Flow Injection Spectrophotometric Determination of N-Acetyl-L-cysteine as a Complex with Palladium(II)
We describe a new method using flow-injection analysis with spectro-photometric detection, suitable for the determination of N-acetyl-L-cysteine (NAC). The proposed method is appropriate for the determination of NAC in reaction with Pd2+ ions in the concentration range from 1.0 × 10−5 mol L−1 to 6.0...
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| Format: | Article |
| Language: | English |
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MDPI AG
2011-08-01
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| Series: | Molecules |
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| Online Access: | http://www.mdpi.com/1420-3049/16/9/7224/ |
| _version_ | 1811196725778448384 |
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| author | Ante Prkić Mia Brkljača Josipa Giljanović |
| author_facet | Ante Prkić Mia Brkljača Josipa Giljanović |
| author_sort | Ante Prkić |
| collection | DOAJ |
| description | We describe a new method using flow-injection analysis with spectro-photometric detection, suitable for the determination of N-acetyl-L-cysteine (NAC). The proposed method is appropriate for the determination of NAC in reaction with Pd2+ ions in the concentration range from 1.0 × 10−5 mol L−1 to 6.0 × 10−5 mol L−1. The detection limit NAC was 5.84 × 10−6 mol L−1 and the recorded relative standard deviation of the method is in the range from 1.67 to 4.11%. NAC and Pd2+ form complexes of Pd2+:NAC molar ratios of 1:1 and 1:2, depending on the ratio of their analytical concentrations. The cumulative conditional stability constant for the Pd(NAC)22+ complex is β12' = 2.69 × 109 L2 mol−2. The proposed method was compared with the classic spectrophotometric determination of NAC, using the same reagent, PdCl2, and had shown certain advantages: a) shorter analysis time; b) the use of smaller volumes of sample and reagents, which make the proposed method cheaper and faster for NAC determination in real samples without sample pretreatment. |
| first_indexed | 2024-04-12T01:03:49Z |
| format | Article |
| id | doaj.art-c074a3c8312b4214a9013f73163706da |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-04-12T01:03:49Z |
| publishDate | 2011-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-c074a3c8312b4214a9013f73163706da2022-12-22T03:54:22ZengMDPI AGMolecules1420-30492011-08-011697224723610.3390/molecules16097224Flow Injection Spectrophotometric Determination of N-Acetyl-L-cysteine as a Complex with Palladium(II)Ante PrkićMia BrkljačaJosipa GiljanovićWe describe a new method using flow-injection analysis with spectro-photometric detection, suitable for the determination of N-acetyl-L-cysteine (NAC). The proposed method is appropriate for the determination of NAC in reaction with Pd2+ ions in the concentration range from 1.0 × 10−5 mol L−1 to 6.0 × 10−5 mol L−1. The detection limit NAC was 5.84 × 10−6 mol L−1 and the recorded relative standard deviation of the method is in the range from 1.67 to 4.11%. NAC and Pd2+ form complexes of Pd2+:NAC molar ratios of 1:1 and 1:2, depending on the ratio of their analytical concentrations. The cumulative conditional stability constant for the Pd(NAC)22+ complex is β12' = 2.69 × 109 L2 mol−2. The proposed method was compared with the classic spectrophotometric determination of NAC, using the same reagent, PdCl2, and had shown certain advantages: a) shorter analysis time; b) the use of smaller volumes of sample and reagents, which make the proposed method cheaper and faster for NAC determination in real samples without sample pretreatment.http://www.mdpi.com/1420-3049/16/9/7224/flow-injectionspectrophotometryN-Acetyl-L-cysteinepalladiumcomplexometry |
| spellingShingle | Ante Prkić Mia Brkljača Josipa Giljanović Flow Injection Spectrophotometric Determination of N-Acetyl-L-cysteine as a Complex with Palladium(II) Molecules flow-injection spectrophotometry N-Acetyl-L-cysteine palladium complexometry |
| title | Flow Injection Spectrophotometric Determination of N-Acetyl-L-cysteine as a Complex with Palladium(II) |
| title_full | Flow Injection Spectrophotometric Determination of N-Acetyl-L-cysteine as a Complex with Palladium(II) |
| title_fullStr | Flow Injection Spectrophotometric Determination of N-Acetyl-L-cysteine as a Complex with Palladium(II) |
| title_full_unstemmed | Flow Injection Spectrophotometric Determination of N-Acetyl-L-cysteine as a Complex with Palladium(II) |
| title_short | Flow Injection Spectrophotometric Determination of N-Acetyl-L-cysteine as a Complex with Palladium(II) |
| title_sort | flow injection spectrophotometric determination of n acetyl l cysteine as a complex with palladium ii |
| topic | flow-injection spectrophotometry N-Acetyl-L-cysteine palladium complexometry |
| url | http://www.mdpi.com/1420-3049/16/9/7224/ |
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