COULD RAPIDLY MUTATING Y-STRS BE A POTENTIAL FORENSIC TOOL IN DISCRIMINATING LEBANESE MONOZYGOTIC TWINS?
Despite the high power of discrimination that characterizes the well identified 16 to 24 autosomal short tandem repeat markers, monozygotic twins differentiation is generally limited. Arising from a single fertilized egg, monozygotic twins share the same genotype therefore the same DNA profile. Thi...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
University of Sarajevo, Institute for Genetic Engineering and Biotechnology
2018-12-01
|
| Series: | Genetics & Applications |
| Subjects: | |
| Online Access: | https://genapp.ba/editions/index.php/journal/article/view/104 |
| _version_ | 1828120987076395008 |
|---|---|
| author | Paula Romanos Jamilah Borjac |
| author_facet | Paula Romanos Jamilah Borjac |
| author_sort | Paula Romanos |
| collection | DOAJ |
| description |
Despite the high power of discrimination that characterizes the well identified 16 to 24 autosomal short tandem repeat markers, monozygotic twins differentiation is generally limited. Arising from a single fertilized egg, monozygotic twins share the same genotype therefore the same DNA profile. This situation imposes a challenge in forensics especially considering that lineage markers are in general less informative than autosomal ones. Although in some cases Y haplotype is considered a powerful investigative tool, it cannot distinguish males belonging to the same paternal lineage. The use of rapidly mutating Y-STRs with mutation rate above 1x10-2 that were recently included in forensic casework is presumed helpful. Since science is always dynamic and each population has its own characteristics, we aim in this study to distinguish between Lebanese monozygotic male twins using rapidly mutating Y-STRs. For this purpose, fourteen unrelated pairs of male monozygotic twins were recruited. Participants filled in a well-designed questionnaire and signed an informed consent. DNA was extracted using PureLink Genomic DNA Mini kit, genotyped using the Identifiler Plus kit, and separated on 3500 Genetic Analyzer to confirm the monozygosity status. DNA samples underwent a second amplification using the Y Filer Plus kit. According to our results, all the Y Filer Plus DNA profiles showed complete match for each twin pair. By consequence, the use of rapidly mutating Y-STRs in this study did not improve discrimination.
|
| first_indexed | 2024-04-11T14:15:00Z |
| format | Article |
| id | doaj.art-c04d8b9898204fe6b788741387070b9e |
| institution | Directory Open Access Journal |
| issn | 2566-2937 2566-431X |
| language | English |
| last_indexed | 2024-04-11T14:15:00Z |
| publishDate | 2018-12-01 |
| publisher | University of Sarajevo, Institute for Genetic Engineering and Biotechnology |
| record_format | Article |
| series | Genetics & Applications |
| spelling | doaj.art-c04d8b9898204fe6b788741387070b9e2022-12-22T04:19:33ZengUniversity of Sarajevo, Institute for Genetic Engineering and BiotechnologyGenetics & Applications2566-29372566-431X2018-12-0122COULD RAPIDLY MUTATING Y-STRS BE A POTENTIAL FORENSIC TOOL IN DISCRIMINATING LEBANESE MONOZYGOTIC TWINS?Paula Romanos0Jamilah Borjac1Beirut Arab University, Faculty of Sciences, Debbieh, LebanonBeirut Arab University, Faculty of Sciences, Debbieh, Lebanon Despite the high power of discrimination that characterizes the well identified 16 to 24 autosomal short tandem repeat markers, monozygotic twins differentiation is generally limited. Arising from a single fertilized egg, monozygotic twins share the same genotype therefore the same DNA profile. This situation imposes a challenge in forensics especially considering that lineage markers are in general less informative than autosomal ones. Although in some cases Y haplotype is considered a powerful investigative tool, it cannot distinguish males belonging to the same paternal lineage. The use of rapidly mutating Y-STRs with mutation rate above 1x10-2 that were recently included in forensic casework is presumed helpful. Since science is always dynamic and each population has its own characteristics, we aim in this study to distinguish between Lebanese monozygotic male twins using rapidly mutating Y-STRs. For this purpose, fourteen unrelated pairs of male monozygotic twins were recruited. Participants filled in a well-designed questionnaire and signed an informed consent. DNA was extracted using PureLink Genomic DNA Mini kit, genotyped using the Identifiler Plus kit, and separated on 3500 Genetic Analyzer to confirm the monozygosity status. DNA samples underwent a second amplification using the Y Filer Plus kit. According to our results, all the Y Filer Plus DNA profiles showed complete match for each twin pair. By consequence, the use of rapidly mutating Y-STRs in this study did not improve discrimination. https://genapp.ba/editions/index.php/journal/article/view/104Monozygotic male twinsLebanese populationdiscriminationrapidly mutating Y-STRsforensic tool |
| spellingShingle | Paula Romanos Jamilah Borjac COULD RAPIDLY MUTATING Y-STRS BE A POTENTIAL FORENSIC TOOL IN DISCRIMINATING LEBANESE MONOZYGOTIC TWINS? Genetics & Applications Monozygotic male twins Lebanese population discrimination rapidly mutating Y-STRs forensic tool |
| title | COULD RAPIDLY MUTATING Y-STRS BE A POTENTIAL FORENSIC TOOL IN DISCRIMINATING LEBANESE MONOZYGOTIC TWINS? |
| title_full | COULD RAPIDLY MUTATING Y-STRS BE A POTENTIAL FORENSIC TOOL IN DISCRIMINATING LEBANESE MONOZYGOTIC TWINS? |
| title_fullStr | COULD RAPIDLY MUTATING Y-STRS BE A POTENTIAL FORENSIC TOOL IN DISCRIMINATING LEBANESE MONOZYGOTIC TWINS? |
| title_full_unstemmed | COULD RAPIDLY MUTATING Y-STRS BE A POTENTIAL FORENSIC TOOL IN DISCRIMINATING LEBANESE MONOZYGOTIC TWINS? |
| title_short | COULD RAPIDLY MUTATING Y-STRS BE A POTENTIAL FORENSIC TOOL IN DISCRIMINATING LEBANESE MONOZYGOTIC TWINS? |
| title_sort | could rapidly mutating y strs be a potential forensic tool in discriminating lebanese monozygotic twins |
| topic | Monozygotic male twins Lebanese population discrimination rapidly mutating Y-STRs forensic tool |
| url | https://genapp.ba/editions/index.php/journal/article/view/104 |
| work_keys_str_mv | AT paularomanos couldrapidlymutatingystrsbeapotentialforensictoolindiscriminatinglebanesemonozygotictwins AT jamilahborjac couldrapidlymutatingystrsbeapotentialforensictoolindiscriminatinglebanesemonozygotictwins |