Microbial synthesis of long-chain α-alkenes from methanol by engineering Pichia pastoris
Abstract α-Alkenes (terminal alkenes) are important fuel and platform chemicals that are mainly produced from petroleum. Microbial synthesis might provide a sustainable approach for α-alkenes. In this work, we engineered the methylotrophic yeast Pichia pastoris to produce long-chain (C15:1, C17:1 an...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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SpringerOpen
2022-05-01
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| Series: | Bioresources and Bioprocessing |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s40643-022-00551-1 |
| _version_ | 1818213922905260032 |
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| author | Peng Cai Yunxia Li Xiaoxin Zhai Lun Yao Xiaojun Ma Lingyun Jia Yongjin J. Zhou |
| author_facet | Peng Cai Yunxia Li Xiaoxin Zhai Lun Yao Xiaojun Ma Lingyun Jia Yongjin J. Zhou |
| author_sort | Peng Cai |
| collection | DOAJ |
| description | Abstract α-Alkenes (terminal alkenes) are important fuel and platform chemicals that are mainly produced from petroleum. Microbial synthesis might provide a sustainable approach for α-alkenes. In this work, we engineered the methylotrophic yeast Pichia pastoris to produce long-chain (C15:1, C17:1 and C17:2) α-alkenes via a decarboxylation of fatty acids. Combinatorial engineering, including enzyme selection, expression optimization and peroxisomal compartmentalization, enabled the production of 1.6 mg/L α-alkenes from sole methanol. This study represents the first case of α-alkene biosynthesis from methanol and also provides a reference for the construction of methanol microbial cell factories of other high-value chemicals. Graphical Abstract |
| first_indexed | 2024-12-12T06:11:59Z |
| format | Article |
| id | doaj.art-c04386a474d248c8920e4073e252b841 |
| institution | Directory Open Access Journal |
| issn | 2197-4365 |
| language | English |
| last_indexed | 2024-12-12T06:11:59Z |
| publishDate | 2022-05-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Bioresources and Bioprocessing |
| spelling | doaj.art-c04386a474d248c8920e4073e252b8412022-12-22T00:35:09ZengSpringerOpenBioresources and Bioprocessing2197-43652022-05-01911810.1186/s40643-022-00551-1Microbial synthesis of long-chain α-alkenes from methanol by engineering Pichia pastorisPeng Cai0Yunxia Li1Xiaoxin Zhai2Lun Yao3Xiaojun Ma4Lingyun Jia5Yongjin J. Zhou6School of Life Science and Biotechnology, Dalian University of TechnologyDivision of Biotechnology, Dalian Institute of Chemical Physics, Chinese Academy of SciencesDivision of Biotechnology, Dalian Institute of Chemical Physics, Chinese Academy of SciencesDalian Key Laboratory of Energy Biotechnology, Dalian Institute of Chemical Physics, Chinese Academy of SciencesDalian Key Laboratory of Energy Biotechnology, Dalian Institute of Chemical Physics, Chinese Academy of SciencesSchool of Life Science and Biotechnology, Dalian University of TechnologyDivision of Biotechnology, Dalian Institute of Chemical Physics, Chinese Academy of SciencesAbstract α-Alkenes (terminal alkenes) are important fuel and platform chemicals that are mainly produced from petroleum. Microbial synthesis might provide a sustainable approach for α-alkenes. In this work, we engineered the methylotrophic yeast Pichia pastoris to produce long-chain (C15:1, C17:1 and C17:2) α-alkenes via a decarboxylation of fatty acids. Combinatorial engineering, including enzyme selection, expression optimization and peroxisomal compartmentalization, enabled the production of 1.6 mg/L α-alkenes from sole methanol. This study represents the first case of α-alkene biosynthesis from methanol and also provides a reference for the construction of methanol microbial cell factories of other high-value chemicals. Graphical Abstracthttps://doi.org/10.1186/s40643-022-00551-1Methylotrophic yeastPichia pastorisα-AlkenesMethanol biorefineryCofactor engineeringPeroxisome |
| spellingShingle | Peng Cai Yunxia Li Xiaoxin Zhai Lun Yao Xiaojun Ma Lingyun Jia Yongjin J. Zhou Microbial synthesis of long-chain α-alkenes from methanol by engineering Pichia pastoris Bioresources and Bioprocessing Methylotrophic yeast Pichia pastoris α-Alkenes Methanol biorefinery Cofactor engineering Peroxisome |
| title | Microbial synthesis of long-chain α-alkenes from methanol by engineering Pichia pastoris |
| title_full | Microbial synthesis of long-chain α-alkenes from methanol by engineering Pichia pastoris |
| title_fullStr | Microbial synthesis of long-chain α-alkenes from methanol by engineering Pichia pastoris |
| title_full_unstemmed | Microbial synthesis of long-chain α-alkenes from methanol by engineering Pichia pastoris |
| title_short | Microbial synthesis of long-chain α-alkenes from methanol by engineering Pichia pastoris |
| title_sort | microbial synthesis of long chain α alkenes from methanol by engineering pichia pastoris |
| topic | Methylotrophic yeast Pichia pastoris α-Alkenes Methanol biorefinery Cofactor engineering Peroxisome |
| url | https://doi.org/10.1186/s40643-022-00551-1 |
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