Naturally-Acquired Immune Response against Plasmodium vivax Rhoptry-Associated Membrane Antigen.
Rhoptry-associated membrane antigen (RAMA) is an abundant glycophosphatidylinositol (GPI)-anchored protein that is embedded within the lipid bilayer and is implicated in parasite invasion. Antibody responses against rhoptry proteins are produced by individuals living in a malaria-endemic area, sugge...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2016-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4757550?pdf=render |
| _version_ | 1819291494640517120 |
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| author | Siriruk Changrob Bo Wang Jin-Hee Han Seong-Kyun Lee Myat Htut Nyunt Chae Seung Lim Takafumi Tsuboi Patchanee Chootong Eun-Taek Han |
| author_facet | Siriruk Changrob Bo Wang Jin-Hee Han Seong-Kyun Lee Myat Htut Nyunt Chae Seung Lim Takafumi Tsuboi Patchanee Chootong Eun-Taek Han |
| author_sort | Siriruk Changrob |
| collection | DOAJ |
| description | Rhoptry-associated membrane antigen (RAMA) is an abundant glycophosphatidylinositol (GPI)-anchored protein that is embedded within the lipid bilayer and is implicated in parasite invasion. Antibody responses against rhoptry proteins are produced by individuals living in a malaria-endemic area, suggesting the immunogenicity of Plasmodium vivax RAMA (PvRAMA) for induction of immune responses during P. vivax infection. To determine whether PvRAMA contributes to the acquisition of immunity to malaria and could be a rational candidate for a vaccine, the presence of memory T cells and the stability of the antibody response against PvRAMA were evaluated in P. vivax-exposed individuals. The immunogenicity of PvRAMA for the induction of T cell responses was evaluated by in vitro stimulation of peripheral blood mononuclear cells (PBMCs). High levels of interferon (IFN)-γ and interleukin (IL)-10 cytokines were detected in the culture supernatant of PBMCs, and the CD4+ T cells predominantly produced IL-10 cytokine. The levels of total anti-PvRAMA immunoglobulin G (IgG) antibody were significantly elevated, and these antibodies persisted over the 12 months of the study. Interestingly, IgG1, IgG2 and IgG3 were the major antibody subtypes in the response to PvRAMA. The frequency of IgG3 in specific to PvRAMA antigen maintained over 12 months. These data could explain the immunogenicity of PvRAMA antigen in induction of both cell-mediated and antibody-mediated immunity in natural P. vivax infection, in which IFN-γ helps antibody class switching toward the IgG1, IgG2 and IgG3 isotypes and IL-10 supports PvRAMA-specific antibody production. |
| first_indexed | 2024-12-24T03:39:32Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-24T03:39:32Z |
| publishDate | 2016-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-c07ba582cf61403c9799278bf64d1ca92022-12-21T17:16:57ZengPublic Library of Science (PLoS)PLoS ONE1932-62032016-01-01112e014872310.1371/journal.pone.0148723Naturally-Acquired Immune Response against Plasmodium vivax Rhoptry-Associated Membrane Antigen.Siriruk ChangrobBo WangJin-Hee HanSeong-Kyun LeeMyat Htut NyuntChae Seung LimTakafumi TsuboiPatchanee ChootongEun-Taek HanRhoptry-associated membrane antigen (RAMA) is an abundant glycophosphatidylinositol (GPI)-anchored protein that is embedded within the lipid bilayer and is implicated in parasite invasion. Antibody responses against rhoptry proteins are produced by individuals living in a malaria-endemic area, suggesting the immunogenicity of Plasmodium vivax RAMA (PvRAMA) for induction of immune responses during P. vivax infection. To determine whether PvRAMA contributes to the acquisition of immunity to malaria and could be a rational candidate for a vaccine, the presence of memory T cells and the stability of the antibody response against PvRAMA were evaluated in P. vivax-exposed individuals. The immunogenicity of PvRAMA for the induction of T cell responses was evaluated by in vitro stimulation of peripheral blood mononuclear cells (PBMCs). High levels of interferon (IFN)-γ and interleukin (IL)-10 cytokines were detected in the culture supernatant of PBMCs, and the CD4+ T cells predominantly produced IL-10 cytokine. The levels of total anti-PvRAMA immunoglobulin G (IgG) antibody were significantly elevated, and these antibodies persisted over the 12 months of the study. Interestingly, IgG1, IgG2 and IgG3 were the major antibody subtypes in the response to PvRAMA. The frequency of IgG3 in specific to PvRAMA antigen maintained over 12 months. These data could explain the immunogenicity of PvRAMA antigen in induction of both cell-mediated and antibody-mediated immunity in natural P. vivax infection, in which IFN-γ helps antibody class switching toward the IgG1, IgG2 and IgG3 isotypes and IL-10 supports PvRAMA-specific antibody production.http://europepmc.org/articles/PMC4757550?pdf=render |
| spellingShingle | Siriruk Changrob Bo Wang Jin-Hee Han Seong-Kyun Lee Myat Htut Nyunt Chae Seung Lim Takafumi Tsuboi Patchanee Chootong Eun-Taek Han Naturally-Acquired Immune Response against Plasmodium vivax Rhoptry-Associated Membrane Antigen. PLoS ONE |
| title | Naturally-Acquired Immune Response against Plasmodium vivax Rhoptry-Associated Membrane Antigen. |
| title_full | Naturally-Acquired Immune Response against Plasmodium vivax Rhoptry-Associated Membrane Antigen. |
| title_fullStr | Naturally-Acquired Immune Response against Plasmodium vivax Rhoptry-Associated Membrane Antigen. |
| title_full_unstemmed | Naturally-Acquired Immune Response against Plasmodium vivax Rhoptry-Associated Membrane Antigen. |
| title_short | Naturally-Acquired Immune Response against Plasmodium vivax Rhoptry-Associated Membrane Antigen. |
| title_sort | naturally acquired immune response against plasmodium vivax rhoptry associated membrane antigen |
| url | http://europepmc.org/articles/PMC4757550?pdf=render |
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