Natural silk fiber microcarriers for cell culture
The development of effective and versatile microcarriers is a pressing issue in tissue engineering and regenerative medicine. The objective of this work is to create biocompatible fiber microparticles from the cocoons of the Bombyx mori silkworm, and to study their structure and biological properti...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | Russian |
| Published: |
Federal Research Center of Transplantology and Artificial Organs named after V.I.Shumakov
2021-01-01
|
| Series: | Vestnik Transplantologii i Iskusstvennyh Organov |
| Subjects: | |
| Online Access: | https://journal.transpl.ru/vtio/article/view/1270 |
| _version_ | 1797871955611746304 |
|---|---|
| author | M. M. Bobrova L. A. Safonova A. E. Efimov O. I. Agapova I. I. Agapov |
| author_facet | M. M. Bobrova L. A. Safonova A. E. Efimov O. I. Agapova I. I. Agapov |
| author_sort | M. M. Bobrova |
| collection | DOAJ |
| description | The development of effective and versatile microcarriers is a pressing issue in tissue engineering and regenerative medicine. The objective of this work is to create biocompatible fiber microparticles from the cocoons of the Bombyx mori silkworm, and to study their structure and biological properties. Materials and methods. In obtaining microparticles, the Bombyx mori cocoons washed from sericin were cryo-milled in liquid nitrogen. The structure of the resulting microparticles was analyzed via scanning electron microscopy. The cytotoxicity of the obtained fibers was assessed using MTT-cell culture assay of 3T3 mouse fibroblasts. Cell adhesion analysis was performed using the Hep-G2 human hepatocarcinoma cell line. Cell visualization was performed by staining the nuclei with DAPI fluorescent dye. Results. Natural silk microparticles were obtained in the form of cylindrical fibers with 200–400 μm average length and 15 μm diameter. It was shown that the surface of the resulting microparticles has a rough relief; no pores were found. The microparticles are non-toxic for 3T3 mouse fibroblasts, they maintain a high level of adhesion by human hepatocellular carcinoma HepG2 cells. Conclusion. The method developed by us for fabrication of biocompatible silk fibroin microparticles in the form of fibers without using toxic reagents and significant time costs is promising for cell cultivation and delivery to the damaged area for tissue and organ regeneration. |
| first_indexed | 2024-04-10T00:51:09Z |
| format | Article |
| id | doaj.art-c0601d3447fe41f191b59f9a49968a4b |
| institution | Directory Open Access Journal |
| issn | 1995-1191 |
| language | Russian |
| last_indexed | 2024-04-10T00:51:09Z |
| publishDate | 2021-01-01 |
| publisher | Federal Research Center of Transplantology and Artificial Organs named after V.I.Shumakov |
| record_format | Article |
| series | Vestnik Transplantologii i Iskusstvennyh Organov |
| spelling | doaj.art-c0601d3447fe41f191b59f9a49968a4b2023-03-13T10:37:27ZrusFederal Research Center of Transplantology and Artificial Organs named after V.I.ShumakovVestnik Transplantologii i Iskusstvennyh Organov1995-11912021-01-012249810410.15825/1995-1191-2020-4-98-104925Natural silk fiber microcarriers for cell cultureM. M. Bobrova0L. A. Safonova1A. E. Efimov2O. I. Agapova3I. I. Agapov4Shumakov National Medical Research Center of Transplantology and Artificial OrgansShumakov National Medical Research Center of Transplantology and Artificial OrgansShumakov National Medical Research Center of Transplantology and Artificial OrgansShumakov National Medical Research Center of Transplantology and Artificial OrgansShumakov National Medical Research Center of Transplantology and Artificial OrgansThe development of effective and versatile microcarriers is a pressing issue in tissue engineering and regenerative medicine. The objective of this work is to create biocompatible fiber microparticles from the cocoons of the Bombyx mori silkworm, and to study their structure and biological properties. Materials and methods. In obtaining microparticles, the Bombyx mori cocoons washed from sericin were cryo-milled in liquid nitrogen. The structure of the resulting microparticles was analyzed via scanning electron microscopy. The cytotoxicity of the obtained fibers was assessed using MTT-cell culture assay of 3T3 mouse fibroblasts. Cell adhesion analysis was performed using the Hep-G2 human hepatocarcinoma cell line. Cell visualization was performed by staining the nuclei with DAPI fluorescent dye. Results. Natural silk microparticles were obtained in the form of cylindrical fibers with 200–400 μm average length and 15 μm diameter. It was shown that the surface of the resulting microparticles has a rough relief; no pores were found. The microparticles are non-toxic for 3T3 mouse fibroblasts, they maintain a high level of adhesion by human hepatocellular carcinoma HepG2 cells. Conclusion. The method developed by us for fabrication of biocompatible silk fibroin microparticles in the form of fibers without using toxic reagents and significant time costs is promising for cell cultivation and delivery to the damaged area for tissue and organ regeneration.https://journal.transpl.ru/vtio/article/view/1270microcarrierssilk fibroinfibers |
| spellingShingle | M. M. Bobrova L. A. Safonova A. E. Efimov O. I. Agapova I. I. Agapov Natural silk fiber microcarriers for cell culture Vestnik Transplantologii i Iskusstvennyh Organov microcarriers silk fibroin fibers |
| title | Natural silk fiber microcarriers for cell culture |
| title_full | Natural silk fiber microcarriers for cell culture |
| title_fullStr | Natural silk fiber microcarriers for cell culture |
| title_full_unstemmed | Natural silk fiber microcarriers for cell culture |
| title_short | Natural silk fiber microcarriers for cell culture |
| title_sort | natural silk fiber microcarriers for cell culture |
| topic | microcarriers silk fibroin fibers |
| url | https://journal.transpl.ru/vtio/article/view/1270 |
| work_keys_str_mv | AT mmbobrova naturalsilkfibermicrocarriersforcellculture AT lasafonova naturalsilkfibermicrocarriersforcellculture AT aeefimov naturalsilkfibermicrocarriersforcellculture AT oiagapova naturalsilkfibermicrocarriersforcellculture AT iiagapov naturalsilkfibermicrocarriersforcellculture |