Effect of Biosynthesized Silver Nanoparticles on the Growth of the Green Microalga <i>Haematococcus pluvialis</i> and Astaxanthin Synthesis
Silver nanoparticles (AgNPs) are widely known for their antimicrobial activity in various systems from microorganisms to cell cultures. However, the data on their effects on microalgae are very limited. Unicellular green algae <i>Haematococcus pluvialis</i> is known for its ability to ac...
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MDPI AG
2023-05-01
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| Series: | Nanomaterials |
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| Online Access: | https://www.mdpi.com/2079-4991/13/10/1618 |
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| author | Petras Venckus Ieva Endriukaitytė Kotryna Čekuolytė Renata Gudiukaitė Andrius Pakalniškis Eglė Lastauskienė |
| author_facet | Petras Venckus Ieva Endriukaitytė Kotryna Čekuolytė Renata Gudiukaitė Andrius Pakalniškis Eglė Lastauskienė |
| author_sort | Petras Venckus |
| collection | DOAJ |
| description | Silver nanoparticles (AgNPs) are widely known for their antimicrobial activity in various systems from microorganisms to cell cultures. However, the data on their effects on microalgae are very limited. Unicellular green algae <i>Haematococcus pluvialis</i> is known for its ability to accumulate large amounts of astaxanthin under stress conditions. Therefore, it can be used as a suitable model system to test the influence of AgNPs on stress induction in unicellular algae, with visible phenotypic effects, such as astaxanthin synthesis and cell morphology. This study tested different AgNP concentrations (0–8 mg/L) effects on different growth stages (red and green) of <i>H. pluvialis</i> culture. Effects on cell morphology, culture productivity, and astaxanthin synthesis were evaluated. Data showed that the addition of high concentrations of AgNPs to the growing culture had a significant negative impact on culture productivity. Green-stage (HpG) cultures productivity was reduced by up to 85% by increasing AgNPs concentration to 8 mg/L while the impact on red-stage (HpR) culture was lower. Astaxanthin concentration measurements showed that AgNPs do not have any effect on astaxanthin concentration in HpG culture and caused decreased astaxanthin production rate in HpR culture. HpG culture astaxanthin concentration stayed constant at ~0.43% dry weight, while HpR culture astaxanthin concentration was significantly reduced from 1.89% to 0.60% dry weight by increasing AgNP concentration. AgNPs in the media lead to significant changes in cell morphology in both HpG and HpR cultures. Cell deformations and disrupted cytokinesis, as well as AgNPs and induced sexual reproduction, were observed. |
| first_indexed | 2024-03-11T03:26:45Z |
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| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-03-11T03:26:45Z |
| publishDate | 2023-05-01 |
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| record_format | Article |
| series | Nanomaterials |
| spelling | doaj.art-c0505d990f94488ba9adc4437a9d1cb72023-11-18T02:42:18ZengMDPI AGNanomaterials2079-49912023-05-011310161810.3390/nano13101618Effect of Biosynthesized Silver Nanoparticles on the Growth of the Green Microalga <i>Haematococcus pluvialis</i> and Astaxanthin SynthesisPetras Venckus0Ieva Endriukaitytė1Kotryna Čekuolytė2Renata Gudiukaitė3Andrius Pakalniškis4Eglė Lastauskienė5Institute of Biosciences, Life Sciences Center, Vilnius University, Sauletekio Ave. 7, LT-10257 Vilnius, LithuaniaInstitute of Biosciences, Life Sciences Center, Vilnius University, Sauletekio Ave. 7, LT-10257 Vilnius, LithuaniaInstitute of Biosciences, Life Sciences Center, Vilnius University, Sauletekio Ave. 7, LT-10257 Vilnius, LithuaniaInstitute of Biosciences, Life Sciences Center, Vilnius University, Sauletekio Ave. 7, LT-10257 Vilnius, LithuaniaInstitute of Chemistry, Vilnius University, Naugarduko 24, LT-03225 Vilnius, LithuaniaInstitute of Biosciences, Life Sciences Center, Vilnius University, Sauletekio Ave. 7, LT-10257 Vilnius, LithuaniaSilver nanoparticles (AgNPs) are widely known for their antimicrobial activity in various systems from microorganisms to cell cultures. However, the data on their effects on microalgae are very limited. Unicellular green algae <i>Haematococcus pluvialis</i> is known for its ability to accumulate large amounts of astaxanthin under stress conditions. Therefore, it can be used as a suitable model system to test the influence of AgNPs on stress induction in unicellular algae, with visible phenotypic effects, such as astaxanthin synthesis and cell morphology. This study tested different AgNP concentrations (0–8 mg/L) effects on different growth stages (red and green) of <i>H. pluvialis</i> culture. Effects on cell morphology, culture productivity, and astaxanthin synthesis were evaluated. Data showed that the addition of high concentrations of AgNPs to the growing culture had a significant negative impact on culture productivity. Green-stage (HpG) cultures productivity was reduced by up to 85% by increasing AgNPs concentration to 8 mg/L while the impact on red-stage (HpR) culture was lower. Astaxanthin concentration measurements showed that AgNPs do not have any effect on astaxanthin concentration in HpG culture and caused decreased astaxanthin production rate in HpR culture. HpG culture astaxanthin concentration stayed constant at ~0.43% dry weight, while HpR culture astaxanthin concentration was significantly reduced from 1.89% to 0.60% dry weight by increasing AgNP concentration. AgNPs in the media lead to significant changes in cell morphology in both HpG and HpR cultures. Cell deformations and disrupted cytokinesis, as well as AgNPs and induced sexual reproduction, were observed.https://www.mdpi.com/2079-4991/13/10/1618silver nanoparticles<i>Haematococcus pluvialis</i>astaxanthin |
| spellingShingle | Petras Venckus Ieva Endriukaitytė Kotryna Čekuolytė Renata Gudiukaitė Andrius Pakalniškis Eglė Lastauskienė Effect of Biosynthesized Silver Nanoparticles on the Growth of the Green Microalga <i>Haematococcus pluvialis</i> and Astaxanthin Synthesis Nanomaterials silver nanoparticles <i>Haematococcus pluvialis</i> astaxanthin |
| title | Effect of Biosynthesized Silver Nanoparticles on the Growth of the Green Microalga <i>Haematococcus pluvialis</i> and Astaxanthin Synthesis |
| title_full | Effect of Biosynthesized Silver Nanoparticles on the Growth of the Green Microalga <i>Haematococcus pluvialis</i> and Astaxanthin Synthesis |
| title_fullStr | Effect of Biosynthesized Silver Nanoparticles on the Growth of the Green Microalga <i>Haematococcus pluvialis</i> and Astaxanthin Synthesis |
| title_full_unstemmed | Effect of Biosynthesized Silver Nanoparticles on the Growth of the Green Microalga <i>Haematococcus pluvialis</i> and Astaxanthin Synthesis |
| title_short | Effect of Biosynthesized Silver Nanoparticles on the Growth of the Green Microalga <i>Haematococcus pluvialis</i> and Astaxanthin Synthesis |
| title_sort | effect of biosynthesized silver nanoparticles on the growth of the green microalga i haematococcus pluvialis i and astaxanthin synthesis |
| topic | silver nanoparticles <i>Haematococcus pluvialis</i> astaxanthin |
| url | https://www.mdpi.com/2079-4991/13/10/1618 |
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