The Effect of Cannabidiol on UV-Induced Changes in Intracellular Signaling of 3D-Cultured Skin Keratinocytes
Human epidermal keratinocytes are constantly exposed to UV radiation. As a result, there is a significant need for safe and effective compounds to protect skin cells against this environmental damage. This study aimed to analyze the effect of phytocannabinoid-cannabinoid (CBD)-on the proteome of UVA...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-02-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/3/1501 |
| _version_ | 1797416319920898048 |
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| author | Agnieszka Gęgotek Sinemyiz Atalay Adelina Rogowska-Wrzesińska Elżbieta Skrzydlewska |
| author_facet | Agnieszka Gęgotek Sinemyiz Atalay Adelina Rogowska-Wrzesińska Elżbieta Skrzydlewska |
| author_sort | Agnieszka Gęgotek |
| collection | DOAJ |
| description | Human epidermal keratinocytes are constantly exposed to UV radiation. As a result, there is a significant need for safe and effective compounds to protect skin cells against this environmental damage. This study aimed to analyze the effect of phytocannabinoid-cannabinoid (CBD)-on the proteome of UVA/B irradiated keratinocytes. The keratinocytes were cultured in a three-dimensional (3D) system, designed to mimic epidermal conditions closely. The obtained results indicate that CBD protected against the harmful effects of UVA/B radiation. CBD decreased the expression of proinflammatory proteins, including TNFα/NFκB and IκBKB complex and decreased the expression of proteins involved in de novo protein biosynthesis, which are increased in UVA/B-irradiated cells. Additionally, CBD enhanced the UV-induced expression of 20S proteasome subunits. CBD also protected protein structures from 4-hydroxynonenal (HNE)-binding induced by UV radiation, which primarily affects antioxidant enzymes. CBD-through its antioxidant/anti-inflammatory activity and regulation of protein biosynthesis and degradation-protects skin cells against UVA/B-induced changes. In the future, its long-term use in epidermal cells should be investigated. |
| first_indexed | 2024-03-09T06:01:38Z |
| format | Article |
| id | doaj.art-c054b1d818964b71ad2f69ac8656c870 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T06:01:38Z |
| publishDate | 2021-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-c054b1d818964b71ad2f69ac8656c8702023-12-03T12:08:59ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-02-01223150110.3390/ijms22031501The Effect of Cannabidiol on UV-Induced Changes in Intracellular Signaling of 3D-Cultured Skin KeratinocytesAgnieszka Gęgotek0Sinemyiz Atalay1Adelina Rogowska-Wrzesińska2Elżbieta Skrzydlewska3Department of Analytical Chemistry, Medical University of Bialystok, Mickiewicza 2D, 15-222 Bialystok, PolandDepartment of Analytical Chemistry, Medical University of Bialystok, Mickiewicza 2D, 15-222 Bialystok, PolandDepartment of Biochemistry and Molecular Biology, University of Southern Danmark, Campusvej 55, DK-5230 Odense M, DenmarkDepartment of Analytical Chemistry, Medical University of Bialystok, Mickiewicza 2D, 15-222 Bialystok, PolandHuman epidermal keratinocytes are constantly exposed to UV radiation. As a result, there is a significant need for safe and effective compounds to protect skin cells against this environmental damage. This study aimed to analyze the effect of phytocannabinoid-cannabinoid (CBD)-on the proteome of UVA/B irradiated keratinocytes. The keratinocytes were cultured in a three-dimensional (3D) system, designed to mimic epidermal conditions closely. The obtained results indicate that CBD protected against the harmful effects of UVA/B radiation. CBD decreased the expression of proinflammatory proteins, including TNFα/NFκB and IκBKB complex and decreased the expression of proteins involved in de novo protein biosynthesis, which are increased in UVA/B-irradiated cells. Additionally, CBD enhanced the UV-induced expression of 20S proteasome subunits. CBD also protected protein structures from 4-hydroxynonenal (HNE)-binding induced by UV radiation, which primarily affects antioxidant enzymes. CBD-through its antioxidant/anti-inflammatory activity and regulation of protein biosynthesis and degradation-protects skin cells against UVA/B-induced changes. In the future, its long-term use in epidermal cells should be investigated.https://www.mdpi.com/1422-0067/22/3/1501cannabidiolUV irradiated skinthree-dimensional in vitro culturekeratinocytesproteomicslipid peroxidation products adducts formation |
| spellingShingle | Agnieszka Gęgotek Sinemyiz Atalay Adelina Rogowska-Wrzesińska Elżbieta Skrzydlewska The Effect of Cannabidiol on UV-Induced Changes in Intracellular Signaling of 3D-Cultured Skin Keratinocytes International Journal of Molecular Sciences cannabidiol UV irradiated skin three-dimensional in vitro culture keratinocytes proteomics lipid peroxidation products adducts formation |
| title | The Effect of Cannabidiol on UV-Induced Changes in Intracellular Signaling of 3D-Cultured Skin Keratinocytes |
| title_full | The Effect of Cannabidiol on UV-Induced Changes in Intracellular Signaling of 3D-Cultured Skin Keratinocytes |
| title_fullStr | The Effect of Cannabidiol on UV-Induced Changes in Intracellular Signaling of 3D-Cultured Skin Keratinocytes |
| title_full_unstemmed | The Effect of Cannabidiol on UV-Induced Changes in Intracellular Signaling of 3D-Cultured Skin Keratinocytes |
| title_short | The Effect of Cannabidiol on UV-Induced Changes in Intracellular Signaling of 3D-Cultured Skin Keratinocytes |
| title_sort | effect of cannabidiol on uv induced changes in intracellular signaling of 3d cultured skin keratinocytes |
| topic | cannabidiol UV irradiated skin three-dimensional in vitro culture keratinocytes proteomics lipid peroxidation products adducts formation |
| url | https://www.mdpi.com/1422-0067/22/3/1501 |
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