rooms, and there were higher resistance rates in males compared to females. Half of the ESBL producers were resistant to aminoglycosides and nitrofurantoin. Cefoxitin and fosfomycin resistance was 29.3% and 14.1%, respectively. Resistance to carbapenems was not observed. All isolates were multidrug-resistant bacteria, and 16.2% (16/99) were also classified as extensively drug-resistant bacteria. The resistance patterns varied across the study time and differed regarding sex and healthcare service. The study revealed high levels of AMR to commonly used antimicrobials and a dynamic circulation of ESBL-producing UPEC isolates with varying resistance patterns. |
| first_indexed | 2024-03-10T12:04:07Z |
| format | Article |
| id | doaj.art-c0422e6d5db44e78bda7f62b56bcf8a0 |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-10T12:04:07Z |
| publishDate | 2021-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-c0422e6d5db44e78bda7f62b56bcf8a02023-11-21T16:43:22ZengMDPI AGAntibiotics2079-63822021-04-0110548510.3390/antibiotics10050485Antimicrobial Resistance Patterns and Dynamics of Extended-Spectrum β-Lactamase-Producing Uropathogenic <i>Escherichia coli</i> in Cusco, PeruSteev Loyola0Fátima Concha-Velasco1Jimena Pino-Dueñas2Nancy Vasquez-Luna3Paola Juarez4Carlos Llanos5Guillermo Salvatierra6Jesus Tamariz7Andres G. Lescano8Laboratorio de Resistencia Antibiótica e Inmunopatología, Facultad de Medicina, Universidad Peruana Cayetano Heredia, Lima 15102, PeruDepartamento de Apoyo al Diagnóstico, Hospital Antonio Lorena, Cusco 08001, PeruDepartamento de Apoyo al Diagnóstico, Hospital Antonio Lorena, Cusco 08001, PeruDepartamento de Apoyo al Diagnóstico, Hospital Antonio Lorena, Cusco 08001, PeruLaboratorio de Resistencia Antibiótica e Inmunopatología, Facultad de Medicina, Universidad Peruana Cayetano Heredia, Lima 15102, PeruLaboratorio de Resistencia Antibiótica e Inmunopatología, Facultad de Medicina, Universidad Peruana Cayetano Heredia, Lima 15102, PeruLaboratorio de Genómica Microbiana, Departamento de Ciencias Celulares y Moleculares, Facultad de Ciencias y Filosofía, Universidad Peruana Cayetano Heredia, Lima 15102, PeruLaboratorio de Resistencia Antibiótica e Inmunopatología, Facultad de Medicina, Universidad Peruana Cayetano Heredia, Lima 15102, PeruEmerge, Emerging Diseases and Climate Change Research Unit, School of Public Health and Administration, Universidad Peruana Cayetano Heredia, Lima 15102, PeruUrinary tract infections (UTIs) are a common human infection. Antibiotic resistance in extended-spectrum β-lactamase (ESBL)-producing uropathogenic <i>E. coli</i> (UPEC) is a major therapeutic challenge due to limited treatment alternatives. The aim was to characterize the antimicrobial resistance (AMR) and dynamics of ESBL-producing UPEC isolates from UTI cases seen at a local hospital in Cusco, Peru. Ninety-nine isolates from respective patients were characterized against 18 different antibiotics. Latent class analysis (LCA) was used to evaluate the dynamics across the study time according to resistance patterns. The median age of patients was 51 years old, and nearly half were women. ESBL-producing UPEC isolates were slightly more frequent in outpatient services than emergency rooms, and there were higher resistance rates in males compared to females. Half of the ESBL producers were resistant to aminoglycosides and nitrofurantoin. Cefoxitin and fosfomycin resistance was 29.3% and 14.1%, respectively. Resistance to carbapenems was not observed. All isolates were multidrug-resistant bacteria, and 16.2% (16/99) were also classified as extensively drug-resistant bacteria. The resistance patterns varied across the study time and differed regarding sex and healthcare service. The study revealed high levels of AMR to commonly used antimicrobials and a dynamic circulation of ESBL-producing UPEC isolates with varying resistance patterns.https://www.mdpi.com/2079-6382/10/5/485urinary tract infections<i>Escherichia coli</i>β-lactamasesβ-lactam resistanceextended-spectrum β-lactamase |
| spellingShingle | Steev Loyola Fátima Concha-Velasco Jimena Pino-Dueñas Nancy Vasquez-Luna Paola Juarez Carlos Llanos Guillermo Salvatierra Jesus Tamariz Andres G. Lescano Antimicrobial Resistance Patterns and Dynamics of Extended-Spectrum β-Lactamase-Producing Uropathogenic <i>Escherichia coli</i> in Cusco, Peru Antibiotics urinary tract infections <i>Escherichia coli</i> β-lactamases β-lactam resistance extended-spectrum β-lactamase |
| title | Antimicrobial Resistance Patterns and Dynamics of Extended-Spectrum β-Lactamase-Producing Uropathogenic <i>Escherichia coli</i> in Cusco, Peru |
| title_full | Antimicrobial Resistance Patterns and Dynamics of Extended-Spectrum β-Lactamase-Producing Uropathogenic <i>Escherichia coli</i> in Cusco, Peru |
| title_fullStr | Antimicrobial Resistance Patterns and Dynamics of Extended-Spectrum β-Lactamase-Producing Uropathogenic <i>Escherichia coli</i> in Cusco, Peru |
| title_full_unstemmed | Antimicrobial Resistance Patterns and Dynamics of Extended-Spectrum β-Lactamase-Producing Uropathogenic <i>Escherichia coli</i> in Cusco, Peru |
| title_short | Antimicrobial Resistance Patterns and Dynamics of Extended-Spectrum β-Lactamase-Producing Uropathogenic <i>Escherichia coli</i> in Cusco, Peru |
| title_sort | antimicrobial resistance patterns and dynamics of extended spectrum β lactamase producing uropathogenic i escherichia coli i in cusco peru |
| topic | urinary tract infections <i>Escherichia coli</i> β-lactamases β-lactam resistance extended-spectrum β-lactamase |
| url | https://www.mdpi.com/2079-6382/10/5/485 |
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