Antimicrobial activities of chicken β -defensin (4 and 10) peptides against pathogenic bacteria and fungi
Host Defense Peptides (HDPs) are small cationic peptides found in several organisms. They play a vital role in innate immunity response and immunomodulatory stimulation. This investigation was designed to study the antimicrobial activities of β-defensin peptide-4 (sAvBD-4) and 10 (sAvBD-4) derived f...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2015-04-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fcimb.2015.00036/full |
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| author | Haitham Ahmed Yacoub Haitham Ahmed Yacoub Ahmed Mohamed Elazazy Ahmed Mohamed Elazazy osama A.H Abuzinadah Ahmed M. Al-Hejin Maged Mostafa Mahmoud Maged Mostafa Mahmoud steve M Harakeh |
| author_facet | Haitham Ahmed Yacoub Haitham Ahmed Yacoub Ahmed Mohamed Elazazy Ahmed Mohamed Elazazy osama A.H Abuzinadah Ahmed M. Al-Hejin Maged Mostafa Mahmoud Maged Mostafa Mahmoud steve M Harakeh |
| author_sort | Haitham Ahmed Yacoub |
| collection | DOAJ |
| description | Host Defense Peptides (HDPs) are small cationic peptides found in several organisms. They play a vital role in innate immunity response and immunomodulatory stimulation. This investigation was designed to study the antimicrobial activities of β-defensin peptide-4 (sAvBD-4) and 10 (sAvBD-4) derived from chickens against pathogenic organisms including bacteria and fungi. Ten bacterial strains and three fungal species were used in investigation. The results showed that the sAvBD-10 displayed a higher bactericidal potency against all the tested bacterial strains than that of sAvBD-4. The exhibited bactericidal activity was significant against almost the different bacterial strains at different peptide concentrations except for that of P. aeruginosa and Str. bovis strains where a moderate effect was noted. Both peptides were effective in the inactivation of fungal species tested yielding a killing rate of up to 95%. The results revealed that the synthetic peptides were resistant to salt at a concentration of 50mM NaCl. However, they lost antimicrobial potency when applied in the presence of high salt concentrations. Based on blood hemolysis studies, a little hemolytic effect was showed in the case of both peptides even when applied at high concentrations. The data obtained from this study indicated that synthetic avian peptides exhibit strong antibacterial and antifungal activity. In conclusion, future work and research should be tailored to a better understanding of the mechanisms of action of those peptides and their potential use in the pharmaceutical industry to help reduce the incidence and impact of infectious agent and be marketed as a naturally occurring antibiotic. |
| first_indexed | 2024-12-14T10:09:37Z |
| format | Article |
| id | doaj.art-c05435c3a75c4a6695058c41efd65ee2 |
| institution | Directory Open Access Journal |
| issn | 2235-2988 |
| language | English |
| last_indexed | 2024-12-14T10:09:37Z |
| publishDate | 2015-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj.art-c05435c3a75c4a6695058c41efd65ee22022-12-21T23:07:02ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882015-04-01510.3389/fcimb.2015.00036132458Antimicrobial activities of chicken β -defensin (4 and 10) peptides against pathogenic bacteria and fungiHaitham Ahmed Yacoub0Haitham Ahmed Yacoub1Ahmed Mohamed Elazazy2Ahmed Mohamed Elazazy3osama A.H Abuzinadah4Ahmed M. Al-Hejin5Maged Mostafa Mahmoud6Maged Mostafa Mahmoud7steve M Harakeh8king Abdulaziz universityNational Research Centerking Abdulaziz universityNational Research Centerking Abdulaziz universityking Abdulaziz universityking Abdulaziz universityNational Research Centerking Abdulaziz universityHost Defense Peptides (HDPs) are small cationic peptides found in several organisms. They play a vital role in innate immunity response and immunomodulatory stimulation. This investigation was designed to study the antimicrobial activities of β-defensin peptide-4 (sAvBD-4) and 10 (sAvBD-4) derived from chickens against pathogenic organisms including bacteria and fungi. Ten bacterial strains and three fungal species were used in investigation. The results showed that the sAvBD-10 displayed a higher bactericidal potency against all the tested bacterial strains than that of sAvBD-4. The exhibited bactericidal activity was significant against almost the different bacterial strains at different peptide concentrations except for that of P. aeruginosa and Str. bovis strains where a moderate effect was noted. Both peptides were effective in the inactivation of fungal species tested yielding a killing rate of up to 95%. The results revealed that the synthetic peptides were resistant to salt at a concentration of 50mM NaCl. However, they lost antimicrobial potency when applied in the presence of high salt concentrations. Based on blood hemolysis studies, a little hemolytic effect was showed in the case of both peptides even when applied at high concentrations. The data obtained from this study indicated that synthetic avian peptides exhibit strong antibacterial and antifungal activity. In conclusion, future work and research should be tailored to a better understanding of the mechanisms of action of those peptides and their potential use in the pharmaceutical industry to help reduce the incidence and impact of infectious agent and be marketed as a naturally occurring antibiotic.http://journal.frontiersin.org/Journal/10.3389/fcimb.2015.00036/fullBacteriaFungiAntimicrobial activitySynthetic AvBD-4 and 10natural antibiotic. |
| spellingShingle | Haitham Ahmed Yacoub Haitham Ahmed Yacoub Ahmed Mohamed Elazazy Ahmed Mohamed Elazazy osama A.H Abuzinadah Ahmed M. Al-Hejin Maged Mostafa Mahmoud Maged Mostafa Mahmoud steve M Harakeh Antimicrobial activities of chicken β -defensin (4 and 10) peptides against pathogenic bacteria and fungi Frontiers in Cellular and Infection Microbiology Bacteria Fungi Antimicrobial activity Synthetic AvBD-4 and 10 natural antibiotic. |
| title | Antimicrobial activities of chicken β -defensin (4 and 10) peptides against pathogenic bacteria and fungi |
| title_full | Antimicrobial activities of chicken β -defensin (4 and 10) peptides against pathogenic bacteria and fungi |
| title_fullStr | Antimicrobial activities of chicken β -defensin (4 and 10) peptides against pathogenic bacteria and fungi |
| title_full_unstemmed | Antimicrobial activities of chicken β -defensin (4 and 10) peptides against pathogenic bacteria and fungi |
| title_short | Antimicrobial activities of chicken β -defensin (4 and 10) peptides against pathogenic bacteria and fungi |
| title_sort | antimicrobial activities of chicken β defensin 4 and 10 peptides against pathogenic bacteria and fungi |
| topic | Bacteria Fungi Antimicrobial activity Synthetic AvBD-4 and 10 natural antibiotic. |
| url | http://journal.frontiersin.org/Journal/10.3389/fcimb.2015.00036/full |
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