Nanotoxicity of ZrS<sub>3</sub> Probed in a Bioluminescence Test on <i>E. coli</i> Bacteria: The Effect of Evolving H<sub>2</sub>S
Materials from a large family of transition metal trichalcogenides (TMTCs) attract considerable attention because of their potential applications in electronics, optoelectronics and energy storage, but information on their toxicity is lacking. In this study, we investigated the toxicity of ZrS<su...
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MDPI AG
2020-07-01
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| Series: | Nanomaterials |
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| Online Access: | https://www.mdpi.com/2079-4991/10/7/1401 |
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| author | Olga V. Zakharova Alexander A. Gusev Jehad Abourahma Nataliia S. Vorobeva Dmitry V. Sokolov Dmitry S. Muratov Denis V. Kuznetsov Alexander Sinitskii |
| author_facet | Olga V. Zakharova Alexander A. Gusev Jehad Abourahma Nataliia S. Vorobeva Dmitry V. Sokolov Dmitry S. Muratov Denis V. Kuznetsov Alexander Sinitskii |
| author_sort | Olga V. Zakharova |
| collection | DOAJ |
| description | Materials from a large family of transition metal trichalcogenides (TMTCs) attract considerable attention because of their potential applications in electronics, optoelectronics and energy storage, but information on their toxicity is lacking. In this study, we investigated the toxicity of ZrS<sub>3</sub>, a prominent TMTC material, toward photoluminescent <i>E. coli</i> bacteria in a bioluminescence test. We found that freshly prepared ZrS<sub>3</sub> suspensions in physiological saline solution with concentrations as high as 1 g/L did not exhibit any toxic effects on the bacteria. However, ZrS<sub>3</sub> suspensions that were stored for 24 h prior to the bioluminescence tests were very toxic to the bacteria and inhibited their emission, even at concentrations down to 0.001 g/L. We explain these observations by the aqueous hydrolysis of ZrS<sub>3</sub>, which resulted in the formation of ZrO<sub>x</sub> on the surface of ZrS<sub>3</sub> particles and the release of toxic H<sub>2</sub>S. The formation of ZrO<sub>x</sub> was confirmed by the XPS analysis, while the characteristic H<sub>2</sub>S smell was noticeable for the 24 h suspensions. This study demonstrates that while ZrS<sub>3</sub> appears to be intrinsically nontoxic to photoluminescent <i>E. coli</i> bacteria, it may exhibit high toxicity in aqueous media. The results of this study can likely be extended to other transition metal chalcogenides, as their toxicity in aqueous solutions may also increase over time due to hydrolysis and the formation of H<sub>2</sub>S. The results of this study also demonstrate that since many systems involving nanomaterials are unstable and evolve over time in various ways, their toxicity may evolve as well, which should be considered for relevant toxicity tests. |
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| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-03-10T18:24:02Z |
| publishDate | 2020-07-01 |
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| series | Nanomaterials |
| spelling | doaj.art-c0740fb633a244c48c0b9b62e80cb9e92023-11-20T07:12:53ZengMDPI AGNanomaterials2079-49912020-07-01107140110.3390/nano10071401Nanotoxicity of ZrS<sub>3</sub> Probed in a Bioluminescence Test on <i>E. coli</i> Bacteria: The Effect of Evolving H<sub>2</sub>SOlga V. Zakharova0Alexander A. Gusev1Jehad Abourahma2Nataliia S. Vorobeva3Dmitry V. Sokolov4Dmitry S. Muratov5Denis V. Kuznetsov6Alexander Sinitskii7Research Institute for Environmental Science and Biotechnology, Derzhavin Tambov State University, Tambov 392000, RussiaResearch Institute for Environmental Science and Biotechnology, Derzhavin Tambov State University, Tambov 392000, RussiaDepartment of Chemistry, University of Nebraska—Lincoln, Lincoln, NE 68588, USADepartment of Chemistry, University of Nebraska—Lincoln, Lincoln, NE 