Detection of multidrug resistance Aeromonas hydrophila in farm raised fresh water prawns
This study was undertaken for isolation, identification and determination of antibiogram profile of Aeromonas hydrophila in farm raised fresh water prawn (Macrobrachium rosenbergii) on five commercial ghers. Fresh water prawns (n=25) were collected from five ghers located at Satkhira, Bagerhat and K...
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| Format: | Article |
| Language: | English |
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Network for the Veterinarians of Bangladesh
2015-12-01
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| Series: | Journal of Advanced Veterinary and Animal Research |
| Subjects: | |
| Online Access: | http://doi.org/10.5455/javar.2015.b120 |
| _version_ | 1831704227548758016 |
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| author | Md. Bakhtiar Lijon Mst. Mousumi Khatun Ariful Islam Mst. Minara Khatun Md. Ariful Islam |
| author_facet | Md. Bakhtiar Lijon Mst. Mousumi Khatun Ariful Islam Mst. Minara Khatun Md. Ariful Islam |
| author_sort | Md. Bakhtiar Lijon |
| collection | DOAJ |
| description | This study was undertaken for isolation, identification and determination of antibiogram profile of Aeromonas hydrophila in farm raised fresh water prawn (Macrobrachium rosenbergii) on five commercial ghers. Fresh water prawns (n=25) were collected from five ghers located at Satkhira, Bagerhat and Khulna districts of Bangladesh. Brain (n=25), muscle (n=25) and intestine (n=25) samples were collected aseptically from fresh water prawn and inoculated into alkaline peptone (APW) water for enrichment at 37℃ for 8 h. Enriched cultured was streaked into Thiosulfate Citrate Bile Salt Sucrose (TCBS) agar to isolate bacteria. Bacteria were identified by cultural, Gram staining, biochemical properties and polymerase chain reaction (PCR) assay. The antibiogram profiles of bacteria were investigated against 5 commonly used antibiotics (Gentamicin, Cefalexin, Ampicillin, Azithromycin and Ciprofloxacin) by disc diffusion method. Thirteen A. hydrophila isolates were identified and the prevalence of the A. hydrophila in fresh water prawn was 17.33%. All 13 (100%) isolates were sensitive to Ciprofloxacin, Gentamicin, Azithromycin and resistant to Ampicillin and Cefalexin. The results of this study indicate that farm raised fresh water prawn harbor multidrug resistant A. hydrophila which might causes public health problem if enter into human food chain. |
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| institution | Directory Open Access Journal |
| issn | 2311-7710 2311-7710 |
| language | English |
| last_indexed | 2024-12-20T15:55:17Z |
| publishDate | 2015-12-01 |
| publisher | Network for the Veterinarians of Bangladesh |
| record_format | Article |
| series | Journal of Advanced Veterinary and Animal Research |
| spelling | doaj.art-c0613bc68a2d4bb6894ef7ca959f2a922022-12-21T19:34:31ZengNetwork for the Veterinarians of BangladeshJournal of Advanced Veterinary and Animal Research2311-77102311-77102015-12-012446947410.5455/javar.2015.b120Detection of multidrug resistance Aeromonas hydrophila in farm raised fresh water prawnsMd. Bakhtiar Lijon0Mst. Mousumi Khatun1Ariful Islam2Mst. Minara Khatun 3Md. Ariful Islam4Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh-2202, BangladeshDepartment of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh-2202, BangladeshDepartment of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh-2202, BangladeshDepartment of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh-2202, BangladeshDepartment of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh-2202, BangladeshThis study was undertaken for isolation, identification and determination of antibiogram profile of Aeromonas hydrophila in farm raised fresh water prawn (Macrobrachium rosenbergii) on five commercial ghers. Fresh water prawns (n=25) were collected from five ghers located at Satkhira, Bagerhat and Khulna districts of Bangladesh. Brain (n=25), muscle (n=25) and intestine (n=25) samples were collected aseptically from fresh water prawn and inoculated into alkaline peptone (APW) water for enrichment at 37℃ for 8 h. Enriched cultured was streaked into Thiosulfate Citrate Bile Salt Sucrose (TCBS) agar to isolate bacteria. Bacteria were identified by cultural, Gram staining, biochemical properties and polymerase chain reaction (PCR) assay. The antibiogram profiles of bacteria were investigated against 5 commonly used antibiotics (Gentamicin, Cefalexin, Ampicillin, Azithromycin and Ciprofloxacin) by disc diffusion method. Thirteen A. hydrophila isolates were identified and the prevalence of the A. hydrophila in fresh water prawn was 17.33%. All 13 (100%) isolates were sensitive to Ciprofloxacin, Gentamicin, Azithromycin and resistant to Ampicillin and Cefalexin. The results of this study indicate that farm raised fresh water prawn harbor multidrug resistant A. hydrophila which might causes public health problem if enter into human food chain.http://doi.org/10.5455/javar.2015.b120Aeromonas hydrophilaAntibiogram profileFresh water prawnPrevalencePCR assay |
| spellingShingle | Md. Bakhtiar Lijon Mst. Mousumi Khatun Ariful Islam Mst. Minara Khatun Md. Ariful Islam Detection of multidrug resistance Aeromonas hydrophila in farm raised fresh water prawns Journal of Advanced Veterinary and Animal Research Aeromonas hydrophila Antibiogram profile Fresh water prawn Prevalence PCR assay |
| title | Detection of multidrug resistance Aeromonas hydrophila in farm raised fresh water prawns |
| title_full | Detection of multidrug resistance Aeromonas hydrophila in farm raised fresh water prawns |
| title_fullStr | Detection of multidrug resistance Aeromonas hydrophila in farm raised fresh water prawns |
| title_full_unstemmed | Detection of multidrug resistance Aeromonas hydrophila in farm raised fresh water prawns |
| title_short | Detection of multidrug resistance Aeromonas hydrophila in farm raised fresh water prawns |
| title_sort | detection of multidrug resistance aeromonas hydrophila in farm raised fresh water prawns |
| topic | Aeromonas hydrophila Antibiogram profile Fresh water prawn Prevalence PCR assay |
| url | http://doi.org/10.5455/javar.2015.b120 |
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