Interaction of <i>Acinetobacter baumannii</i> with Human Serum Albumin: Does the Host Determine the Outcome?
<i>Acinetobacter baumannii</i> has become a serious threat to human health due to its extreme antibiotic resistance, environmental persistence, and capacity to survive within the host. Two <i>A. baumannii</i> strains, A118 and AB5075, commonly used as model systems, and three...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-07-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/10/7/833 |
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| author | Camila Pimentel Casin Le Marisel R. Tuttobene Tomas Subils Krisztina M. Papp-Wallace Robert A. Bonomo Marcelo E. Tolmasky Maria Soledad Ramirez |
| author_facet | Camila Pimentel Casin Le Marisel R. Tuttobene Tomas Subils Krisztina M. Papp-Wallace Robert A. Bonomo Marcelo E. Tolmasky Maria Soledad Ramirez |
| author_sort | Camila Pimentel |
| collection | DOAJ |
| description | <i>Acinetobacter baumannii</i> has become a serious threat to human health due to its extreme antibiotic resistance, environmental persistence, and capacity to survive within the host. Two <i>A. baumannii</i> strains, A118 and AB5075, commonly used as model systems, and three carbapenem-resistant strains, which are becoming ever more dangerous due to the multiple drugs they can resist, were exposed to 3.5% human serum albumin (HSA) and human serum (HS) to evaluate their response with respect to antimicrobial resistance, biofilm formation, and quorum sensing, all features responsible for increasing survival and persistence in the environment and human body. Expression levels of antibiotic resistance genes were modified differently when examined in different strains. The <i>cmlA</i> gene was upregulated or downregulated in conditions of exposure to 3.5% HSA or HS depending on the strain. Expression levels of <i>pbp1</i> and <i>pbp3</i> tended to be increased by the presence of HSA and HS, but the effect was not seen in all strains. <i>A. baumannii</i> A118 growing in the presence of HS did not experience increased expression of these genes. Aminoglycoside-modifying enzymes were also expressed at higher or lower levels in the presence of HSA or HS. Still, the response was not uniform; in some cases, expression was enhanced, and in other cases, it was tapered. While <i>A. baumannii</i> AB5075 became more susceptible to rifampicin in the presence of 3.5% HSA or HS, strain A118 did not show any changes. Expression of <i>arr2,</i> a gene involved in resistance to rifampicin present in <i>A. baumannii</i> AMA16, was expressed at higher levels when HS was present in the culture medium. HSA and HS reduced biofilm formation and production of N-Acyl Homoserine Lactone, a compound intimately associated with quorum sensing. In conclusion, HSA, the main component of HS, stimulates a variety of adaptative responses in infecting <i>A. baumannii</i> strains. |
| first_indexed | 2024-03-10T09:48:17Z |
| format | Article |
| id | doaj.art-c04c27b0da374901acf9803437df5d5b |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-10T09:48:17Z |
| publishDate | 2021-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-c04c27b0da374901acf9803437df5d5b2023-11-22T03:03:56ZengMDPI AGAntibiotics2079-63822021-07-0110783310.3390/antibiotics10070833Interaction of <i>Acinetobacter baumannii</i> with Human Serum Albumin: Does the Host Determine the Outcome?Camila Pimentel0Casin Le1Marisel R. Tuttobene2Tomas Subils3Krisztina M. Papp-Wallace4Robert A. Bonomo5Marcelo E. Tolmasky6Maria Soledad Ramirez7Center for Applied Biotechnology Studies, Department of Biological Science, College of Natural Sciences and Mathematics, California State University Fullerton, Fullerton, CA 92831-3599, USACenter for Applied Biotechnology Studies, Department of Biological Science, College of Natural Sciences and Mathematics, California State University Fullerton, Fullerton, CA 92831-3599, USAÁrea Biología Molecular, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Rosario 2000, ArgentinaInstituto de Procesos Biotecnológicos y Químicos de Rosario (IPROBYQ, CONICET-UNR), Rosario S2002LRK, ArgentinaResearch Service and GRECC, Louis Stokes Cleveland Department of Veterans Affairs Medical Center, Cleveland, OH 44106, USAResearch Service and GRECC, Louis Stokes Cleveland Department of Veterans Affairs Medical Center, Cleveland, OH 44106, USACenter for Applied Biotechnology Studies, Department of Biological Science, College of Natural Sciences and Mathematics, California State University Fullerton, Fullerton, CA 92831-3599, USACenter for Applied Biotechnology Studies, Department of Biological Science, College of Natural Sciences and Mathematics, California State University Fullerton, Fullerton, CA 92831-3599, USA<i>Acinetobacter baumannii</i> has become a serious threat to human health due to its extreme antibiotic resistance, environmental persistence, and capacity to survive within the host. Two <i>A. baumannii</i> strains, A118 and AB5075, commonly used as model systems, and three carbapenem-resistant strains, which are becoming ever more dangerous due to the multiple drugs they can resist, were exposed to 3.5% human serum albumin (HSA) and human serum (HS) to evaluate their response with respect to antimicrobial resistance, biofilm formation, and quorum sensing, all features responsible for increasing survival and persistence in the environment and human body. Expression levels of antibiotic resistance genes were modified differently when examined in different strains. The <i>cmlA</i> gene was upregulated or downregulated in conditions of exposure to 3.5% HSA or HS depending on the strain. Expression levels of <i>pbp1</i> and <i>pbp3</i> tended to be increased by the presence of HSA and HS, but the effect was not seen in all strains. <i>A. baumannii</i> A118 growing in the presence of HS did not experience increased expression of these genes. Aminoglycoside-modifying enzymes were also expressed at higher or lower levels in the presence of HSA or HS. Still, the response was not uniform; in some cases, expression was enhanced, and in other cases, it was tapered. While <i>A. baumannii</i> AB5075 became more susceptible to rifampicin in the presence of 3.5% HSA or HS, strain A118 did not show any changes. Expression of <i>arr2,</i> a gene involved in resistance to rifampicin present in <i>A. baumannii</i> AMA16, was expressed at higher levels when HS was present in the culture medium. HSA and HS reduced biofilm formation and production of N-Acyl Homoserine Lactone, a compound intimately associated with quorum sensing. In conclusion, HSA, the main component of HS, stimulates a variety of adaptative responses in infecting <i>A. baumannii</i> strains.https://www.mdpi.com/2079-6382/10/7/833<i>Acinetobacter baumannii</i>antibiotic resistancequorum sensingbiofilmhuman serumalbumin |
| spellingShingle | Camila Pimentel Casin Le Marisel R. Tuttobene Tomas Subils Krisztina M. Papp-Wallace Robert A. Bonomo Marcelo E. Tolmasky Maria Soledad Ramirez Interaction of <i>Acinetobacter baumannii</i> with Human Serum Albumin: Does the Host Determine the Outcome? Antibiotics <i>Acinetobacter baumannii</i> antibiotic resistance quorum sensing biofilm human serum albumin |
| title | Interaction of <i>Acinetobacter baumannii</i> with Human Serum Albumin: Does the Host Determine the Outcome? |
| title_full | Interaction of <i>Acinetobacter baumannii</i> with Human Serum Albumin: Does the Host Determine the Outcome? |
| title_fullStr | Interaction of <i>Acinetobacter baumannii</i> with Human Serum Albumin: Does the Host Determine the Outcome? |
| title_full_unstemmed | Interaction of <i>Acinetobacter baumannii</i> with Human Serum Albumin: Does the Host Determine the Outcome? |
| title_short | Interaction of <i>Acinetobacter baumannii</i> with Human Serum Albumin: Does the Host Determine the Outcome? |
| title_sort | interaction of i acinetobacter baumannii i with human serum albumin does the host determine the outcome |
| topic | <i>Acinetobacter baumannii</i> antibiotic resistance quorum sensing biofilm human serum albumin |
| url | https://www.mdpi.com/2079-6382/10/7/833 |
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