Evaluation of Lactobacillus brevis MG000874 in Behavioral and In Vitro Antioxidant Enzyme Activity of Murine Brain
Abstract Aging and oxidative stress are important biological processes with extensive medical and social significance. The D-Galactose (D-Gal) has been used in animals for inducing oxidative stress and brain aging. The present study was conducted to evaluate the role of Lactobacillus brevis MG000874...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Instituto de Tecnologia do Paraná (Tecpar)
2022-05-01
|
| Series: | Brazilian Archives of Biology and Technology |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132022000100317&lng=en&tlng=en |
| _version_ | 1818212874518003712 |
|---|---|
| author | Saleha Noureen Iram Liaqat Ayesha Riaz Mehwish Rana Najma Arshad |
| author_facet | Saleha Noureen Iram Liaqat Ayesha Riaz Mehwish Rana Najma Arshad |
| author_sort | Saleha Noureen |
| collection | DOAJ |
| description | Abstract Aging and oxidative stress are important biological processes with extensive medical and social significance. The D-Galactose (D-Gal) has been used in animals for inducing oxidative stress and brain aging. The present study was conducted to evaluate the role of Lactobacillus brevis MG000874 in inhibiting the aging process and oxidative stress in D-Gal induced oxidative stress murine model. The cognitive responses and biochemical indicators were used to estimate the anti-aging and antioxidant potential of L. brevis MG000874 in mice. Tests used to assess cognitive responses, included object recognition, open field and T Maze. While antioxidant status was assessed by measuring superoxide dismutase (SOD), catalase (CAT), glutathione (GSH) and glutathione-S-transferase (GST) activity in mice brain tissues. Results suggested that D-Gal can seriously impair the object recognition in mice. It led to significant reduction in ambulation, rearing, leaning and grooming. Similar trend was observed in antioxidant enzymes such as SOD, CAT and GSH except for GST where elevation was observed. Treatment with L. brevis MG000874 and ascorbic acid inhibited this reduction and kept levels close to control. Likewise, values of GSH of probiotic group were lower than negative control but significantly higher than positive control group. Probiotic supplementation in the presence of D-Gal displayed improvement in GSH, while no such improvement could be observed with ascorbic acid treatment. It can be concluded from the present study that L. brevis MG000874 can be used as anti-aging and antioxidant agent. It is recommended that L. brevis MG000874 could be included in the list of probiotics. |
| first_indexed | 2024-12-12T05:55:20Z |
| format | Article |
| id | doaj.art-c05e943d34a249dc95faeb035bfe62d8 |
| institution | Directory Open Access Journal |
| issn | 1678-4324 |
| language | English |
| last_indexed | 2024-12-12T05:55:20Z |
| publishDate | 2022-05-01 |
| publisher | Instituto de Tecnologia do Paraná (Tecpar) |
| record_format | Article |
| series | Brazilian Archives of Biology and Technology |
| spelling | doaj.art-c05e943d34a249dc95faeb035bfe62d82022-12-22T00:35:34ZengInstituto de Tecnologia do Paraná (Tecpar)Brazilian Archives of Biology and Technology1678-43242022-05-016510.1590/1678-4324-2022210294Evaluation of Lactobacillus brevis MG000874 in Behavioral and In Vitro Antioxidant Enzyme Activity of Murine BrainSaleha Noureenhttps://orcid.org/0000-0002-8069-8634Iram Liaqathttps://orcid.org/0000-0002-4638-8253Ayesha Riazhttps://orcid.org/0000-0003-1868-2073Mehwish Ranahttps://orcid.org/0000-0002-2653-4671Najma Arshadhttps://orcid.org/0000-0002-0244-6757Abstract Aging and oxidative stress are important biological processes with extensive medical and social significance. The D-Galactose (D-Gal) has been used in animals for inducing oxidative stress and brain aging. The present study was conducted to evaluate the role of Lactobacillus brevis MG000874 in inhibiting the aging process and oxidative stress in D-Gal induced oxidative stress murine model. The cognitive responses and biochemical indicators were used to estimate the anti-aging and antioxidant potential of L. brevis MG000874 in mice. Tests used to assess cognitive responses, included object recognition, open field and T Maze. While antioxidant status was assessed by measuring superoxide dismutase (SOD), catalase (CAT), glutathione (GSH) and glutathione-S-transferase (GST) activity in mice brain tissues. Results suggested that D-Gal can seriously impair the object recognition in mice. It led to significant reduction in ambulation, rearing, leaning and grooming. Similar trend was observed in antioxidant enzymes such as SOD, CAT and GSH except for GST where elevation was observed. Treatment with L. brevis MG000874 and ascorbic acid inhibited this reduction and kept levels close to control. Likewise, values of GSH of probiotic group were lower than negative control but significantly higher than positive control group. Probiotic supplementation in the presence of D-Gal displayed improvement in GSH, while no such improvement could be observed with ascorbic acid treatment. It can be concluded from the present study that L. brevis MG000874 can be used as anti-aging and antioxidant agent. It is recommended that L. brevis MG000874 could be included in the list of probiotics.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132022000100317&lng=en&tlng=enL. brevis MG000874D-Galactoseanti-aging and antioxidant potentialprobiotics |
| spellingShingle | Saleha Noureen Iram Liaqat Ayesha Riaz Mehwish Rana Najma Arshad Evaluation of Lactobacillus brevis MG000874 in Behavioral and In Vitro Antioxidant Enzyme Activity of Murine Brain Brazilian Archives of Biology and Technology L. brevis MG000874 D-Galactose anti-aging and antioxidant potential probiotics |
| title | Evaluation of Lactobacillus brevis MG000874 in Behavioral and In Vitro Antioxidant Enzyme Activity of Murine Brain |
| title_full | Evaluation of Lactobacillus brevis MG000874 in Behavioral and In Vitro Antioxidant Enzyme Activity of Murine Brain |
| title_fullStr | Evaluation of Lactobacillus brevis MG000874 in Behavioral and In Vitro Antioxidant Enzyme Activity of Murine Brain |
| title_full_unstemmed | Evaluation of Lactobacillus brevis MG000874 in Behavioral and In Vitro Antioxidant Enzyme Activity of Murine Brain |
| title_short | Evaluation of Lactobacillus brevis MG000874 in Behavioral and In Vitro Antioxidant Enzyme Activity of Murine Brain |
| title_sort | evaluation of lactobacillus brevis mg000874 in behavioral and in vitro antioxidant enzyme activity of murine brain |
| topic | L. brevis MG000874 D-Galactose anti-aging and antioxidant potential probiotics |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132022000100317&lng=en&tlng=en |
| work_keys_str_mv | AT salehanoureen evaluationoflactobacillusbrevismg000874inbehavioralandinvitroantioxidantenzymeactivityofmurinebrain AT iramliaqat evaluationoflactobacillusbrevismg000874inbehavioralandinvitroantioxidantenzymeactivityofmurinebrain AT ayeshariaz evaluationoflactobacillusbrevismg000874inbehavioralandinvitroantioxidantenzymeactivityofmurinebrain AT mehwishrana evaluationoflactobacillusbrevismg000874inbehavioralandinvitroantioxidantenzymeactivityofmurinebrain AT najmaarshad evaluationoflactobacillusbrevismg000874inbehavioralandinvitroantioxidantenzymeactivityofmurinebrain |