An Integrated biomarker approach for explaining the potency of exogenous glucose on transportation induced stress in Labeo rohita fingerlings
Abstract Transportation of fish seed is a complex phenomenon associated with multiple kinds of stressors that simultaneously affect the fish in a confined environment, causing stress and mortality. The present study investigated the stress-relieving effect of exogenous glucose as a water additive in...
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| Format: | Article |
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Nature Portfolio
2021-03-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-021-85311-5 |
| _version_ | 1818722371250421760 |
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| author | Abhilipsa Biswal Prem Prakash Srivastava Gopal Krishna Tapas Paul Prasenjit Pal Subodh Gupta Tincy Varghese Manish Jayant |
| author_facet | Abhilipsa Biswal Prem Prakash Srivastava Gopal Krishna Tapas Paul Prasenjit Pal Subodh Gupta Tincy Varghese Manish Jayant |
| author_sort | Abhilipsa Biswal |
| collection | DOAJ |
| description | Abstract Transportation of fish seed is a complex phenomenon associated with multiple kinds of stressors that simultaneously affect the fish in a confined environment, causing stress and mortality. The present study investigated the stress-relieving effect of exogenous glucose as a water additive in different concentrations (0.1, 0.2, 0.3, and 0.4%) during simulated transportation (12 h) of L. rohita fingerlings. The integrated biomarker response (IBR) index is a holistic tool to determine the optimum dose of exogenous glucose for mitigating transportation stress in fish. Based on selected biomarkers related to the stress hormone, serum biochemistry, oxidative stress, and HSP70 mRNA expression, the IBR index is calculated for each treatment and control group. The result showed a significant change in the level of stress hormone cortisol, enzymes (SGPT, LDH, MDH, SOD, CAT) and metabolites (serum glucose, triglyceride, creatinine) along with an upregulation in liver HSP70 mRNA expression. IBR index suggests that 0.2% glucose exhibited the lowest multi-biomarker stress response in comparison to other treatments and control. Therefore, the use of 0.2% glucose as a water additive will provide a solution to transportation induced stress in L. rohita fingerling and will underwrite the success of grow-out fish culture in days to come. |
| first_indexed | 2024-12-17T20:53:34Z |
| format | Article |
| id | doaj.art-c07b97ebbb364eb28bcb098b56e524b4 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-17T20:53:34Z |
| publishDate | 2021-03-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-c07b97ebbb364eb28bcb098b56e524b42022-12-21T21:32:57ZengNature PortfolioScientific Reports2045-23222021-03-0111111110.1038/s41598-021-85311-5An Integrated biomarker approach for explaining the potency of exogenous glucose on transportation induced stress in Labeo rohita fingerlingsAbhilipsa Biswal0Prem Prakash Srivastava1Gopal Krishna2Tapas Paul3Prasenjit Pal4Subodh Gupta5Tincy Varghese6Manish Jayant7ICAR-Central Institute of Fisheries EducationICAR-Central Institute of Fisheries EducationICAR-Central Institute of Fisheries EducationICAR-Central Institute of Fisheries EducationCollege of Fisheries, Central Agricultural UniversityICAR-Central Institute of Fisheries EducationICAR-Central Institute of Fisheries EducationICAR-Central Institute of Fisheries EducationAbstract Transportation of fish seed is a complex phenomenon associated with multiple kinds of stressors that simultaneously affect the fish in a confined environment, causing stress and mortality. The present study investigated the stress-relieving effect of exogenous glucose as a water additive in different concentrations (0.1, 0.2, 0.3, and 0.4%) during simulated transportation (12 h) of L. rohita fingerlings. The integrated biomarker response (IBR) index is a holistic tool to determine the optimum dose of exogenous glucose for mitigating transportation stress in fish. Based on selected biomarkers related to the stress hormone, serum biochemistry, oxidative stress, and HSP70 mRNA expression, the IBR index is calculated for each treatment and control group. The result showed a significant change in the level of stress hormone cortisol, enzymes (SGPT, LDH, MDH, SOD, CAT) and metabolites (serum glucose, triglyceride, creatinine) along with an upregulation in liver HSP70 mRNA expression. IBR index suggests that 0.2% glucose exhibited the lowest multi-biomarker stress response in comparison to other treatments and control. Therefore, the use of 0.2% glucose as a water additive will provide a solution to transportation induced stress in L. rohita fingerling and will underwrite the success of grow-out fish culture in days to come.https://doi.org/10.1038/s41598-021-85311-5 |
| spellingShingle | Abhilipsa Biswal Prem Prakash Srivastava Gopal Krishna Tapas Paul Prasenjit Pal Subodh Gupta Tincy Varghese Manish Jayant An Integrated biomarker approach for explaining the potency of exogenous glucose on transportation induced stress in Labeo rohita fingerlings Scientific Reports |
| title | An Integrated biomarker approach for explaining the potency of exogenous glucose on transportation induced stress in Labeo rohita fingerlings |
| title_full | An Integrated biomarker approach for explaining the potency of exogenous glucose on transportation induced stress in Labeo rohita fingerlings |
| title_fullStr | An Integrated biomarker approach for explaining the potency of exogenous glucose on transportation induced stress in Labeo rohita fingerlings |
| title_full_unstemmed | An Integrated biomarker approach for explaining the potency of exogenous glucose on transportation induced stress in Labeo rohita fingerlings |
| title_short | An Integrated biomarker approach for explaining the potency of exogenous glucose on transportation induced stress in Labeo rohita fingerlings |
| title_sort | integrated biomarker approach for explaining the potency of exogenous glucose on transportation induced stress in labeo rohita fingerlings |
| url | https://doi.org/10.1038/s41598-021-85311-5 |
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