Production of proline and protease with different organic wastes in bacteria (Production proline and protease with organic wastes)
Abstract In this study, we investigated the proline and protease production of different bacteria in several organic waste materials. Our aim was to produce proline and protease economically in waste that is abundantly available while reducing its environmental impact. 5 ml of different organic wast...
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| Format: | Article |
| Language: | English |
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Instituto Internacional de Ecologia
2021-06-01
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| Series: | Brazilian Journal of Biology |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-69842022000100216&tlng=en |
| _version_ | 1818973708409110528 |
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| author | H. Kahraman C. C. Karaderi |
| author_facet | H. Kahraman C. C. Karaderi |
| author_sort | H. Kahraman |
| collection | DOAJ |
| description | Abstract In this study, we investigated the proline and protease production of different bacteria in several organic waste materials. Our aim was to produce proline and protease economically in waste that is abundantly available while reducing its environmental impact. 5 ml of different organic waste materials (OWW: Olive waste water; N.B: Nutrient Broth; EW: Eggshell; PBS: PBS buffer; PLW: Peach leaf wastes; TCW: Turkish coffee wastes; TWW: Tea waste water; WCW: Waste cheese whey; WFO: Waste frying oil) were placed in 10 ml grow tubes, inoculated and incubated for 24 h. Phosphate-buffered saline and 10% solutions of different organic wastes were added. These cultures were subsequently incubated at 37°C for 24 h. Cells were harvested at 24 h for L-proline assay. 1 ml of culture was transferred by pipette into an Eppendorf tube and centrifuged at 14,000 rpm for 20 min at room temperature. Cellular debris was removed by centrifuge and the supernatant was used for proline activity assays. Protease activity was determined using a modified method with casein as the substrate. We found that proline and protease can easily be produced economically using Turkish coffee wastes (TCW), Waste cheese whey (WCW) and Olive waste water (OWW) organic waste. We believe that this study will result in similar research leading to the economical use of these waste materials thus reducing their impact on the environment. |
| first_indexed | 2024-12-20T15:28:27Z |
| format | Article |
| id | doaj.art-c075414c6fcc40f1bff80de7ff852f6e |
| institution | Directory Open Access Journal |
| issn | 1678-4375 |
| language | English |
| last_indexed | 2024-12-20T15:28:27Z |
| publishDate | 2021-06-01 |
| publisher | Instituto Internacional de Ecologia |
| record_format | Article |
| series | Brazilian Journal of Biology |
| spelling | doaj.art-c075414c6fcc40f1bff80de7ff852f6e2022-12-21T19:35:44ZengInstituto Internacional de EcologiaBrazilian Journal of Biology1678-43752021-06-018210.1590/1519-6984.243187Production of proline and protease with different organic wastes in bacteria (Production proline and protease with organic wastes)H. Kahramanhttps://orcid.org/0000-0001-6235-5497C. C. Karaderihttps://orcid.org/0000-0002-4841-4435Abstract In this study, we investigated the proline and protease production of different bacteria in several organic waste materials. Our aim was to produce proline and protease economically in waste that is abundantly available while reducing its environmental impact. 5 ml of different organic waste materials (OWW: Olive waste water; N.B: Nutrient Broth; EW: Eggshell; PBS: PBS buffer; PLW: Peach leaf wastes; TCW: Turkish coffee wastes; TWW: Tea waste water; WCW: Waste cheese whey; WFO: Waste frying oil) were placed in 10 ml grow tubes, inoculated and incubated for 24 h. Phosphate-buffered saline and 10% solutions of different organic wastes were added. These cultures were subsequently incubated at 37°C for 24 h. Cells were harvested at 24 h for L-proline assay. 1 ml of culture was transferred by pipette into an Eppendorf tube and centrifuged at 14,000 rpm for 20 min at room temperature. Cellular debris was removed by centrifuge and the supernatant was used for proline activity assays. Protease activity was determined using a modified method with casein as the substrate. We found that proline and protease can easily be produced economically using Turkish coffee wastes (TCW), Waste cheese whey (WCW) and Olive waste water (OWW) organic waste. We believe that this study will result in similar research leading to the economical use of these waste materials thus reducing their impact on the environment.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-69842022000100216&tlng=enorganic wastesbacteriaprolineprotease |
| spellingShingle | H. Kahraman C. C. Karaderi Production of proline and protease with different organic wastes in bacteria (Production proline and protease with organic wastes) Brazilian Journal of Biology organic wastes bacteria proline protease |
| title | Production of proline and protease with different organic wastes in bacteria (Production proline and protease with organic wastes) |
| title_full | Production of proline and protease with different organic wastes in bacteria (Production proline and protease with organic wastes) |
| title_fullStr | Production of proline and protease with different organic wastes in bacteria (Production proline and protease with organic wastes) |
| title_full_unstemmed | Production of proline and protease with different organic wastes in bacteria (Production proline and protease with organic wastes) |
| title_short | Production of proline and protease with different organic wastes in bacteria (Production proline and protease with organic wastes) |
| title_sort | production of proline and protease with different organic wastes in bacteria production proline and protease with organic wastes |
| topic | organic wastes bacteria proline protease |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-69842022000100216&tlng=en |
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