Discovering Hair Biomarkers of Alzheimer’s Disease Using High Resolution Mass Spectrometry-Based Untargeted Metabolomics
Hair may be a potential biospecimen to discover biomarkers for Alzheimer’s disease (AD) since it reflects the integral metabolic profiles of body burden over several months. Here, we described the AD biomarker discovery in the hair using a high-resolution mass spectrometry (HRMS)-based untargeted me...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-02-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/28/5/2166 |
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| author | Yu-Hsiang Su Chih-Wei Chang Jen-Yi Hsu Shih-Wen Li Pi-Shan Sung Ru-Hsueh Wang Chih-Hsing Wu Pao-Chi Liao |
| author_facet | Yu-Hsiang Su Chih-Wei Chang Jen-Yi Hsu Shih-Wen Li Pi-Shan Sung Ru-Hsueh Wang Chih-Hsing Wu Pao-Chi Liao |
| author_sort | Yu-Hsiang Su |
| collection | DOAJ |
| description | Hair may be a potential biospecimen to discover biomarkers for Alzheimer’s disease (AD) since it reflects the integral metabolic profiles of body burden over several months. Here, we described the AD biomarker discovery in the hair using a high-resolution mass spectrometry (HRMS)-based untargeted metabolomics approach. A total of 24 patients with AD and 24 age- and sex-matched cognitively healthy controls were recruited. The hair samples were collected 0.1-cm away from the scalp and further cut into 3-cm segments. Hair metabolites were extracted by ultrasonication with methanol/phosphate-buffered saline 50/50 (<i>v</i>/<i>v</i>) for 4 h. A total of 25 discriminatory chemicals in hair between the patients with AD and controls were discovered and identified. The AUC value achieved 0.85 (95% CI: 0.72~0.97) in patients with very mild AD compared to healthy controls using a composite panel of the 9 biomarker candidates, indicating high potential for the initiation or promotion phase of AD dementia in the early stage. A metabolic panel combined with the nine metabolites may be used as biomarkers for the early detection of AD. The hair metabolome can be used to reveal metabolic perturbations for biomarker discovery. Investigating perturbations of the metabolites will offer insight into the pathogenesis of AD. |
| first_indexed | 2024-03-11T07:17:56Z |
| format | Article |
| id | doaj.art-c04c1597111c43bba59599eb9cc4f39a |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-11T07:17:56Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-c04c1597111c43bba59599eb9cc4f39a2023-11-17T08:12:55ZengMDPI AGMolecules1420-30492023-02-01285216610.3390/molecules28052166Discovering Hair Biomarkers of Alzheimer’s Disease Using High Resolution Mass Spectrometry-Based Untargeted MetabolomicsYu-Hsiang Su0Chih-Wei Chang1Jen-Yi Hsu2Shih-Wen Li3Pi-Shan Sung4Ru-Hsueh Wang5Chih-Hsing Wu6Pao-Chi Liao7Division of Neurology, Department of Internal Medicine, Ditmanson Medical Foundation Chia-Yi Christian Hospital, Chiayi City 60002, TaiwanDepartment of Environmental and Occupational Health, College of Medicine, National Cheng Kung University, Tainan 704, TaiwanDepartment of Environmental and Occupational Health, College of Medicine, National Cheng Kung University, Tainan 704, TaiwanDepartment of Environmental and Occupational Health, College of Medicine, National Cheng Kung University, Tainan 704, TaiwanDepartment of Neurology, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan 704, TaiwanDepartment of Family Medicine, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan 704, TaiwanDepartment of Family Medicine, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan 704, TaiwanDepartment of Environmental and Occupational Health, College of Medicine, National Cheng Kung University, Tainan 704, TaiwanHair may be a potential biospecimen to discover biomarkers for Alzheimer’s disease (AD) since it reflects the integral metabolic profiles of body burden over several months. Here, we described the AD biomarker discovery in the hair using a high-resolution mass spectrometry (HRMS)-based untargeted metabolomics approach. A total of 24 patients with AD and 24 age- and sex-matched cognitively healthy controls were recruited. The hair samples were collected 0.1-cm away from the scalp and further cut into 3-cm segments. Hair metabolites were extracted by ultrasonication with methanol/phosphate-buffered saline 50/50 (<i>v</i>/<i>v</i>) for 4 h. A total of 25 discriminatory chemicals in hair between the patients with AD and controls were discovered and identified. The AUC value achieved 0.85 (95% CI: 0.72~0.97) in patients with very mild AD compared to healthy controls using a composite panel of the 9 biomarker candidates, indicating high potential for the initiation or promotion phase of AD dementia in the early stage. A metabolic panel combined with the nine metabolites may be used as biomarkers for the early detection of AD. The hair metabolome can be used to reveal metabolic perturbations for biomarker discovery. Investigating perturbations of the metabolites will offer insight into the pathogenesis of AD.https://www.mdpi.com/1420-3049/28/5/2166Alzheimer’s diseaseuntargeted metabolomicshair, biomarker discoveryhigh-resolution mass spectrometry |
| spellingShingle | Yu-Hsiang Su Chih-Wei Chang Jen-Yi Hsu Shih-Wen Li Pi-Shan Sung Ru-Hsueh Wang Chih-Hsing Wu Pao-Chi Liao Discovering Hair Biomarkers of Alzheimer’s Disease Using High Resolution Mass Spectrometry-Based Untargeted Metabolomics Molecules Alzheimer’s disease untargeted metabolomics hair, biomarker discovery high-resolution mass spectrometry |
| title | Discovering Hair Biomarkers of Alzheimer’s Disease Using High Resolution Mass Spectrometry-Based Untargeted Metabolomics |
| title_full | Discovering Hair Biomarkers of Alzheimer’s Disease Using High Resolution Mass Spectrometry-Based Untargeted Metabolomics |
| title_fullStr | Discovering Hair Biomarkers of Alzheimer’s Disease Using High Resolution Mass Spectrometry-Based Untargeted Metabolomics |
| title_full_unstemmed | Discovering Hair Biomarkers of Alzheimer’s Disease Using High Resolution Mass Spectrometry-Based Untargeted Metabolomics |
| title_short | Discovering Hair Biomarkers of Alzheimer’s Disease Using High Resolution Mass Spectrometry-Based Untargeted Metabolomics |
| title_sort | discovering hair biomarkers of alzheimer s disease using high resolution mass spectrometry based untargeted metabolomics |
| topic | Alzheimer’s disease untargeted metabolomics hair, biomarker discovery high-resolution mass spectrometry |
| url | https://www.mdpi.com/1420-3049/28/5/2166 |
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