Seed Transmission of Beet Curly Top Virus and Beet Curly Top Iran Virus in a Local Cultivar of Petunia in Iran
Beet curly top virus (BCTV) and beet curly top Iran virus (BCTIV) are known as the causal agents of curly top disease in beet and several other dicotyledonous plants in Iran. These viruses are transmitted by Circulifer species, and until now, there has been no confirmed report of their seed transmis...
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| Format: | Article |
| Language: | English |
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MDPI AG
2017-10-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/9/10/299 |
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| author | Ameneh Anabestani Seyed Ali Akbar Behjatnia Keramat Izadpanah Saeid Tabein Gian Paolo Accotto |
| author_facet | Ameneh Anabestani Seyed Ali Akbar Behjatnia Keramat Izadpanah Saeid Tabein Gian Paolo Accotto |
| author_sort | Ameneh Anabestani |
| collection | DOAJ |
| description | Beet curly top virus (BCTV) and beet curly top Iran virus (BCTIV) are known as the causal agents of curly top disease in beet and several other dicotyledonous plants in Iran. These viruses are transmitted by Circulifer species, and until now, there has been no confirmed report of their seed transmission. A percentage (38.2–78.0%) of the seedlings developed from the seeds of a petunia local cultivar under insect-free conditions showed stunting, interveinal chlorosis, leaf curling, and vein swelling symptoms, and were infected by BCTV when tested by PCR. Presence of BCTV in seed extracts of petunia local cultivar was confirmed by PCR and IC-PCR, followed by sequencing. Agroinoculation of curly top free petunia plants with a BCTV infectious clone resulted in BCTV infection of plants and their developed seeds. These results show the seed infection and transmission of BCTV in a local cultivar of petunia. Similar experiments performed with BCTIV showed that this virus is also seed transmissible in the same cultivar of petunia, although with a lower rate (8.8–18.5%). Seed transmission of curly top viruses may have significant implications in the epidemiology of these viruses. |
| first_indexed | 2024-12-23T06:09:32Z |
| format | Article |
| id | doaj.art-c0785e37291643dca8233ad2504caec6 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-12-23T06:09:32Z |
| publishDate | 2017-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-c0785e37291643dca8233ad2504caec62022-12-21T17:57:30ZengMDPI AGViruses1999-49152017-10-0191029910.3390/v9100299v9100299Seed Transmission of Beet Curly Top Virus and Beet Curly Top Iran Virus in a Local Cultivar of Petunia in IranAmeneh Anabestani0Seyed Ali Akbar Behjatnia1Keramat Izadpanah2Saeid Tabein3Gian Paolo Accotto4Plant Virology Research Center, College of Agriculture, Shiraz University, Shiraz 71441-65186, IranPlant Virology Research Center, College of Agriculture, Shiraz University, Shiraz 71441-65186, IranPlant Virology Research Center, College of Agriculture, Shiraz University, Shiraz 71441-65186, IranPlant Virology Research Center, College of Agriculture, Shiraz University, Shiraz 71441-65186, IranInstitute for Sustainable Plant Protection, National Research Council of Italy (IPSP-CNR), 10135 Torino, ItalyBeet curly top virus (BCTV) and beet curly top Iran virus (BCTIV) are known as the causal agents of curly top disease in beet and several other dicotyledonous plants in Iran. These viruses are transmitted by Circulifer species, and until now, there has been no confirmed report of their seed transmission. A percentage (38.2–78.0%) of the seedlings developed from the seeds of a petunia local cultivar under insect-free conditions showed stunting, interveinal chlorosis, leaf curling, and vein swelling symptoms, and were infected by BCTV when tested by PCR. Presence of BCTV in seed extracts of petunia local cultivar was confirmed by PCR and IC-PCR, followed by sequencing. Agroinoculation of curly top free petunia plants with a BCTV infectious clone resulted in BCTV infection of plants and their developed seeds. These results show the seed infection and transmission of BCTV in a local cultivar of petunia. Similar experiments performed with BCTIV showed that this virus is also seed transmissible in the same cultivar of petunia, although with a lower rate (8.8–18.5%). Seed transmission of curly top viruses may have significant implications in the epidemiology of these viruses.https://www.mdpi.com/1999-4915/9/10/299geminivirusespetuniabeet curly top virusesseed transmissiontransmission rate |
| spellingShingle | Ameneh Anabestani Seyed Ali Akbar Behjatnia Keramat Izadpanah Saeid Tabein Gian Paolo Accotto Seed Transmission of Beet Curly Top Virus and Beet Curly Top Iran Virus in a Local Cultivar of Petunia in Iran Viruses geminiviruses petunia beet curly top viruses seed transmission transmission rate |
| title | Seed Transmission of Beet Curly Top Virus and Beet Curly Top Iran Virus in a Local Cultivar of Petunia in Iran |
| title_full | Seed Transmission of Beet Curly Top Virus and Beet Curly Top Iran Virus in a Local Cultivar of Petunia in Iran |
| title_fullStr | Seed Transmission of Beet Curly Top Virus and Beet Curly Top Iran Virus in a Local Cultivar of Petunia in Iran |
| title_full_unstemmed | Seed Transmission of Beet Curly Top Virus and Beet Curly Top Iran Virus in a Local Cultivar of Petunia in Iran |
| title_short | Seed Transmission of Beet Curly Top Virus and Beet Curly Top Iran Virus in a Local Cultivar of Petunia in Iran |
| title_sort | seed transmission of beet curly top virus and beet curly top iran virus in a local cultivar of petunia in iran |
| topic | geminiviruses petunia beet curly top viruses seed transmission transmission rate |
| url | https://www.mdpi.com/1999-4915/9/10/299 |
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