Tumor growth analysis by magnetic resonance imaging of the C6 glioblastoma model with prospects for the assessment of magnetohyperthermia therapy
OBJECTIVE: The objective was to establish a pattern of tumor growth of the C6 model of glioblastoma multiform in Wistar rats via magnetic resonance imaging (MRI) for the subsequent verification of tumor volume reduction due to magnetic hyperthermia therapy. METHODS: Young male Wistar rats weighing b...
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Instituto Israelita de Ensino e Pesquisa Albert Einstein
2012-03-01
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| Series: | Einstein (São Paulo) |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-45082012000100004&tlng=en |
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| author | André César da Silva Francisco Romero Cabral Javier Bustamante Mamani Jackeline Moraes Malheiros Roberson Saraiva Polli Alberto Tannus Edson Vidoto Mateus José Martins Tatiana Tais Sibov Lorena Favaro Pavon Liza Aya Mabuchi Miyaki Walter Humberto Zavala Cárdenas Suzana Maria Fleury Malheiros Reynaldo André Brandt Edson Amaro Junior Lionel Fernel Gamarra |
| author_facet | André César da Silva Francisco Romero Cabral Javier Bustamante Mamani Jackeline Moraes Malheiros Roberson Saraiva Polli Alberto Tannus Edson Vidoto Mateus José Martins Tatiana Tais Sibov Lorena Favaro Pavon Liza Aya Mabuchi Miyaki Walter Humberto Zavala Cárdenas Suzana Maria Fleury Malheiros Reynaldo André Brandt Edson Amaro Junior Lionel Fernel Gamarra |
| author_sort | André César da Silva |
| collection | DOAJ |
| description | OBJECTIVE: The objective was to establish a pattern of tumor growth of the C6 model of glioblastoma multiform in Wistar rats via magnetic resonance imaging (MRI) for the subsequent verification of tumor volume reduction due to magnetic hyperthermia therapy. METHODS: Young male Wistar rats weighing between 250 and 300 g were used for the C6 model. After the rates were anesthetized (55 mg/kg ketamine and 11 mg/kg xylazine), C6 lineage tumorigenic cells suspended in culture medium (10(5) cells in 10 µl) were stereotaxically injected into the right frontal cortex (bregma coordinates: 2.0 mm anteroposterior, 3.0 mm laterolateral, and 2.5 mm depth) of the rats using a Hamilton syringe. For the control group, the rats were injected with culture medium without cells. MRI scans were performed at 14, 21, and 28 d after the injection using a 2.0 T MRI scanner (Bruker BioSpec, Germany). The animals were anesthetized with 55 mg/kg ketamine and 11 mg/kg xylazine before being examined. Coronal multilayers were acquired using a standard spin echo sequence with the following parameters: repetition/echo time = 4.000 ms/67.1 ms, field of view = 3.50, matrix = 192, slice thickness = 0.4 mm, and slice separation = 0 mm. RESULTS: The MRI analysis enabled a clear visualization of the tumor mass, and it was possible to establish the tumor volume parameters on the various days that were examined. The volume at 14 d after induction was 13.7 ± 2.5 mm³. On days 21 and 28, the tumor volumes were 31.7 ± 6.5 mm³ and 122.1 ± 11.8 mm³, respectively. CONCLUSION: These results demonstrated that it is possible to evaluate the C6 model tumor volume in rats, which will allow for the future implementation and verification of magnetic hyperthermia therapy. |
| first_indexed | 2024-12-24T01:15:21Z |
| format | Article |
| id | doaj.art-c061f647ea914aa3a46b3cddd72a5f5d |
| institution | Directory Open Access Journal |
| issn | 2317-6385 |
| language | English |
| last_indexed | 2024-12-24T01:15:21Z |
| publishDate | 2012-03-01 |
| publisher | Instituto Israelita de Ensino e Pesquisa Albert Einstein |
| record_format | Article |
| series | Einstein (São Paulo) |
