A Multi-Mineral Intervention to Modulate Colonic Mucosal Protein Profile: Results from a 90-Day Trial in Human Subjects
The overall goal of this study was to determine whether Aquamin<sup>®</sup>, a calcium-, magnesium-, trace element-rich, red algae-derived natural product, would alter the expression of proteins involved in growth-regulation and differentiation in colon. Thirty healthy human subjects (at...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-03-01
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| Series: | Nutrients |
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| Online Access: | https://www.mdpi.com/2072-6643/13/3/939 |
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| author | Muhammad N. Aslam Shannon D. McClintock Mohamed Ali H. Jawad-Makki Karsten Knuver Haris M. Ahmad Venkatesha Basrur Ingrid L. Bergin Suzanna M. Zick Ananda Sen D. Kim Turgeon James Varani |
| author_facet | Muhammad N. Aslam Shannon D. McClintock Mohamed Ali H. Jawad-Makki Karsten Knuver Haris M. Ahmad Venkatesha Basrur Ingrid L. Bergin Suzanna M. Zick Ananda Sen D. Kim Turgeon James Varani |
| author_sort | Muhammad N. Aslam |
| collection | DOAJ |
| description | The overall goal of this study was to determine whether Aquamin<sup>®</sup>, a calcium-, magnesium-, trace element-rich, red algae-derived natural product, would alter the expression of proteins involved in growth-regulation and differentiation in colon. Thirty healthy human subjects (at risk for colorectal cancer) were enrolled in a three-arm, 90-day interventional trial. Aquamin<sup>®</sup> was compared to calcium alone and placebo. Before and after the interventional period, colonic biopsies were obtained. Biopsies were evaluated by immunohistology for expression of Ki67 (proliferation marker) and for CK20 and p21 (differentiation markers). Tandem mass tag-mass spectrometry-based detection was used to assess levels of multiple proteins. As compared to placebo or calcium, Aquamin<sup>®</sup> reduced the level of Ki67 expression and slightly increased CK20 expression. Increased p21 expression was observed with both calcium and Aquamin<sup>®</sup>. In proteomic screen, Aquamin<sup>®</sup> treatment resulted in many more proteins being upregulated (including pro-apoptotic, cytokeratins, cell–cell adhesion molecules, and components of the basement membrane) or downregulated (proliferation and nucleic acid metabolism) than placebo. Calcium alone also altered the expression of many of the same proteins but not to the same extent as Aquamin<sup>®</sup>. We conclude that daily Aquamin<sup>®</sup> ingestion alters protein expression profile in the colon that could be beneficial to colonic health. |
| first_indexed | 2024-03-10T13:14:50Z |
| format | Article |
| id | doaj.art-c073c045175f47b1a9b492878b2edd47 |
| institution | Directory Open Access Journal |
| issn | 2072-6643 |
| language | English |
| last_indexed | 2024-03-10T13:14:50Z |
| publishDate | 2021-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nutrients |
| spelling | doaj.art-c073c045175f47b1a9b492878b2edd472023-11-21T10:29:02ZengMDPI AGNutrients2072-66432021-03-0113393910.3390/nu13030939A Multi-Mineral Intervention to Modulate Colonic Mucosal Protein Profile: Results from a 90-Day Trial in Human SubjectsMuhammad N. Aslam0Shannon D. McClintock1Mohamed Ali H. Jawad-Makki2Karsten Knuver3Haris M. Ahmad4Venkatesha Basrur5Ingrid L. Bergin6Suzanna M. Zick7Ananda Sen8D. Kim Turgeon9James Varani10Department of Pathology, The University of Michigan Medical School, Ann Arbor, MI 48109, USADepartment of Pathology, The University of Michigan Medical School, Ann Arbor, MI 48109, USADepartment of Pathology, The University of Michigan Medical School, Ann Arbor, MI 48109, USADepartment of Pathology, The University of Michigan Medical School, Ann Arbor, MI 