Molecular detection of Leishmania infantum DNA according to clinical stages of leishmaniasis in dog
Abstract The aim of this study was to compare molecular tests used to diagnose Leishmania spp. in dogs with different stages of infection. Blood and conjunctival swab (CS) samples from dogs classified in four clinical stages were subjected to different PCR protocols (13A/13B, MC1/MC2, LITSR/L5.8S an...
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| Format: | Article |
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Colégio Brasileiro de Parasitologia Veterinaria
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| Series: | Revista Brasileira de Parasitologia Veterinária |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612019005007116&lng=en&tlng=en |
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| author | Murilo Antônio Fernandes João Augusto Franco Leonel Jéssica Anzolin Isaac Julia Cristina Benassi Diogo Tiago Silva Julio Cesar Pereira Spada Nuno Wolfgang Balbini Pereira Helena Lage Ferreira Lara Borges Keid Rodrigo Martins Soares Trícia Maria Ferreira de Sousa Oliveira |
| author_facet | Murilo Antônio Fernandes João Augusto Franco Leonel Jéssica Anzolin Isaac Julia Cristina Benassi Diogo Tiago Silva Julio Cesar Pereira Spada Nuno Wolfgang Balbini Pereira Helena Lage Ferreira Lara Borges Keid Rodrigo Martins Soares Trícia Maria Ferreira de Sousa Oliveira |
| author_sort | Murilo Antônio Fernandes |
| collection | DOAJ |
| description | Abstract The aim of this study was to compare molecular tests used to diagnose Leishmania spp. in dogs with different stages of infection. Blood and conjunctival swab (CS) samples from dogs classified in four clinical stages were subjected to different PCR protocols (13A/13B, MC1/MC2, LITSR/L5.8S and LEISH-1/LEISH-2 primers). To the study, 22.3% (48/215) of dogs were classified as without clinical signs, 67.5% (145/215) stage I (mild disease), 7.0% (15/215) stage II (moderate disease) and 3.2% (7/215) stage III (severe disease). The results showed that in blood samples, 13A/13B detected a significant higher number of positive dogs in stage I (25/145) and in total (42/215) (p≤0.05). However, when CS samples were tested, no difference was observed (p>0.05). On the other hand, in blood samples, MC1/MC2 detected significantly fewer positive dogs classified as without clinical signs (0/48), in stage I (0/145) and in total (1/215) (p≤0.05). Likewise, in CS samples, this primers showed also lower detection (1/215) (p≤0.05). So than, we can conclude that PCR on blood samples with 13A/13B primers has greater capacity to detect positive dogs, mainly at the initial of clinical disease than do other primers and MC1/MC2 are not a good choice to detect Leishmania infantum infection in dogs. |
| first_indexed | 2024-12-10T10:43:08Z |
| format | Article |
| id | doaj.art-c04d49a1598b47d2a065f5764ad7d3a1 |
| institution | Directory Open Access Journal |
| issn | 1984-2961 |
| language | English |
| last_indexed | 2024-12-10T10:43:08Z |
| publisher | Colégio Brasileiro de Parasitologia Veterinaria |
| record_format | Article |
| series | Revista Brasileira de Parasitologia Veterinária |
| spelling | doaj.art-c04d49a1598b47d2a065f5764ad7d3a12022-12-22T01:52:15ZengColégio Brasileiro de Parasitologia VeterinariaRevista Brasileira de Parasitologia Veterinária1984-2961010.1590/s1984-29612019015S1984-29612019005007116Molecular detection of Leishmania infantum DNA according to clinical stages of leishmaniasis in dogMurilo Antônio FernandesJoão Augusto Franco LeonelJéssica Anzolin IsaacJulia Cristina BenassiDiogo Tiago SilvaJulio Cesar Pereira SpadaNuno Wolfgang Balbini PereiraHelena Lage FerreiraLara Borges KeidRodrigo Martins SoaresTrícia Maria Ferreira de Sousa OliveiraAbstract The aim of this study was to compare molecular tests used to diagnose Leishmania spp. in dogs with different stages of infection. Blood and conjunctival swab (CS) samples from dogs classified in four clinical stages were subjected to different PCR protocols (13A/13B, MC1/MC2, LITSR/L5.8S and LEISH-1/LEISH-2 primers). To the study, 22.3% (48/215) of dogs were classified as without clinical signs, 67.5% (145/215) stage I (mild disease), 7.0% (15/215) stage II (moderate disease) and 3.2% (7/215) stage III (severe disease). The results showed that in blood samples, 13A/13B detected a significant higher number of positive dogs in stage I (25/145) and in total (42/215) (p≤0.05). However, when CS samples were tested, no difference was observed (p>0.05). On the other hand, in blood samples, MC1/MC2 detected significantly fewer positive dogs classified as without clinical signs (0/48), in stage I (0/145) and in total (1/215) (p≤0.05). Likewise, in CS samples, this primers showed also lower detection (1/215) (p≤0.05). So than, we can conclude that PCR on blood samples with 13A/13B primers has greater capacity to detect positive dogs, mainly at the initial of clinical disease than do other primers and MC1/MC2 are not a good choice to detect Leishmania infantum infection in dogs.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612019005007116&lng=en&tlng=enLeishmaniose visceral caninacãoLeishmania spp.PCRestágios da doença |
| spellingShingle | Murilo Antônio Fernandes João Augusto Franco Leonel Jéssica Anzolin Isaac Julia Cristina Benassi Diogo Tiago Silva Julio Cesar Pereira Spada Nuno Wolfgang Balbini Pereira Helena Lage Ferreira Lara Borges Keid Rodrigo Martins Soares Trícia Maria Ferreira de Sousa Oliveira Molecular detection of Leishmania infantum DNA according to clinical stages of leishmaniasis in dog Revista Brasileira de Parasitologia Veterinária Leishmaniose visceral canina cão Leishmania spp. PCR estágios da doença |
| title | Molecular detection of Leishmania infantum DNA according to clinical stages of leishmaniasis in dog |
| title_full | Molecular detection of Leishmania infantum DNA according to clinical stages of leishmaniasis in dog |
| title_fullStr | Molecular detection of Leishmania infantum DNA according to clinical stages of leishmaniasis in dog |
| title_full_unstemmed | Molecular detection of Leishmania infantum DNA according to clinical stages of leishmaniasis in dog |
| title_short | Molecular detection of Leishmania infantum DNA according to clinical stages of leishmaniasis in dog |
| title_sort | molecular detection of leishmania infantum dna according to clinical stages of leishmaniasis in dog |
| topic | Leishmaniose visceral canina cão Leishmania spp. PCR estágios da doença |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612019005007116&lng=en&tlng=en |
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