SUV39H1 is a novel biomarker targeting oxidative phosphorylation in hepatitis B virus-associated hepatocellular carcinoma
Abstract Background As a histone methyltransferase, suppressor of variegation 3–9 homolog 1 (SUV39H1) plays an important role in the occurrence and development of cancer. To explore the mechanism and biological function of SUV39H1 in hepatitis B virus-associated hepatocellular carcinoma (HBV-HCC) ca...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2023-11-01
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| Series: | BMC Cancer |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12885-023-11633-4 |
| _version_ | 1797414819932930048 |
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| author | Yanping Zhang Wanwen Lao Kaming Yang Xinyi Kong Yuetong Li Xin Yu Xumeng Wang Yang Liu Zhenlin Li Yilin Deng Shuping Nie Changlong Bi Chao Wu Aixia Zhai |
| author_facet | Yanping Zhang Wanwen Lao Kaming Yang Xinyi Kong Yuetong Li Xin Yu Xumeng Wang Yang Liu Zhenlin Li Yilin Deng Shuping Nie Changlong Bi Chao Wu Aixia Zhai |
| author_sort | Yanping Zhang |
| collection | DOAJ |
| description | Abstract Background As a histone methyltransferase, suppressor of variegation 3–9 homolog 1 (SUV39H1) plays an important role in the occurrence and development of cancer. To explore the mechanism and biological function of SUV39H1 in hepatitis B virus-associated hepatocellular carcinoma (HBV-HCC) can gain an insight into the pathogenesis of HBV-HCC. Methods The effect of HBV infection on SUV39H1 in hepatoma cells was detected. CCK-8, colony growth assay and wound healing assay were used to assess the proliferation and migration of HBV-positive hepatoma cells. RNA sequencing (RNA-seq) was applied to find differential genes and enriched pathways. The serum SUV39H1 level in HBV-HCC patients was detected and its correlation with clinical indicators was analyzed. Results SUV39H1 was increased by HBV infection and promoted the proliferation and migration of hepatoma cells. SUV39H1 could upregulate the expression of mitochondrial oxidative phosphorylation (OXPHOS) pathway-related genes. OXPHOS pathway inhibitors could reduce the capacity of proliferation and migration of hepatoma cells after overexpressing SUV39H1. Serum SUV39H1 levels were higher in chronic hepatitis B (CHB) patients than in healthy controls and higher in HBV-HCC patients than in CHB patients. In the diagnosis of HCC, the predictive value of SUV39H1 combined with alpha-fetoprotein (AFP) was better than that of AFP alone. Conclusion SUV39H1 is regulated by HBV infection and promotes the proliferation and migration of hepatoma cells by targeting OXPHOS pathway. It indicates that SUV39H1 may be a new biomarker of the diagnosis of HCC. |
| first_indexed | 2024-03-09T05:39:30Z |
| format | Article |
| id | doaj.art-c05f3d2b70864dc593b1028782fe4af2 |
| institution | Directory Open Access Journal |
| issn | 1471-2407 |
| language | English |
| last_indexed | 2024-03-09T05:39:30Z |
| publishDate | 2023-11-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Cancer |
| spelling | doaj.art-c05f3d2b70864dc593b1028782fe4af22023-12-03T12:26:12ZengBMCBMC Cancer1471-24072023-11-0123111310.1186/s12885-023-11633-4SUV39H1 is a novel biomarker targeting oxidative phosphorylation in hepatitis B virus-associated hepatocellular carcinomaYanping Zhang0Wanwen Lao1Kaming Yang2Xinyi Kong3Yuetong Li4Xin Yu5Xumeng Wang6Yang Liu7Zhenlin Li8Yilin Deng9Shuping Nie10Changlong Bi11Chao Wu12Aixia Zhai13Department of Laboratory Medicine, The Eighth Affiliated Hospital, Sun Yat-sen UniversityDepartment of Endocrinology, The Eighth Affiliated Hospital, Sun Yat-sen UniversityDepartment of Endocrinology, The Eighth Affiliated Hospital, Sun Yat-sen UniversityDepartment of Laboratory Medicine, The Eighth Affiliated Hospital, Sun Yat-sen UniversityDepartment of Endocrinology, The Eighth Affiliated Hospital, Sun Yat-sen UniversityDepartment of Laboratory