Factors That Shape Eukaryotic tRNAomes: Processing, Modification and Anticodon–Codon Use
Transfer RNAs (tRNAs) contain sequence diversity beyond their anticodons and the large variety of nucleotide modifications found in all kingdoms of life. Some modifications stabilize structure and fit in the ribosome whereas those to the anticodon loop modulate messenger RNA (mRNA) decoding activity...
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| Format: | Article |
| Language: | English |
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MDPI AG
2017-03-01
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| Series: | Biomolecules |
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| Online Access: | http://www.mdpi.com/2218-273X/7/1/26 |
| _version_ | 1818128787912523776 |
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| author | Richard J. Maraia Aneeshkumar G. Arimbasseri |
| author_facet | Richard J. Maraia Aneeshkumar G. Arimbasseri |
| author_sort | Richard J. Maraia |
| collection | DOAJ |
| description | Transfer RNAs (tRNAs) contain sequence diversity beyond their anticodons and the large variety of nucleotide modifications found in all kingdoms of life. Some modifications stabilize structure and fit in the ribosome whereas those to the anticodon loop modulate messenger RNA (mRNA) decoding activity more directly. The identities of tRNAs with some universal anticodon loop modifications vary among distant and parallel species, likely to accommodate fine tuning for their translation systems. This plasticity in positions 34 (wobble) and 37 is reflected in codon use bias. Here, we review convergent evidence that suggest that expansion of the eukaryotic tRNAome was supported by its dedicated RNA polymerase III transcription system and coupling to the precursor‐tRNA chaperone, La protein. We also review aspects of eukaryotic tRNAome evolution involving G34/A34 anticodon‐sparing, relation to A34 modification to inosine, biased codon use and regulatory information in the redundancy (synonymous) component of the genetic code. We then review interdependent anticodon loop modifications involving position 37 in eukaryotes. This includes the eukaryote‐specific tRNA modification, 3‐methylcytidine‐32 (m3C32) and the responsible gene, TRM140 and homologs which were duplicated and subspecialized for isoacceptor‐specific substrates and dependence on i6A37 or t6A37. The genetics of tRNA function is relevant to health directly and as disease modifiers. |
| first_indexed | 2024-12-11T07:38:48Z |
| format | Article |
| id | doaj.art-c079478a259848c58f1f89dcea7db47e |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-12-11T07:38:48Z |
| publishDate | 2017-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-c079478a259848c58f1f89dcea7db47e2022-12-22T01:15:39ZengMDPI AGBiomolecules2218-273X2017-03-01712610.3390/biom7010026biom7010026Factors That Shape Eukaryotic tRNAomes: Processing, Modification and Anticodon–Codon UseRichard J. Maraia0Aneeshkumar G. Arimbasseri1Intramural Research Program, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD, 20892, USAMolecular Genetics Laboratory, National Institute of Immunology, Aruna Asaf Ali Marg, New Delhi 110067, IndiaTransfer RNAs (tRNAs) contain sequence diversity beyond their anticodons and the large variety of nucleotide modifications found in all kingdoms of life. Some modifications stabilize structure and fit in the ribosome whereas those to the anticodon loop modulate messenger RNA (mRNA) decoding activity more directly. The identities of tRNAs with some universal anticodon loop modifications vary among distant and parallel species, likely to accommodate fine tuning for their translation systems. This plasticity in positions 34 (wobble) and 37 is reflected in codon use bias. Here, we review convergent evidence that suggest that expansion of the eukaryotic tRNAome was supported by its dedicated RNA polymerase III transcription system and coupling to the precursor‐tRNA chaperone, La protein. We also review aspects of eukaryotic tRNAome evolution involving G34/A34 anticodon‐sparing, relation to A34 modification to inosine, biased codon use and regulatory information in the redundancy (synonymous) component of the genetic code. We then review interdependent anticodon loop modifications involving position 37 in eukaryotes. This includes the eukaryote‐specific tRNA modification, 3‐methylcytidine‐32 (m3C32) and the responsible gene, TRM140 and homologs which were duplicated and subspecialized for isoacceptor‐specific substrates and dependence on i6A37 or t6A37. The genetics of tRNA function is relevant to health directly and as disease modifiers.http://www.mdpi.com/2218-273X/7/1/26anticodon sparing adenosine 34 inosine 34 tRNA adenosine deaminase tRNA methyltransferase La protein RNA chaperone |
| spellingShingle | Richard J. Maraia Aneeshkumar G. Arimbasseri Factors That Shape Eukaryotic tRNAomes: Processing, Modification and Anticodon–Codon Use Biomolecules anticodon sparing adenosine 34 inosine 34 tRNA adenosine deaminase tRNA methyltransferase La protein RNA chaperone |
| title | Factors That Shape Eukaryotic tRNAomes: Processing, Modification and Anticodon–Codon Use |
| title_full | Factors That Shape Eukaryotic tRNAomes: Processing, Modification and Anticodon–Codon Use |
| title_fullStr | Factors That Shape Eukaryotic tRNAomes: Processing, Modification and Anticodon–Codon Use |
| title_full_unstemmed | Factors That Shape Eukaryotic tRNAomes: Processing, Modification and Anticodon–Codon Use |
| title_short | Factors That Shape Eukaryotic tRNAomes: Processing, Modification and Anticodon–Codon Use |
| title_sort | factors that shape eukaryotic trnaomes processing modification and anticodon codon use |
| topic | anticodon sparing adenosine 34 inosine 34 tRNA adenosine deaminase tRNA methyltransferase La protein RNA chaperone |
| url | http://www.mdpi.com/2218-273X/7/1/26 |
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