The structure of gut microbiome in idiopathic short stature profiled by 16S rRNA second generation sequencing sequencing
Objective: To investigate structural characteristics of gut microbiome in idiopathic short stature (ISS) children and difference in gut microbiome between ISS and healthy children(HC) children, so as to explore the possible mechanism of action of gut microbiome on ISS. Methods: A total of 16 childre...
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| Format: | Article |
| Language: | zho |
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Editorial Office of Journal of Diagnostics Concepts & Practice
2021-04-01
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| Series: | Zhenduanxue lilun yu shijian |
| Subjects: | |
| Online Access: | http://www.qk.sjtu.edu.cn/jdcp/fileup/1671-2870/PDF/1671-2870-2021-20-2-149.pdf |
| _version_ | 1797975339604901888 |
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| author | LI Lin, AN Jingjing, WANG Junqi, WANG Xinqiong, DONG Zhiya |
| author_facet | LI Lin, AN Jingjing, WANG Junqi, WANG Xinqiong, DONG Zhiya |
| author_sort | LI Lin, AN Jingjing, WANG Junqi, WANG Xinqiong, DONG Zhiya |
| collection | DOAJ |
| description | Objective: To investigate structural characteristics of gut microbiome in idiopathic short stature (ISS) children and difference in gut microbiome between ISS and healthy children(HC) children, so as to explore the possible mechanism of action of gut microbiome on ISS. Methods: A total of 16 children aging from 4 to 8, including 16 ISS children (ISS group)and 16 HC (control group) were enrolled in the study. Flora of the collected feces were collected, and V3-V4 of bacterial 16S rRNA was sequenced with high-throughput sequencing. Diversity and abundance of gut microbiome between HC and ISS group were compared. Results: The structure of gut microbiome in ISS children were significantly different from HC children. The Chao1 (diversity index) of ISS group were lower than that of HC group (1109.85±435.23 vs. 1485.68±334.51) (P=0.018) as well as Observed_species index(946.98±374.93 vs. 1 217.69±289.66)(P=0.038), indicating decreased relative abundance of gut microbiome in ISS group. It’s also showed that the relative abundances of Faecalibacterium and Eubacterium were significantly lower in ISS group than HC group (P<0.05). In ISS group, both abundances of Faecalibacterium and Eubacterium were positively correlated with height-SDS and insulin-like growth factor 1(IGF1)-SDS（r>0.6, P<0.05）. Conclusions: For ISS, the abundance of gut microbiome decreases and the flora also experiences significant structural changes. Faecalibacterium and Eubacterium may decrease IGF-1 by short-chain fatty acid (SCFA), constituting a possible pathogenic mechanism for ISS. |
| first_indexed | 2024-04-11T04:33:57Z |
| format | Article |
| id | doaj.art-c0583ae3bee34ba19593dae88ba7838d |
| institution | Directory Open Access Journal |
| issn | 1671-2870 |
| language | zho |
| last_indexed | 2024-04-11T04:33:57Z |
| publishDate | 2021-04-01 |
| publisher | Editorial Office of Journal of Diagnostics Concepts & Practice |
| record_format | Article |
| series | Zhenduanxue lilun yu shijian |
| spelling | doaj.art-c0583ae3bee34ba19593dae88ba7838d2022-12-29T00:57:44ZzhoEditorial Office of Journal of Diagnostics Concepts & PracticeZhenduanxue lilun yu shijian1671-28702021-04-01200214915410.16150/j.1671-2870.2021.02.006The structure of gut microbiome in idiopathic short stature profiled by 16S rRNA second generation sequencing sequencingLI Lin, AN Jingjing, WANG Junqi, WANG Xinqiong, DONG Zhiya0Department of Pediatrics, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, ChinaObjective: To investigate structural characteristics of gut microbiome in idiopathic short stature (ISS) children and difference in gut microbiome between ISS and healthy children(HC) children, so as to explore the possible mechanism of action of gut microbiome on ISS. Methods: A total of 16 children aging from 4 to 8, including 16 ISS children (ISS group)and 16 HC (control group) were enrolled in the study. Flora of the collected feces were collected, and V3-V4 of bacterial 16S rRNA was sequenced with high-throughput sequencing. Diversity and abundance of gut microbiome between HC and ISS group were compared. Results: The structure of gut microbiome in ISS children were significantly different from HC children. The Chao1 (diversity index) of ISS group were lower than that of HC group (1109.85±435.23 vs. 1485.68±334.51) (P=0.018) as well as Observed_species index(946.98±374.93 vs. 1 217.69±289.66)(P=0.038), indicating decreased relative abundance of gut microbiome in ISS group. It’s also showed that the relative abundances of Faecalibacterium and Eubacterium were significantly lower in ISS group than HC group (P<0.05). In ISS group, both abundances of Faecalibacterium and Eubacterium were positively correlated with height-SDS and insulin-like growth factor 1(IGF1)-SDS（r>0.6, P<0.05）. Conclusions: For ISS, the abundance of gut microbiome decreases and the flora also experiences significant structural changes. Faecalibacterium and Eubacterium may decrease IGF-1 by short-chain fatty acid (SCFA), constituting a possible pathogenic mechanism for ISS.http://www.qk.sjtu.edu.cn/jdcp/fileup/1671-2870/PDF/1671-2870-2021-20-2-149.pdf|idiopathic short stature|gut microbiome|16s rrna |
| spellingShingle | LI Lin, AN Jingjing, WANG Junqi, WANG Xinqiong, DONG Zhiya The structure of gut microbiome in idiopathic short stature profiled by 16S rRNA second generation sequencing sequencing Zhenduanxue lilun yu shijian |idiopathic short stature|gut microbiome|16s rrna |
| title | The structure of gut microbiome in idiopathic short stature profiled by 16S rRNA second generation sequencing sequencing |
| title_full | The structure of gut microbiome in idiopathic short stature profiled by 16S rRNA second generation sequencing sequencing |
| title_fullStr | The structure of gut microbiome in idiopathic short stature profiled by 16S rRNA second generation sequencing sequencing |
| title_full_unstemmed | The structure of gut microbiome in idiopathic short stature profiled by 16S rRNA second generation sequencing sequencing |
| title_short | The structure of gut microbiome in idiopathic short stature profiled by 16S rRNA second generation sequencing sequencing |
| title_sort | structure of gut microbiome in idiopathic short stature profiled by 16s rrna second generation sequencing sequencing |
| topic | |idiopathic short stature|gut microbiome|16s rrna |
| url | http://www.qk.sjtu.edu.cn/jdcp/fileup/1671-2870/PDF/1671-2870-2021-20-2-149.pdf |
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