48109, USADepartment of Pathology, The University of Michigan Medical School, Ann Arbor, MI 48109, USADepartment of Pathology, The University of Michigan Medical School, Ann Arbor, MI 48109, USAThe Unit for Laboratory Animal Medicine, The University of Michigan Medical School, Ann Arbor, MI 48109, USADepartment of Family Medicine, The University of Michigan Medical School, Ann Arbor, MI 48109, USADepartment of Family Medicine, The University of Michigan Medical School, Ann Arbor, MI 48109, USADepartment of Internal Medicine (Division of Gastroenterology), The University of Michigan Medical School, Ann Arbor, MI 48109, USADepartment of Pathology, The University of Michigan Medical School, Ann Arbor, MI 48109, USAThe overall goal of this study was to determine whether Aquamin<sup>®</sup>, a calcium-, magnesium-, trace element-rich, red algae-derived natural product, would alter the expression of proteins involved in growth-regulation and differentiation in colon. Thirty healthy human subjects (at risk for colorectal cancer) were enrolled in a three-arm, 90-day interventional trial. Aquamin<sup>®</sup> was compared to calcium alone and placebo. Before and after the interventional period, colonic biopsies were obtained. Biopsies were evaluated by immunohistology for expression of Ki67 (proliferation marker) and for CK20 and p21 (differentiation markers). Tandem mass tag-mass spectrometry-based detection was used to assess levels of multiple proteins. As compared to placebo or calcium, Aquamin<sup>®</sup> reduced the level of Ki67 expression and slightly increased CK20 expression. Increased p21 expression was observed with both calcium and Aquamin<sup>®</sup>. In proteomic screen, Aquamin<sup>®</sup> treatment resulted in many more proteins being upregulated (including pro-apoptotic, cytokeratins, cell–cell adhesion molecules, and components of the basement membrane) or downregulated (proliferation and nucleic acid metabolism) than placebo. Calcium alone also altered the expression of many of the same proteins but not to the same extent as Aquamin<sup>®</sup>. We conclude that daily Aquamin<sup>®</sup> ingestion alters protein expression profile in the colon that could be beneficial to colonic health.https://www.mdpi.com/2072-6643/13/3/939Aquamin<sup>®</sup>biomarkerscalciumcolon cancer chemopreventionmineralsproteomic analysis |
| spellingShingle | Muhammad N. Aslam Shannon D. McClintock Mohamed Ali H. Jawad-Makki Karsten Knuver Haris M. Ahmad Venkatesha Basrur Ingrid L. Bergin Suzanna M. Zick Ananda Sen D. Kim Turgeon James Varani A Multi-Mineral Intervention to Modulate Colonic Mucosal Protein Profile: Results from a 90-Day Trial in Human Subjects Nutrients Aquamin<sup>®</sup> biomarkers calcium colon cancer chemoprevention minerals proteomic analysis |
| title | A Multi-Mineral Intervention to Modulate Colonic Mucosal Protein Profile: Results from a 90-Day Trial in Human Subjects |
| title_full | A Multi-Mineral Intervention to Modulate Colonic Mucosal Protein Profile: Results from a 90-Day Trial in Human Subjects |
| title_fullStr | A Multi-Mineral Intervention to Modulate Colonic Mucosal Protein Profile: Results from a 90-Day Trial in Human Subjects |
| title_full_unstemmed | A Multi-Mineral Intervention to Modulate Colonic Mucosal Protein Profile: Results from a 90-Day Trial in Human Subjects |
| title_short | A Multi-Mineral Intervention to Modulate Colonic Mucosal Protein Profile: Results from a 90-Day Trial in Human Subjects |
| title_sort | multi mineral intervention to modulate colonic mucosal protein profile results from a 90 day trial in human subjects |
| topic | Aquamin<sup>®</sup> biomarkers calcium colon cancer chemoprevention minerals proteomic analysis |
| url | https://www.mdpi.com/2072-6643/13/3/939 |
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