| spelling | doaj.art-c061f647ea914aa3a46b3cddd72a5f5d2022-12-21T17:22:47ZengInstituto Israelita de Ensino e Pesquisa Albert EinsteinEinstein (São Paulo)2317-63852012-03-011011115Tumor growth analysis by magnetic resonance imaging of the C6 glioblastoma model with prospects for the assessment of magnetohyperthermia therapyAndré César da Silva0Francisco Romero Cabral1Javier Bustamante Mamani2Jackeline Moraes Malheiros3Roberson Saraiva Polli4Alberto Tannus5Edson Vidoto6Mateus José Martins7Tatiana Tais Sibov8Lorena Favaro Pavon9Liza Aya Mabuchi Miyaki10Walter Humberto Zavala Cárdenas11Suzana Maria Fleury Malheiros12Reynaldo André Brandt13Edson Amaro Junior14Lionel Fernel Gamarra15Hospital Israelita Albert EinsteinHospital Israelita Albert EinsteinHospital Israelita Albert EinsteinUniversidade de São PauloUniversidade de São PauloUniversidade de São PauloUniversidade de São PauloUniversidade de São PauloHospital Israelita Albert EinsteinHospital Israelita Albert EinsteinHospital Israelita Albert EinsteinHospital Israelita Albert EinsteinUniversidade Federal de São PauloSociedade Beneficente Israelita Brasileira Albert EinsteinHospital Israelita Albert EinsteinHospital Israelita Albert EinsteinOBJECTIVE: The objective was to establish a pattern of tumor growth of the C6 model of glioblastoma multiform in Wistar rats via magnetic resonance imaging (MRI) for the subsequent verification of tumor volume reduction due to magnetic hyperthermia therapy. METHODS: Young male Wistar rats weighing between 250 and 300 g were used for the C6 model. After the rates were anesthetized (55 mg/kg ketamine and 11 mg/kg xylazine), C6 lineage tumorigenic cells suspended in culture medium (10(5) cells in 10 µl) were stereotaxically injected into the right frontal cortex (bregma coordinates: 2.0 mm anteroposterior, 3.0 mm laterolateral, and 2.5 mm depth) of the rats using a Hamilton syringe. For the control group, the rats were injected with culture medium without cells. MRI scans were performed at 14, 21, and 28 d after the injection using a 2.0 T MRI scanner (Bruker BioSpec, Germany). The animals were anesthetized with 55 mg/kg ketamine and 11 mg/kg xylazine before being examined. Coronal multilayers were acquired using a standard spin echo sequence with the following parameters: repetition/echo time = 4.000 ms/67.1 ms, field of view = 3.50, matrix = 192, slice thickness = 0.4 mm, and slice separation = 0 mm. RESULTS: The MRI analysis enabled a clear visualization of the tumor mass, and it was possible to establish the tumor volume parameters on the various days that were examined. The volume at 14 d after induction was 13.7 ± 2.5 mm³. On days 21 and 28, the tumor volumes were 31.7 ± 6.5 mm³ and 122.1 ± 11.8 mm³, respectively. CONCLUSION: These results demonstrated that it is possible to evaluate the C6 model tumor volume in rats, which will allow for the future implementation and verification of magnetic hyperthermia therapy.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-45082012000100004&tlng=enMagnetic resonance imagingGlioblastomaBrain neoplasmsRats, Wistar |
| spellingShingle | André César da Silva Francisco Romero Cabral Javier Bustamante Mamani Jackeline Moraes Malheiros Roberson Saraiva Polli Alberto Tannus Edson Vidoto Mateus José Martins Tatiana Tais Sibov Lorena Favaro Pavon Liza Aya Mabuchi Miyaki Walter Humberto Zavala Cárdenas Suzana Maria Fleury Malheiros Reynaldo André Brandt Edson Amaro Junior Lionel Fernel Gamarra Tumor growth analysis by magnetic resonance imaging of the C6 glioblastoma model with prospects for the assessment of magnetohyperthermia therapy Einstein (São Paulo) Magnetic resonance imaging Glioblastoma Brain neoplasms Rats, Wistar |
| title | Tumor growth analysis by magnetic resonance imaging of the C6 glioblastoma model with prospects for the assessment of magnetohyperthermia therapy |
| title_full | Tumor growth analysis by magnetic resonance imaging of the C6 glioblastoma model with prospects for the assessment of magnetohyperthermia therapy |
| title_fullStr | Tumor growth analysis by magnetic resonance imaging of the C6 glioblastoma model with prospects for the assessment of magnetohyperthermia therapy |
| title_full_unstemmed | Tumor growth analysis by magnetic resonance imaging of the C6 glioblastoma model with prospects for the assessment of magnetohyperthermia therapy |
| title_short | Tumor growth analysis by magnetic resonance imaging of the C6 glioblastoma model with prospects for the assessment of magnetohyperthermia therapy |
| title_sort | tumor growth analysis by magnetic resonance imaging of the c6 glioblastoma model with prospects for the assessment of magnetohyperthermia therapy |
| topic | Magnetic resonance imaging Glioblastoma Brain neoplasms Rats, Wistar |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-45082012000100004&tlng=en |
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