Metabolomics with LC-QTOF-MS permits the prediction of disease stage in aortic abdominal aneurysm based on plasma metabolic fingerprint.
Abdominal aortic aneurysm (AAA) is a permanent and localized aortic dilation, defined as aortic diameter ≥3 cm. It is an asymptomatic but potentially fatal condition because progressive enlargement of the abdominal aorta is spontaneously evolving towards rupture.Biomarkers may help to explain pathol...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2012-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3286447?pdf=render |
| _version_ | 1828910272713064448 |
|---|---|
| author | Michal Ciborowski Joanna Teul Jose Luis Martin-Ventura Jesús Egido Coral Barbas |
| author_facet | Michal Ciborowski Joanna Teul Jose Luis Martin-Ventura Jesús Egido Coral Barbas |
| author_sort | Michal Ciborowski |
| collection | DOAJ |
| description | Abdominal aortic aneurysm (AAA) is a permanent and localized aortic dilation, defined as aortic diameter ≥3 cm. It is an asymptomatic but potentially fatal condition because progressive enlargement of the abdominal aorta is spontaneously evolving towards rupture.Biomarkers may help to explain pathological processes of AAA expansion, and allow us to find novel therapeutic strategies or to determine the efficiency of current therapies. Metabolomics seems to be a good approach to find biomarkers of AAA. In this study, plasma samples of patients with large AAA, small AAA, and controls were fingerprinted with LC-QTOF-MS. Statistical analysis was used to compare metabolic fingerprints and select metabolites that showed a significant change. Results presented here reveal that LC-QTOF-MS based fingerprinting of plasma from AAA patients is a very good technique to distinguish small AAA, large AAA, and controls. With the use of validated PLS-DA models it was possible to classify patients according to the disease stage and predict properly the stage of additional AAA patients. Identified metabolites indicate a role for sphingolipids, lysophospholipids, cholesterol metabolites, and acylcarnitines in the development and progression of AAA. Moreover, guanidinosuccinic acid, which mimics nitric oxide in terms of its vasodilatory action, was found as a strong marker of large AAA. |
| first_indexed | 2024-12-13T18:40:55Z |
| format | Article |
| id | doaj.art-c070a2d27e8b489195e939de9cf4eef0 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-13T18:40:55Z |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-c070a2d27e8b489195e939de9cf4eef02022-12-21T23:35:13ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-0172e3198210.1371/journal.pone.0031982Metabolomics with LC-QTOF-MS permits the prediction of disease stage in aortic abdominal aneurysm based on plasma metabolic fingerprint.Michal CiborowskiJoanna TeulJose Luis Martin-VenturaJesús EgidoCoral BarbasAbdominal aortic aneurysm (AAA) is a permanent and localized aortic dilation, defined as aortic diameter ≥3 cm. It is an asymptomatic but potentially fatal condition because progressive enlargement of the abdominal aorta is spontaneously evolving towards rupture.Biomarkers may help to explain pathological processes of AAA expansion, and allow us to find novel therapeutic strategies or to determine the efficiency of current therapies. Metabolomics seems to be a good approach to find biomarkers of AAA. In this study, plasma samples of patients with large AAA, small AAA, and controls were fingerprinted with LC-QTOF-MS. Statistical analysis was used to compare metabolic fingerprints and select metabolites that showed a significant change. Results presented here reveal that LC-QTOF-MS based fingerprinting of plasma from AAA patients is a very good technique to distinguish small AAA, large AAA, and controls. With the use of validated PLS-DA models it was possible to classify patients according to the disease stage and predict properly the stage of additional AAA patients. Identified metabolites indicate a role for sphingolipids, lysophospholipids, cholesterol metabolites, and acylcarnitines in the development and progression of AAA. Moreover, guanidinosuccinic acid, which mimics nitric oxide in terms of its vasodilatory action, was found as a strong marker of large AAA.http://europepmc.org/articles/PMC3286447?pdf=render |
| spellingShingle | Michal Ciborowski Joanna Teul Jose Luis Martin-Ventura Jesús Egido Coral Barbas Metabolomics with LC-QTOF-MS permits the prediction of disease stage in aortic abdominal aneurysm based on plasma metabolic fingerprint. PLoS ONE |
| title | Metabolomics with LC-QTOF-MS permits the prediction of disease stage in aortic abdominal aneurysm based on plasma metabolic fingerprint. |
| title_full | Metabolomics with LC-QTOF-MS permits the prediction of disease stage in aortic abdominal aneurysm based on plasma metabolic fingerprint. |
| title_fullStr | Metabolomics with LC-QTOF-MS permits the prediction of disease stage in aortic abdominal aneurysm based on plasma metabolic fingerprint. |
| title_full_unstemmed | Metabolomics with LC-QTOF-MS permits the prediction of disease stage in aortic abdominal aneurysm based on plasma metabolic fingerprint. |
| title_short | Metabolomics with LC-QTOF-MS permits the prediction of disease stage in aortic abdominal aneurysm based on plasma metabolic fingerprint. |
| title_sort | metabolomics with lc qtof ms permits the prediction of disease stage in aortic abdominal aneurysm based on plasma metabolic fingerprint |
| url | http://europepmc.org/articles/PMC3286447?pdf=render |
| work_keys_str_mv | AT michalciborowski metabolomicswithlcqtofmspermitsthepredictionofdiseasestageinaorticabdominalaneurysmbasedonplasmametabolicfingerprint AT joannateul metabolomicswithlcqtofmspermitsthepredictionofdiseasestageinaorticabdominalaneurysmbasedonplasmametabolicfingerprint AT joseluismartinventura metabolomicswithlcqtofmspermitsthepredictionofdiseasestageinaorticabdominalaneurysmbasedonplasmametabolicfingerprint AT jesusegido metabolomicswithlcqtofmspermitsthepredictionofdiseasestageinaorticabdominalaneurysmbasedonplasmametabolicfingerprint AT coralbarbas metabolomicswithlcqtofmspermitsthepredictionofdiseasestageinaorticabdominalaneurysmbasedonplasmametabolicfingerprint |