68588, USADepartment of Functional Nanosystems and High-Temperature Materials, National University of Science and Technology “MISIS”, Moscow 119991, RussiaDepartment of Functional Nanosystems and High-Temperature Materials, National University of Science and Technology “MISIS”, Moscow 119991, RussiaDepartment of Functional Nanosystems and High-Temperature Materials, National University of Science and Technology “MISIS”, Moscow 119991, RussiaDepartment of Chemistry, University of Nebraska—Lincoln, Lincoln, NE 68588, USAMaterials from a large family of transition metal trichalcogenides (TMTCs) attract considerable attention because of their potential applications in electronics, optoelectronics and energy storage, but information on their toxicity is lacking. In this study, we investigated the toxicity of ZrS<sub>3</sub>, a prominent TMTC material, toward photoluminescent <i>E. coli</i> bacteria in a bioluminescence test. We found that freshly prepared ZrS<sub>3</sub> suspensions in physiological saline solution with concentrations as high as 1 g/L did not exhibit any toxic effects on the bacteria. However, ZrS<sub>3</sub> suspensions that were stored for 24 h prior to the bioluminescence tests were very toxic to the bacteria and inhibited their emission, even at concentrations down to 0.001 g/L. We explain these observations by the aqueous hydrolysis of ZrS<sub>3</sub>, which resulted in the formation of ZrO<sub>x</sub> on the surface of ZrS<sub>3</sub> particles and the release of toxic H<sub>2</sub>S. The formation of ZrO<sub>x</sub> was confirmed by the XPS analysis, while the characteristic H<sub>2</sub>S smell was noticeable for the 24 h suspensions. This study demonstrates that while ZrS<sub>3</sub> appears to be intrinsically nontoxic to photoluminescent <i>E. coli</i> bacteria, it may exhibit high toxicity in aqueous media. The results of this study can likely be extended to other transition metal chalcogenides, as their toxicity in aqueous solutions may also increase over time due to hydrolysis and the formation of H<sub>2</sub>S. The results of this study also demonstrate that since many systems involving nanomaterials are unstable and evolve over time in various ways, their toxicity may evolve as well, which should be considered for relevant toxicity tests.https://www.mdpi.com/2079-4991/10/7/1401zirconium trisulfidenanotoxicityantibacterial propertiesbioluminescence test<i>Escherichia coli</i> |
| spellingShingle | Olga V. Zakharova Alexander A. Gusev Jehad Abourahma Nataliia S. Vorobeva Dmitry V. Sokolov Dmitry S. Muratov Denis V. Kuznetsov Alexander Sinitskii Nanotoxicity of ZrS<sub>3</sub> Probed in a Bioluminescence Test on <i>E. coli</i> Bacteria: The Effect of Evolving H<sub>2</sub>S Nanomaterials zirconium trisulfide nanotoxicity antibacterial properties bioluminescence test <i>Escherichia coli</i> |
| title | Nanotoxicity of ZrS<sub>3</sub> Probed in a Bioluminescence Test on <i>E. coli</i> Bacteria: The Effect of Evolving H<sub>2</sub>S |
| title_full | Nanotoxicity of ZrS<sub>3</sub> Probed in a Bioluminescence Test on <i>E. coli</i> Bacteria: The Effect of Evolving H<sub>2</sub>S |
| title_fullStr | Nanotoxicity of ZrS<sub>3</sub> Probed in a Bioluminescence Test on <i>E. coli</i> Bacteria: The Effect of Evolving H<sub>2</sub>S |
| title_full_unstemmed | Nanotoxicity of ZrS<sub>3</sub> Probed in a Bioluminescence Test on <i>E. coli</i> Bacteria: The Effect of Evolving H<sub>2</sub>S |
| title_short | Nanotoxicity of ZrS<sub>3</sub> Probed in a Bioluminescence Test on <i>E. coli</i> Bacteria: The Effect of Evolving H<sub>2</sub>S |
| title_sort | nanotoxicity of zrs sub 3 sub probed in a bioluminescence test on i e coli i bacteria the effect of evolving h sub 2 sub s |
| topic | zirconium trisulfide nanotoxicity antibacterial properties bioluminescence test <i>Escherichia coli</i> |
| url | https://www.mdpi.com/2079-4991/10/7/1401 |
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