DNA Binding and Anticancer Properties of New Pd(II)-Phosphorus Schiff Base Metal Complexes
DNA has become the target of metal complexes in cancer drug discovery. Due to the side effects of widely known cisplatin and its derivative compounds, alternative metal-based drug discovery studies are still ongoing. In this study, the DNA-binding ability of Pd(II) and Pt(II) complexes of four phosp...
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MDPI AG
2022-11-01
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| Series: | Pharmaceutics |
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| author | Burcu Saygıdeğer Demir Simay İnce Mustafa Kemal Yilmaz Aycan Sezan Ezgi Derinöz Tugba Taskin-Tok Yasemin Saygideger |
| author_facet | Burcu Saygıdeğer Demir Simay İnce Mustafa Kemal Yilmaz Aycan Sezan Ezgi Derinöz Tugba Taskin-Tok Yasemin Saygideger |
| author_sort | Burcu Saygıdeğer Demir |
| collection | DOAJ |
| description | DNA has become the target of metal complexes in cancer drug discovery. Due to the side effects of widely known cisplatin and its derivative compounds, alternative metal-based drug discovery studies are still ongoing. In this study, the DNA-binding ability of Pd(II) and Pt(II) complexes of four phosphorus Schiff base ligands and four hydrazonoic-phosphines are investigated by using in silico analyses. Phosphorus Schiff base-Pd(II) complexes encoded as <b>B1</b> and <b>B2</b> with the best DNA-binding potential are synthesized and characterized. The DNA-binding potentials of these two new Pd(II) complexes are also investigated experimentally, and their antitumor properties are demonstrated in vitro in A549, MCF7, HuH7, and HCT116 cancer cells. The mechanisms of these metal complexes that kill the cells mentioned above in different activities are elucidated by flow cytometry apoptosis analysis and colony formation analysis The in silico binding energies of these two new palladium complexes ΔG (<b>B1</b>): −4.51 and ΔG (<b>B2</b>): −6.04 kcal/mol, and their experimental DNA-binding constants were found as Kb (<b>B1</b>): 4.24 × 10<sup>5</sup>, Kb (<b>B2</b>): 4.98 × 10<sup>5</sup>). The new complexes, which show different antitumor effects in different cells, are the least effective in HuH7 liver cells, while they showed the best antitumor properties in HCT116 colon cancer cells. |
| first_indexed | 2024-03-09T18:45:23Z |
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| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-03-09T18:45:23Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj.art-c07aaff794e34509ad22ea2de7a9e2c82023-11-24T06:21:48ZengMDPI AGPharmaceutics1999-49232022-11-011411240910.3390/pharmaceutics14112409DNA Binding and Anticancer Properties of New Pd(II)-Phosphorus Schiff Base Metal ComplexesBurcu Saygıdeğer Demir0Simay İnce1Mustafa Kemal Yilmaz2Aycan Sezan3Ezgi Derinöz4Tugba Taskin-Tok5Yasemin Saygideger6Department of Biotechnology, Institute of Natural and Applied Sciences, Çukurova University, Adana 01330, TurkeyDepartment of Nanotechnology and Advanced Materials, Institute of Science and Technology, Mersin University, Mersin 33343, TurkeyDepartment of Nanotechnology and Advanced Materials, Institute of Science and Technology, Mersin University, Mersin 33343, TurkeyDepartment of Biotechnology, Institute of Natural and Applied Sciences, Çukurova University, Adana 01330, TurkeyDepartment of Biotechnology, Institute of Natural and Applied Sciences, Çukurova University, Adana 01330, TurkeyDepartment of Chemistry, Faculty of Arts and Science, Gaziantep University, Gaziantep 27410, TurkeyDepartment of Biotechnology, Institute of Natural and Applied Sciences, Çukurova University, Adana 01330, TurkeyDNA has become the target of metal complexes in cancer drug discovery. Due to the side effects of widely known cisplatin and its derivative compounds, alternative metal-based drug discovery studies are still ongoing. In this study, the DNA-binding ability of Pd(II) and Pt(II) complexes of four phosphorus Schiff base ligands and four hydrazonoic-phosphines are investigated by using in silico analyses. Phosphorus Schiff base-Pd(II) complexes encoded as <b>B1</b> and <b>B2</b> with the best DNA-binding potential are synthesized and characterized. The DNA-binding potentials of these two new Pd(II) complexes are also investigated experimentally, and their antitumor properties are demonstrated in vitro in A549, MCF7, HuH7, and HCT116 cancer cells. The mechanisms of these metal complexes that kill the cells mentioned above in different activities are elucidated by flow cytometry apoptosis analysis and colony formation analysis The in silico binding energies of these two new palladium complexes ΔG (<b>B1</b>): −4.51 and ΔG (<b>B2</b>): −6.04 kcal/mol, and their experimental DNA-binding constants were found as Kb (<b>B1</b>): 4.24 × 10<sup>5</sup>, Kb (<b>B2</b>): 4.98 × 10<sup>5</sup>). The new complexes, which show different antitumor effects in different cells, are the least effective in HuH7 liver cells, while they showed the best antitumor properties in HCT116 colon cancer cells.https://www.mdpi.com/1999-4923/14/11/2409molecular dockingphosphorus Schiff basemetal complexescancer cellsantitumorDNA binding |
| spellingShingle | Burcu Saygıdeğer Demir Simay İnce Mustafa Kemal Yilmaz Aycan Sezan Ezgi Derinöz Tugba Taskin-Tok Yasemin Saygideger DNA Binding and Anticancer Properties of New Pd(II)-Phosphorus Schiff Base Metal Complexes Pharmaceutics molecular docking phosphorus Schiff base metal complexes cancer cells antitumor DNA binding |
| title | DNA Binding and Anticancer Properties of New Pd(II)-Phosphorus Schiff Base Metal Complexes |
| title_full | DNA Binding and Anticancer Properties of New Pd(II)-Phosphorus Schiff Base Metal Complexes |
| title_fullStr | DNA Binding and Anticancer Properties of New Pd(II)-Phosphorus Schiff Base Metal Complexes |
| title_full_unstemmed | DNA Binding and Anticancer Properties of New Pd(II)-Phosphorus Schiff Base Metal Complexes |
| title_short | DNA Binding and Anticancer Properties of New Pd(II)-Phosphorus Schiff Base Metal Complexes |
| title_sort | dna binding and anticancer properties of new pd ii phosphorus schiff base metal complexes |
| topic | molecular docking phosphorus Schiff base metal complexes cancer cells antitumor DNA binding |
| url | https://www.mdpi.com/1999-4923/14/11/2409 |
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