Determination of Phenolic Compounds by Capillary Zone Electrophoresis–Mass Spectrometry
A CZE-MS method was developed for the determination of several phenolic compounds (phenolic acids, flavonoids). Since the analysis of these components necessitates the application of basic conditions for CZE separation and negative ionization mode for MS detection, the simplest choice was to use 0.5...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-07-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/27/14/4540 |
| _version_ | 1797416751000977408 |
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| author | Ruben Szabo Attila Gaspar |
| author_facet | Ruben Szabo Attila Gaspar |
| author_sort | Ruben Szabo |
| collection | DOAJ |
| description | A CZE-MS method was developed for the determination of several phenolic compounds (phenolic acids, flavonoids). Since the analysis of these components necessitates the application of basic conditions for CZE separation and negative ionization mode for MS detection, the simplest choice was to use 0.5 M NH<sub>4</sub>OH and IPA:water (1:1 <i>v</i>/<i>v</i>%) as the background electrolyte and sheath liquid, respectively. The LOD values ranged between 0.004–1.9 mg/L showing that there are relatively large differences in the ionization (and chemical) features of these compounds. The precision data were better than 0.75 RSD% for migration times and were between 5–8 RSD% for peak areas. In order to test the applicability of the developed method, a honey sample was analyzed. |
| first_indexed | 2024-03-09T06:07:35Z |
| format | Article |
| id | doaj.art-c04cb4671fb54cc6b217474ace73bf46 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T06:07:35Z |
| publishDate | 2022-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-c04cb4671fb54cc6b217474ace73bf462023-12-03T12:01:55ZengMDPI AGMolecules1420-30492022-07-012714454010.3390/molecules27144540Determination of Phenolic Compounds by Capillary Zone Electrophoresis–Mass SpectrometryRuben Szabo0Attila Gaspar1Department of Inorganic and Analytical Chemistry, University of Debrecen, Egyetem ter 1, 4032 Debrecen, HungaryDepartment of Inorganic and Analytical Chemistry, University of Debrecen, Egyetem ter 1, 4032 Debrecen, HungaryA CZE-MS method was developed for the determination of several phenolic compounds (phenolic acids, flavonoids). Since the analysis of these components necessitates the application of basic conditions for CZE separation and negative ionization mode for MS detection, the simplest choice was to use 0.5 M NH<sub>4</sub>OH and IPA:water (1:1 <i>v</i>/<i>v</i>%) as the background electrolyte and sheath liquid, respectively. The LOD values ranged between 0.004–1.9 mg/L showing that there are relatively large differences in the ionization (and chemical) features of these compounds. The precision data were better than 0.75 RSD% for migration times and were between 5–8 RSD% for peak areas. In order to test the applicability of the developed method, a honey sample was analyzed.https://www.mdpi.com/1420-3049/27/14/4540phenolic compoundscapillary zone electrophoresismass spectrometryhoney |
| spellingShingle | Ruben Szabo Attila Gaspar Determination of Phenolic Compounds by Capillary Zone Electrophoresis–Mass Spectrometry Molecules phenolic compounds capillary zone electrophoresis mass spectrometry honey |
| title | Determination of Phenolic Compounds by Capillary Zone Electrophoresis–Mass Spectrometry |
| title_full | Determination of Phenolic Compounds by Capillary Zone Electrophoresis–Mass Spectrometry |
| title_fullStr | Determination of Phenolic Compounds by Capillary Zone Electrophoresis–Mass Spectrometry |
| title_full_unstemmed | Determination of Phenolic Compounds by Capillary Zone Electrophoresis–Mass Spectrometry |
| title_short | Determination of Phenolic Compounds by Capillary Zone Electrophoresis–Mass Spectrometry |
| title_sort | determination of phenolic compounds by capillary zone electrophoresis mass spectrometry |
| topic | phenolic compounds capillary zone electrophoresis mass spectrometry honey |
| url | https://www.mdpi.com/1420-3049/27/14/4540 |
| work_keys_str_mv | AT rubenszabo determinationofphenoliccompoundsbycapillaryzoneelectrophoresismassspectrometry AT attilagaspar determinationofphenoliccompoundsbycapillaryzoneelectrophoresismassspectrometry |