<it>Dianthus superbus fructus </it>suppresses airway inflammation by downregulating of inducible nitric oxide synthase in an ovalbumin-induced murine model of asthma
<p>Abstract</p> <p>Background</p> <p><it>Dianthus superbus</it> has long been used as a herbal medicine in Asia and as an anti-inflammatory agent. In this study, we evaluated the anti-inflammatory effects of <it>Dianthus superbus fructus</it> eth...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2012-10-01
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| Series: | Journal of Inflammation |
| Subjects: | |
| Online Access: | http://www.journal-inflammation.com/content/9/1/41 |
| _version_ | 1811260065519239168 |
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| author | Shin In-Sik Lee Mee-Young Ha Hyekyung Jeon Woo-Young Seo Chang-Seob Shin Hyeun-Kyoo |
| author_facet | Shin In-Sik Lee Mee-Young Ha Hyekyung Jeon Woo-Young Seo Chang-Seob Shin Hyeun-Kyoo |
| author_sort | Shin In-Sik |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p><it>Dianthus superbus</it> has long been used as a herbal medicine in Asia and as an anti-inflammatory agent. In this study, we evaluated the anti-inflammatory effects of <it>Dianthus superbus fructus</it> ethanolic extract (DSE) on Th2-type cytokines, eosinophil infiltration, and other factors in an ovalbumin (OVA)-induced murine asthma model. To study the possible mechanism of the anti-inflammatory effect of DSE, we also evaluated the expression of inducible nitric oxide synthase (iNOS) in the respiratory tract.</p> <p>Methods</p> <p>Mice were sensitized on days 0 and 14 by intraperitoneal injection of OVA. On days 21, 22 and 23 after initial sensitization, mice received an airway challenge with OVA for 1 h using an ultrasonic nebulizer. DSE was applied 1 h prior to OVA challenge. Mice were administered DSE orally at doses of 100 mg/kg or 200 mg/kg once daily from day 18 to 23. Bronchoalveolar lavage fluid (BALF) was collected 48 h after the final OVA challenge. Levels of interleukin (IL)-4, IL-13 and eotaxin in BALF were measured using enzyme-linked immunosorbent assays (ELISAs). Lung tissue sections were stained with hematoxylin and eosin for assessment of cell infiltration and mucus production with periodic acid shift staining, in conjunction with ELISA and western blot analyses for iNOS expression.</p> <p>Results</p> <p>DSE significantly reduced the levels of IL-4, IL-13, eotaxin, and immunoglobulin (Ig) E, number of inflammatory cells in BALF, and inflammatory cell infiltration and mucus production in the respiratory tract. DSE also attenuated the overexpression of iNOS protein induced by OVA challenge.</p> <p>Conclusion</p> <p>Our results suggest that DSE effectively protects against allergic airway inflammation by downregulating of iNOS expression and that DSE has potential as a therapeutic agent for allergic asthma.</p> |
| first_indexed | 2024-04-12T18:41:51Z |
| format | Article |
| id | doaj.art-c05aa51bbcb84d6cbf0c3634851cddb3 |
| institution | Directory Open Access Journal |
| issn | 1476-9255 |
| language | English |
| last_indexed | 2024-04-12T18:41:51Z |
| publishDate | 2012-10-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Inflammation |
| spelling | doaj.art-c05aa51bbcb84d6cbf0c3634851cddb32022-12-22T03:20:46ZengBMCJournal of Inflammation1476-92552012-10-01914110.1186/1476-9255-9-41<it>Dianthus superbus fructus </it>suppresses airway inflammation by downregulating of inducible nitric oxide synthase in an ovalbumin-induced murine model of asthmaShin In-SikLee Mee-YoungHa HyekyungJeon Woo-YoungSeo Chang-SeobShin Hyeun-Kyoo<p>Abstract</p> <p>Background</p> <p><it>Dianthus superbus</it> has long been used as a herbal medicine in Asia and as an anti-inflammatory agent. In this study, we evaluated the anti-inflammatory effects of <it>Dianthus superbus fructus</it> ethanolic extract (DSE) on Th2-type cytokines, eosinophil infiltration, and other factors in an ovalbumin (OVA)-induced murine asthma model. To study the possible mechanism of the anti-inflammatory effect of DSE, we also evaluated the expression of inducible nitric oxide synthase (iNOS) in the respiratory tract.</p> <p>Methods</p> <p>Mice were sensitized on days 0 and 14 by intraperitoneal injection of OVA. On days 21, 22 and 23 after initial sensitization, mice received an airway challenge with OVA for 1 h using an ultrasonic nebulizer. DSE was applied 1 h prior to OVA challenge. Mice were administered DSE orally at doses of 100 mg/kg or 200 mg/kg once daily from day 18 to 23. Bronchoalveolar lavage fluid (BALF) was collected 48 h after the final OVA challenge. Levels of interleukin (IL)-4, IL-13 and eotaxin in BALF were measured using enzyme-linked immunosorbent assays (ELISAs). Lung tissue sections were stained with hematoxylin and eosin for assessment of cell infiltration and mucus production with periodic acid shift staining, in conjunction with ELISA and western blot analyses for iNOS expression.</p> <p>Results</p> <p>DSE significantly reduced the levels of IL-4, IL-13, eotaxin, and immunoglobulin (Ig) E, number of inflammatory cells in BALF, and inflammatory cell infiltration and mucus production in the respiratory tract. DSE also attenuated the overexpression of iNOS protein induced by OVA challenge.</p> <p>Conclusion</p> <p>Our results suggest that DSE effectively protects against allergic airway inflammation by downregulating of iNOS expression and that DSE has potential as a therapeutic agent for allergic asthma.</p>http://www.journal-inflammation.com/content/9/1/41<it>Dianthus superbus fructus</it>AsthmaInflammationCytokineInducible nitric oxide synthase |
| spellingShingle | Shin In-Sik Lee Mee-Young Ha Hyekyung Jeon Woo-Young Seo Chang-Seob Shin Hyeun-Kyoo <it>Dianthus superbus fructus </it>suppresses airway inflammation by downregulating of inducible nitric oxide synthase in an ovalbumin-induced murine model of asthma Journal of Inflammation <it>Dianthus superbus fructus</it> Asthma Inflammation Cytokine Inducible nitric oxide synthase |
| title | <it>Dianthus superbus fructus </it>suppresses airway inflammation by downregulating of inducible nitric oxide synthase in an ovalbumin-induced murine model of asthma |
| title_full | <it>Dianthus superbus fructus </it>suppresses airway inflammation by downregulating of inducible nitric oxide synthase in an ovalbumin-induced murine model of asthma |
| title_fullStr | <it>Dianthus superbus fructus </it>suppresses airway inflammation by downregulating of inducible nitric oxide synthase in an ovalbumin-induced murine model of asthma |
| title_full_unstemmed | <it>Dianthus superbus fructus </it>suppresses airway inflammation by downregulating of inducible nitric oxide synthase in an ovalbumin-induced murine model of asthma |
| title_short | <it>Dianthus superbus fructus </it>suppresses airway inflammation by downregulating of inducible nitric oxide synthase in an ovalbumin-induced murine model of asthma |
| title_sort | it dianthus superbus fructus it suppresses airway inflammation by downregulating of inducible nitric oxide synthase in an ovalbumin induced murine model of asthma |
| topic | <it>Dianthus superbus fructus</it> Asthma Inflammation Cytokine Inducible nitric oxide synthase |
| url | http://www.journal-inflammation.com/content/9/1/41 |
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