Gene Expression Profiling in Abdominal Aortic Aneurysms
Gene expression profiling of abdominal aortic aneurysms (AAA) indicates that chronic inflammatory responses, active matrix metalloproteinases, and degradation of the extracellular matrix components are involved in disease development and progression. This study investigates intra- and interpersonal...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-06-01
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| Series: | Journal of Clinical Medicine |
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| Online Access: | https://www.mdpi.com/2077-0383/11/12/3260 |
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| author | Amelie L. Behrens Susanne Dihlmann Caspar Grond-Ginsbach Andreas S. Peters Bernhard Dorweiler Dittmar Böckler Philipp Erhart |
| author_facet | Amelie L. Behrens Susanne Dihlmann Caspar Grond-Ginsbach Andreas S. Peters Bernhard Dorweiler Dittmar Böckler Philipp Erhart |
| author_sort | Amelie L. Behrens |
| collection | DOAJ |
| description | Gene expression profiling of abdominal aortic aneurysms (AAA) indicates that chronic inflammatory responses, active matrix metalloproteinases, and degradation of the extracellular matrix components are involved in disease development and progression. This study investigates intra- and interpersonal RNA genome-wide expression profiling differences (Illumina HumanHT-12, BeadCHIP expression) of 24 AAA biopsies from 12 patients using a single gene and pathway (GeneOntology, GO enrichment) analysis. Biopsies were collected during open surgical AAA repair and according to prior finite element analysis (FEA) from regions with the highest and lowest wall stress. Single gene analysis revealed a strong heterogeneity of RNA expression parameters within the same and different AAA biopsies. The pathway analysis of all samples showed significant enrichment of genes from three different signaling pathways (integrin signaling pathway: fold change FC 1.63, <i>p</i> = 0.001; cholecystokinin receptor pathway: FC 1.60, <i>p</i> = 0.011; inflammation mediated by chemokine signaling pathway: FC 1.45, <i>p</i> = 0.028). These results indicate heterogeneous gene expression patterns within the AAA vascular wall. Single biopsy investigations do not permit a comprehensive characterization of activated molecular processes in AAA disease. |
| first_indexed | 2024-03-09T23:29:35Z |
| format | Article |
| id | doaj.art-c06156e4c3b546f09de0505970020078 |
| institution | Directory Open Access Journal |
| issn | 2077-0383 |
| language | English |
| last_indexed | 2024-03-09T23:29:35Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Clinical Medicine |
| spelling | doaj.art-c06156e4c3b546f09de05059700200782023-11-23T17:13:01ZengMDPI AGJournal of Clinical Medicine2077-03832022-06-011112326010.3390/jcm11123260Gene Expression Profiling in Abdominal Aortic AneurysmsAmelie L. Behrens0Susanne Dihlmann1Caspar Grond-Ginsbach2Andreas S. Peters3Bernhard Dorweiler4Dittmar Böckler5Philipp Erhart6Department of Vascular and Endovascular Surgery, University Hospital of Heidelberg, 69120 Heidelberg, GermanyDepartment of Vascular and Endovascular Surgery, University Hospital of Heidelberg, 69120 Heidelberg, GermanyDepartment of Vascular and Endovascular Surgery, University Hospital of Heidelberg, 69120 Heidelberg, GermanyDepartment of Vascular and Endovascular Surgery, University Hospital of Heidelberg, 69120 Heidelberg, GermanyDepartment of Vascular and Endovascular Surgery, University Hospital Cologne, 50937 Köln, GermanyDepartment of Vascular and Endovascular Surgery, University Hospital of Heidelberg, 69120 Heidelberg, GermanyDepartment of Vascular and Endovascular Surgery, University Hospital of Heidelberg, 69120 Heidelberg, GermanyGene expression profiling of abdominal aortic aneurysms (AAA) indicates that chronic inflammatory responses, active matrix metalloproteinases, and degradation of the extracellular matrix components are involved in disease development and progression. This study investigates intra- and interpersonal RNA genome-wide expression profiling differences (Illumina HumanHT-12, BeadCHIP expression) of 24 AAA biopsies from 12 patients using a single gene and pathway (GeneOntology, GO enrichment) analysis. Biopsies were collected during open surgical AAA repair and according to prior finite element analysis (FEA) from regions with the highest and lowest wall stress. Single gene analysis revealed a strong heterogeneity of RNA expression parameters within the same and different AAA biopsies. The pathway analysis of all samples showed significant enrichment of genes from three different signaling pathways (integrin signaling pathway: fold change FC 1.63, <i>p</i> = 0.001; cholecystokinin receptor pathway: FC 1.60, <i>p</i> = 0.011; inflammation mediated by chemokine signaling pathway: FC 1.45, <i>p</i> = 0.028). These results indicate heterogeneous gene expression patterns within the AAA vascular wall. Single biopsy investigations do not permit a comprehensive characterization of activated molecular processes in AAA disease.https://www.mdpi.com/2077-0383/11/12/3260abdominal aortic aneurysmgene expressionmechanotransductionrupture risk |
| spellingShingle | Amelie L. Behrens Susanne Dihlmann Caspar Grond-Ginsbach Andreas S. Peters Bernhard Dorweiler Dittmar Böckler Philipp Erhart Gene Expression Profiling in Abdominal Aortic Aneurysms Journal of Clinical Medicine abdominal aortic aneurysm gene expression mechanotransduction rupture risk |
| title | Gene Expression Profiling in Abdominal Aortic Aneurysms |
| title_full | Gene Expression Profiling in Abdominal Aortic Aneurysms |
| title_fullStr | Gene Expression Profiling in Abdominal Aortic Aneurysms |
| title_full_unstemmed | Gene Expression Profiling in Abdominal Aortic Aneurysms |
| title_short | Gene Expression Profiling in Abdominal Aortic Aneurysms |
| title_sort | gene expression profiling in abdominal aortic aneurysms |
| topic | abdominal aortic aneurysm gene expression mechanotransduction rupture risk |
| url | https://www.mdpi.com/2077-0383/11/12/3260 |
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