Establishment and Characterization of Feline Mammary Tumor Patient-Derived Xenograft Model
Feline mammary tumor is a relatively commonly diagnosed neoplasm in the cat. Development of new veterinary cancer therapies is limited by the shortage of in vivo models that reproduce tumor microenvironment and metastatic progression. Four feline mammary tumor orthotopic patient-derived xenograft mo...
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| Format: | Article |
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MDPI AG
2021-08-01
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| Online Access: | https://www.mdpi.com/2076-2615/11/8/2380 |
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| author | Hsiao-Li Chuang Yi-Chih Chang Yi-Ting Huang Jiunn-Wang Liao Pei-Ling Kao Yi-Fei Chen Bin-Yin Lin Yi-Lo Lin Ter-Hsin Chen Yu-Chih Wang |
| author_facet | Hsiao-Li Chuang Yi-Chih Chang Yi-Ting Huang Jiunn-Wang Liao Pei-Ling Kao Yi-Fei Chen Bin-Yin Lin Yi-Lo Lin Ter-Hsin Chen Yu-Chih Wang |
| author_sort | Hsiao-Li Chuang |
| collection | DOAJ |
| description | Feline mammary tumor is a relatively commonly diagnosed neoplasm in the cat. Development of new veterinary cancer therapies is limited by the shortage of in vivo models that reproduce tumor microenvironment and metastatic progression. Four feline mammary tumor orthotopic patient-derived xenograft model (PDX) successfully established in NOD-SCID gamma (NSG) mice. The overall success rate of PDX establishment was 36% (4/11). Histological, immunohistochemical, and short tandem repeat analysis showed a remarkable similarity between patient’s tumor and xenograft. The tumor grafts conserve original tumor essential features, including distant metastasis. Primary FMT-1807 cell line isolated from FMT-1807PDX tumor tissue. Tumorigenicity of FMT-1807 cells expanded from PDX was assessed by orthotopic injection into NSG mice. Mice yielded tumors which preserve the lung and liver metastasis ability. This work provides a platform for FMT translational investigation. |
| first_indexed | 2024-03-10T09:04:38Z |
| format | Article |
| id | doaj.art-c0576041424a43e092cb2e1875b01139 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-10T09:04:38Z |
| publishDate | 2021-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-c0576041424a43e092cb2e1875b011392023-11-22T06:31:16ZengMDPI AGAnimals2076-26152021-08-01118238010.3390/ani11082380Establishment and Characterization of Feline Mammary Tumor Patient-Derived Xenograft ModelHsiao-Li Chuang0Yi-Chih Chang1Yi-Ting Huang2Jiunn-Wang Liao3Pei-Ling Kao4Yi-Fei Chen5Bin-Yin Lin6Yi-Lo Lin7Ter-Hsin Chen8Yu-Chih Wang9National Laboratory Animal Center, National Applied Research Laboratories, Taipei 106214, TaiwanDepartment of Biotechnology, College of Medical and Health Science, Asia University, Taichung 41354, TaiwanGraduate Institute of Veterinary Pathobiology, National Chung Hsing University, Taichung 402, TaiwanGraduate Institute of Veterinary Pathobiology, National Chung Hsing University, Taichung 402, TaiwanGraduate Institute of Veterinary Pathobiology, National Chung Hsing University, Taichung 402, TaiwanGraduate Institute of Veterinary Pathobiology, National Chung Hsing University, Taichung 402, TaiwanGraduate Institute of Veterinary Pathobiology, National Chung Hsing University, Taichung 402, TaiwanGraduate Institute of Veterinary Pathobiology, National Chung Hsing University, Taichung 402, TaiwanGraduate Institute of Veterinary Pathobiology, National Chung Hsing University, Taichung 402, TaiwanGraduate Institute of Veterinary Pathobiology, National Chung Hsing University, Taichung 402, TaiwanFeline mammary tumor is a relatively commonly diagnosed neoplasm in the cat. Development of new veterinary cancer therapies is limited by the shortage of in vivo models that reproduce tumor microenvironment and metastatic progression. Four feline mammary tumor orthotopic patient-derived xenograft model (PDX) successfully established in NOD-SCID gamma (NSG) mice. The overall success rate of PDX establishment was 36% (4/11). Histological, immunohistochemical, and short tandem repeat analysis showed a remarkable similarity between patient’s tumor and xenograft. The tumor grafts conserve original tumor essential features, including distant metastasis. Primary FMT-1807 cell line isolated from FMT-1807PDX tumor tissue. Tumorigenicity of FMT-1807 cells expanded from PDX was assessed by orthotopic injection into NSG mice. Mice yielded tumors which preserve the lung and liver metastasis ability. This work provides a platform for FMT translational investigation.https://www.mdpi.com/2076-2615/11/8/2380feline mammary tumorpatient-derived xenograftcell line |
| spellingShingle | Hsiao-Li Chuang Yi-Chih Chang Yi-Ting Huang Jiunn-Wang Liao Pei-Ling Kao Yi-Fei Chen Bin-Yin Lin Yi-Lo Lin Ter-Hsin Chen Yu-Chih Wang Establishment and Characterization of Feline Mammary Tumor Patient-Derived Xenograft Model Animals feline mammary tumor patient-derived xenograft cell line |
| title | Establishment and Characterization of Feline Mammary Tumor Patient-Derived Xenograft Model |
| title_full | Establishment and Characterization of Feline Mammary Tumor Patient-Derived Xenograft Model |
| title_fullStr | Establishment and Characterization of Feline Mammary Tumor Patient-Derived Xenograft Model |
| title_full_unstemmed | Establishment and Characterization of Feline Mammary Tumor Patient-Derived Xenograft Model |
| title_short | Establishment and Characterization of Feline Mammary Tumor Patient-Derived Xenograft Model |
| title_sort | establishment and characterization of feline mammary tumor patient derived xenograft model |
| topic | feline mammary tumor patient-derived xenograft cell line |
| url | https://www.mdpi.com/2076-2615/11/8/2380 |
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