Tunicamycin Sensitivity-Suppression by High Gene Dosage Reveals New Functions of the Yeast Hog1 MAP Kinase
In the yeast <i>Saccharomyces cerevisiae</i>, components of the High Osmolarity Glycerol (HOG) pathway are important for the response to diverse stresses including response to endoplasmic reticulum stress (ER stress), which is produced by the accumulation of unfolded proteins in the lume...
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| Format: | Article |
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MDPI AG
2019-07-01
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| Series: | Cells |
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| Online Access: | https://www.mdpi.com/2073-4409/8/7/710 |
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| author | Mariana Hernández-Elvira Ricardo Martínez-Gómez Eunice Domínguez-Martin Akram Méndez Laura Kawasaki Laura Ongay-Larios Roberto Coria |
| author_facet | Mariana Hernández-Elvira Ricardo Martínez-Gómez Eunice Domínguez-Martin Akram Méndez Laura Kawasaki Laura Ongay-Larios Roberto Coria |
| author_sort | Mariana Hernández-Elvira |
| collection | DOAJ |
| description | In the yeast <i>Saccharomyces cerevisiae</i>, components of the High Osmolarity Glycerol (HOG) pathway are important for the response to diverse stresses including response to endoplasmic reticulum stress (ER stress), which is produced by the accumulation of unfolded proteins in the lumen of this organelle. Accumulation of unfolded proteins may be due to the inhibition of protein <i>N</i>-glycosylation, which can be achieved by treatment with the antibiotic tunicamycin (Tn). In this work we were interested in finding proteins involved in the ER stress response regulated by Hog1, the mitogen activated protein kinase (MAPK) of the HOG pathway. A high gene dosage suppression screening allowed us to identify genes that suppressed the sensitivity to Tn shown by a <i>hog1Δ</i> mutant. The suppressors participate in a limited number of cellular processes, including lipid/carbohydrate biosynthesis and protein glycosylation, vesicle-mediated transport and exocytosis, cell wall organization and biogenesis, and cell detoxification processes. The finding of suppressors Rer2 and Srt1, which participate in the dolichol biosynthesis pathway revealed that the <i>hog1Δ</i> strain has a defective polyprenol metabolism. This work uncovers new genetic and functional interactors of Hog1 and contributes to a better understanding of the participation of this MAPK in the ER stress response. |
| first_indexed | 2024-03-12T09:52:32Z |
| format | Article |
| id | doaj.art-c05c8fc3cb344933935958116272c241 |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-12T09:52:32Z |
| publishDate | 2019-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-c05c8fc3cb344933935958116272c2412023-09-02T12:28:17ZengMDPI AGCells2073-44092019-07-018771010.3390/cells8070710cells8070710Tunicamycin Sensitivity-Suppression by High Gene Dosage Reveals New Functions of the Yeast Hog1 MAP KinaseMariana Hernández-Elvira0Ricardo Martínez-Gómez1Eunice Domínguez-Martin2Akram Méndez3Laura Kawasaki4Laura Ongay-Larios5Roberto Coria6Departamento de Genética Molecular, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, CP 04510 Cd., MexicoDepartamento de Genética Molecular, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, CP 04510 Cd., MexicoDepartamento de Genética Molecular, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, CP 04510 Cd., MexicoDepartamento de Genética Molecular, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, CP 04510 Cd., MexicoDepartamento de Genética Molecular, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, CP 04510 Cd., MexicoUnidad de Biología Molecular, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, CP 04510 Cd., MexicoDepartamento de Genética Molecular, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, CP 04510 Cd., MexicoIn the yeast <i>Saccharomyces cerevisiae</i>, components of the High Osmolarity Glycerol (HOG) pathway are important for the response to diverse stresses including response to endoplasmic reticulum stress (ER stress), which is produced by the accumulation of unfolded proteins in the lumen of this organelle. Accumulation of unfolded proteins may be due to the inhibition of protein <i>N</i>-glycosylation, which can be achieved by treatment with the antibiotic tunicamycin (Tn). In this work we were interested in finding proteins involved in the ER stress response regulated by Hog1, the mitogen activated protein kinase (MAPK) of the HOG pathway. A high gene dosage suppression screening allowed us to identify genes that suppressed the sensitivity to Tn shown by a <i>hog1Δ</i> mutant. The suppressors participate in a limited number of cellular processes, including lipid/carbohydrate biosynthesis and protein glycosylation, vesicle-mediated transport and exocytosis, cell wall organization and biogenesis, and cell detoxification processes. The finding of suppressors Rer2 and Srt1, which participate in the dolichol biosynthesis pathway revealed that the <i>hog1Δ</i> strain has a defective polyprenol metabolism. This work uncovers new genetic and functional interactors of Hog1 and contributes to a better understanding of the participation of this MAPK in the ER stress response.https://www.mdpi.com/2073-4409/8/7/710yeastHog1MAP kinaseendoplasmic reticulumstresssuppressionunfolded protein response (UPR) |
| spellingShingle | Mariana Hernández-Elvira Ricardo Martínez-Gómez Eunice Domínguez-Martin Akram Méndez Laura Kawasaki Laura Ongay-Larios Roberto Coria Tunicamycin Sensitivity-Suppression by High Gene Dosage Reveals New Functions of the Yeast Hog1 MAP Kinase Cells yeast Hog1 MAP kinase endoplasmic reticulum stress suppression unfolded protein response (UPR) |
| title | Tunicamycin Sensitivity-Suppression by High Gene Dosage Reveals New Functions of the Yeast Hog1 MAP Kinase |
| title_full | Tunicamycin Sensitivity-Suppression by High Gene Dosage Reveals New Functions of the Yeast Hog1 MAP Kinase |
| title_fullStr | Tunicamycin Sensitivity-Suppression by High Gene Dosage Reveals New Functions of the Yeast Hog1 MAP Kinase |
| title_full_unstemmed | Tunicamycin Sensitivity-Suppression by High Gene Dosage Reveals New Functions of the Yeast Hog1 MAP Kinase |
| title_short | Tunicamycin Sensitivity-Suppression by High Gene Dosage Reveals New Functions of the Yeast Hog1 MAP Kinase |
| title_sort | tunicamycin sensitivity suppression by high gene dosage reveals new functions of the yeast hog1 map kinase |
| topic | yeast Hog1 MAP kinase endoplasmic reticulum stress suppression unfolded protein response (UPR) |
| url | https://www.mdpi.com/2073-4409/8/7/710 |
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