Medicine, The Eighth Affiliated Hospital, Sun Yat-sen UniversityDepartment of Laboratory Medicine, The Eighth Affiliated Hospital, Sun Yat-sen UniversityDepartment of Laboratory Medicine, The Eighth Affiliated Hospital, Sun Yat-sen UniversityDepartment of Endocrinology, The Eighth Affiliated Hospital, Sun Yat-sen UniversityDepartment of Laboratory Medicine, The Eighth Affiliated Hospital, Sun Yat-sen UniversityDepartment of Laboratory Medicine, The Eighth Affiliated Hospital, Sun Yat-sen UniversityDepartment of Endocrinology, The Eighth Affiliated Hospital, Sun Yat-sen UniversityDepartment of Laboratory Medicine, The Eighth Affiliated Hospital, Sun Yat-sen UniversityDepartment of Laboratory Medicine, The Eighth Affiliated Hospital, Sun Yat-sen UniversityAbstract Background As a histone methyltransferase, suppressor of variegation 3–9 homolog 1 (SUV39H1) plays an important role in the occurrence and development of cancer. To explore the mechanism and biological function of SUV39H1 in hepatitis B virus-associated hepatocellular carcinoma (HBV-HCC) can gain an insight into the pathogenesis of HBV-HCC. Methods The effect of HBV infection on SUV39H1 in hepatoma cells was detected. CCK-8, colony growth assay and wound healing assay were used to assess the proliferation and migration of HBV-positive hepatoma cells. RNA sequencing (RNA-seq) was applied to find differential genes and enriched pathways. The serum SUV39H1 level in HBV-HCC patients was detected and its correlation with clinical indicators was analyzed. Results SUV39H1 was increased by HBV infection and promoted the proliferation and migration of hepatoma cells. SUV39H1 could upregulate the expression of mitochondrial oxidative phosphorylation (OXPHOS) pathway-related genes. OXPHOS pathway inhibitors could reduce the capacity of proliferation and migration of hepatoma cells after overexpressing SUV39H1. Serum SUV39H1 levels were higher in chronic hepatitis B (CHB) patients than in healthy controls and higher in HBV-HCC patients than in CHB patients. In the diagnosis of HCC, the predictive value of SUV39H1 combined with alpha-fetoprotein (AFP) was better than that of AFP alone. Conclusion SUV39H1 is regulated by HBV infection and promotes the proliferation and migration of hepatoma cells by targeting OXPHOS pathway. It indicates that SUV39H1 may be a new biomarker of the diagnosis of HCC.https://doi.org/10.1186/s12885-023-11633-4SUV39H1Hepatocellular carcinomaHepatitis B virusOxidative phosphorylationBiomarker |
| spellingShingle | Yanping Zhang Wanwen Lao Kaming Yang Xinyi Kong Yuetong Li Xin Yu Xumeng Wang Yang Liu Zhenlin Li Yilin Deng Shuping Nie Changlong Bi Chao Wu Aixia Zhai SUV39H1 is a novel biomarker targeting oxidative phosphorylation in hepatitis B virus-associated hepatocellular carcinoma BMC Cancer SUV39H1 Hepatocellular carcinoma Hepatitis B virus Oxidative phosphorylation Biomarker |
| title | SUV39H1 is a novel biomarker targeting oxidative phosphorylation in hepatitis B virus-associated hepatocellular carcinoma |
| title_full | SUV39H1 is a novel biomarker targeting oxidative phosphorylation in hepatitis B virus-associated hepatocellular carcinoma |
| title_fullStr | SUV39H1 is a novel biomarker targeting oxidative phosphorylation in hepatitis B virus-associated hepatocellular carcinoma |
| title_full_unstemmed | SUV39H1 is a novel biomarker targeting oxidative phosphorylation in hepatitis B virus-associated hepatocellular carcinoma |
| title_short | SUV39H1 is a novel biomarker targeting oxidative phosphorylation in hepatitis B virus-associated hepatocellular carcinoma |
| title_sort | suv39h1 is a novel biomarker targeting oxidative phosphorylation in hepatitis b virus associated hepatocellular carcinoma |
| topic | SUV39H1 Hepatocellular carcinoma Hepatitis B virus Oxidative phosphorylation Biomarker |
| url | https://doi.org/10.1186/s12885-023-11633-4 |
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