Development and characterization of SSR markers for Sanguinaria canadensis based on genome skimming
Premise Polymorphic nuclear simple sequence repeat (nSSR) markers were developed for Sanguinaria canadensis (Papaveraceae), a spring ephemeral native to eastern North America. Methods and Results Based on the genome skimming data of S. canadensis, a total of 240 nSSR primer pairs were designed for 8...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2019-09-01
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| Series: | Applications in Plant Sciences |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/aps3.11289 |
| _version_ | 1818026408894529536 |
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| author | Renyu Liao Yuxin Luo Gulbar Yisilam Ruisen Lu Yuguo Wang Pan Li |
| author_facet | Renyu Liao Yuxin Luo Gulbar Yisilam Ruisen Lu Yuguo Wang Pan Li |
| author_sort | Renyu Liao |
| collection | DOAJ |
| description | Premise Polymorphic nuclear simple sequence repeat (nSSR) markers were developed for Sanguinaria canadensis (Papaveraceae), a spring ephemeral native to eastern North America. Methods and Results Based on the genome skimming data of S. canadensis, a total of 240 nSSR primer pairs were designed for 80 loci from the assembled nuclear contigs. Of these primer pairs, 19 were selected for initial validation in four populations (80 individuals). All 19 loci produced heterologous amplification. The numbers of alleles per locus ranged from one to 21; the levels of observed and expected heterozygosity per locus ranged from 0.000 to 1.000 and from 0.000 to 0.847, respectively. Transferability of the loci was tested in the related species Eomecon chionantha. Conclusions The developed nSSR markers revealed polymorphism in the four studied populations and may contribute to investigations of the genetic diversity of S. canadensis and E. chionantha. |
| first_indexed | 2024-12-10T04:31:32Z |
| format | Article |
| id | doaj.art-c05bc3b0126848aa9b968942f1559961 |
| institution | Directory Open Access Journal |
| issn | 2168-0450 |
| language | English |
| last_indexed | 2024-12-10T04:31:32Z |
| publishDate | 2019-09-01 |
| publisher | Wiley |
| record_format | Article |
| series | Applications in Plant Sciences |
| spelling | doaj.art-c05bc3b0126848aa9b968942f15599612022-12-22T02:02:07ZengWileyApplications in Plant Sciences2168-04502019-09-0179n/an/a10.1002/aps3.11289Development and characterization of SSR markers for Sanguinaria canadensis based on genome skimmingRenyu Liao0Yuxin Luo1Gulbar Yisilam2Ruisen Lu3Yuguo Wang4Pan Li5Laboratory of Systematic and Evolutionary Botany and Biodiversity College of Life Sciences Zhejiang University Hangzhou Zhejiang 310058 People's Republic of ChinaLaboratory of Systematic and Evolutionary Botany and Biodiversity College of Life Sciences Zhejiang University Hangzhou Zhejiang 310058 People's Republic of ChinaLaboratory of Systematic and Evolutionary Botany and Biodiversity College of Life Sciences Zhejiang University Hangzhou Zhejiang 310058 People's Republic of ChinaLaboratory of Systematic and Evolutionary Botany and Biodiversity College of Life Sciences Zhejiang University Hangzhou Zhejiang 310058 People's Republic of ChinaMinistry of Education Key Laboratory for Biodiversity Science and Ecological Engineering Institute of Biodiversity Science Fudan University Shanghai 200433 People's Republic of ChinaLaboratory of Systematic and Evolutionary Botany and Biodiversity College of Life Sciences Zhejiang University Hangzhou Zhejiang 310058 People's Republic of ChinaPremise Polymorphic nuclear simple sequence repeat (nSSR) markers were developed for Sanguinaria canadensis (Papaveraceae), a spring ephemeral native to eastern North America. Methods and Results Based on the genome skimming data of S. canadensis, a total of 240 nSSR primer pairs were designed for 80 loci from the assembled nuclear contigs. Of these primer pairs, 19 were selected for initial validation in four populations (80 individuals). All 19 loci produced heterologous amplification. The numbers of alleles per locus ranged from one to 21; the levels of observed and expected heterozygosity per locus ranged from 0.000 to 1.000 and from 0.000 to 0.847, respectively. Transferability of the loci was tested in the related species Eomecon chionantha. Conclusions The developed nSSR markers revealed polymorphism in the four studied populations and may contribute to investigations of the genetic diversity of S. canadensis and E. chionantha.https://doi.org/10.1002/aps3.11289eastern North AmericaEomeconmicrosatellitesPapaveraceaeSanguinaria canadensis |
| spellingShingle | Renyu Liao Yuxin Luo Gulbar Yisilam Ruisen Lu Yuguo Wang Pan Li Development and characterization of SSR markers for Sanguinaria canadensis based on genome skimming Applications in Plant Sciences eastern North America Eomecon microsatellites Papaveraceae Sanguinaria canadensis |
| title | Development and characterization of SSR markers for Sanguinaria canadensis based on genome skimming |
| title_full | Development and characterization of SSR markers for Sanguinaria canadensis based on genome skimming |
| title_fullStr | Development and characterization of SSR markers for Sanguinaria canadensis based on genome skimming |
| title_full_unstemmed | Development and characterization of SSR markers for Sanguinaria canadensis based on genome skimming |
| title_short | Development and characterization of SSR markers for Sanguinaria canadensis based on genome skimming |
| title_sort | development and characterization of ssr markers for sanguinaria canadensis based on genome skimming |
| topic | eastern North America Eomecon microsatellites Papaveraceae Sanguinaria canadensis |
| url | https://doi.org/10.1002/aps3.11289 |
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