Single-cell manipulation by two-dimensional micropatterning
Cells are highly sensitive to their geometrical and mechanical microenvironment that directly regulate cell shape, cytoskeleton and organelle, as well as the nucleus morphology and genetic expression. The emerging two-dimensional micropatterning techniques offer powerful tools to construct controlla...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
World Scientific Publishing
2024-01-01
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| Series: | Journal of Innovative Optical Health Sciences |
| Subjects: | |
| Online Access: | https://www.worldscientific.com/doi/10.1142/S1793545823300112 |
| _version_ | 1827367959689953280 |
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| author | Xuehe Ma Haimei Zhang Shiyu Deng Qiushuo Sun Qingsong Hu Yuhang Pan Fen Hu Imshik Lee Fulin Xing Leiting Pan |
| author_facet | Xuehe Ma Haimei Zhang Shiyu Deng Qiushuo Sun Qingsong Hu Yuhang Pan Fen Hu Imshik Lee Fulin Xing Leiting Pan |
| author_sort | Xuehe Ma |
| collection | DOAJ |
| description | Cells are highly sensitive to their geometrical and mechanical microenvironment that directly regulate cell shape, cytoskeleton and organelle, as well as the nucleus morphology and genetic expression. The emerging two-dimensional micropatterning techniques offer powerful tools to construct controllable and well-organized microenvironment for single-cell level investigations with qualitative analysis, cellular standardization, and in vivo environment mimicking. Here, we provide an overview of the basic principle and characteristics of the two most widely-used micropatterning techniques, including photolithographic micropatterning and soft lithography micropatterning. Moreover, we summarize the application of micropatterning technique in controlling cytoskeleton, cell migration, nucleus and gene expression, as well as intercellular communication. |
| first_indexed | 2024-03-08T09:23:19Z |
| format | Article |
| id | doaj.art-c07d1573cbf64a25a610bf550d0282f8 |
| institution | Directory Open Access Journal |
| issn | 1793-5458 1793-7205 |
| language | English |
| last_indexed | 2024-03-08T09:23:19Z |
| publishDate | 2024-01-01 |
| publisher | World Scientific Publishing |
| record_format | Article |
| series | Journal of Innovative Optical Health Sciences |
| spelling | doaj.art-c07d1573cbf64a25a610bf550d0282f82024-01-31T11:09:17ZengWorld Scientific PublishingJournal of Innovative Optical Health Sciences1793-54581793-72052024-01-01170110.1142/S1793545823300112Single-cell manipulation by two-dimensional micropatterningXuehe Ma0Haimei Zhang1Shiyu Deng2Qiushuo Sun3Qingsong Hu4Yuhang Pan5Fen Hu6Imshik Lee7Fulin Xing8Leiting Pan9The Key Laboratory of Weak-Light Nonlinear, Photonics of Education Ministry, School of Physics and TEDA Institute of Applied Physics, Nankai University, Tianjin 300071, P. R. ChinaThe Key Laboratory of Weak-Light Nonlinear, Photonics of Education Ministry, School of Physics and TEDA Institute of Applied Physics, Nankai University, Tianjin 300071, P. R. ChinaThe Key Laboratory of Weak-Light Nonlinear, Photonics of Education Ministry, School of Physics and TEDA Institute of Applied Physics, Nankai University, Tianjin 300071, P. R. ChinaThe Key Laboratory of Weak-Light Nonlinear, Photonics of Education Ministry, School of Physics and TEDA Institute of Applied Physics, Nankai University, Tianjin 300071, P. R. ChinaThe Key Laboratory of Weak-Light Nonlinear, Photonics of Education Ministry, School of Physics and TEDA Institute of Applied Physics, Nankai University, Tianjin 300071, P. R. ChinaThe Key Laboratory of Weak-Light Nonlinear, Photonics of Education Ministry, School of Physics and TEDA Institute of Applied Physics, Nankai University, Tianjin 300071, P. R. ChinaThe Key Laboratory of Weak-Light Nonlinear, Photonics of Education Ministry, School of Physics and TEDA Institute of Applied Physics, Nankai University, Tianjin 300071, P. R. ChinaThe Key Laboratory of Weak-Light Nonlinear, Photonics of Education Ministry, School of Physics and TEDA Institute of Applied Physics, Nankai University, Tianjin 300071, P. R. ChinaThe Key Laboratory of Weak-Light Nonlinear, Photonics of Education Ministry, School of Physics and TEDA Institute of Applied Physics, Nankai University, Tianjin 300071, P. R. ChinaThe Key Laboratory of Weak-Light Nonlinear, Photonics of Education Ministry, School of Physics and TEDA Institute of Applied Physics, Nankai University, Tianjin 300071, P. R. ChinaCells are highly sensitive to their geometrical and mechanical microenvironment that directly regulate cell shape, cytoskeleton and organelle, as well as the nucleus morphology and genetic expression. The emerging two-dimensional micropatterning techniques offer powerful tools to construct controllable and well-organized microenvironment for single-cell level investigations with qualitative analysis, cellular standardization, and in vivo environment mimicking. Here, we provide an overview of the basic principle and characteristics of the two most widely-used micropatterning techniques, including photolithographic micropatterning and soft lithography micropatterning. Moreover, we summarize the application of micropatterning technique in controlling cytoskeleton, cell migration, nucleus and gene expression, as well as intercellular communication.https://www.worldscientific.com/doi/10.1142/S1793545823300112Two-dimensional micropatterningcytoskeletoncell migrationextracellular matrixintercellular communicationgene expression |
| spellingShingle | Xuehe Ma Haimei Zhang Shiyu Deng Qiushuo Sun Qingsong Hu Yuhang Pan Fen Hu Imshik Lee Fulin Xing Leiting Pan Single-cell manipulation by two-dimensional micropatterning Journal of Innovative Optical Health Sciences Two-dimensional micropatterning cytoskeleton cell migration extracellular matrix intercellular communication gene expression |
| title | Single-cell manipulation by two-dimensional micropatterning |
| title_full | Single-cell manipulation by two-dimensional micropatterning |
| title_fullStr | Single-cell manipulation by two-dimensional micropatterning |
| title_full_unstemmed | Single-cell manipulation by two-dimensional micropatterning |
| title_short | Single-cell manipulation by two-dimensional micropatterning |
| title_sort | single cell manipulation by two dimensional micropatterning |
| topic | Two-dimensional micropatterning cytoskeleton cell migration extracellular matrix intercellular communication gene expression |
| url | https://www.worldscientific.com/doi/10.1142/S1793545823300112 |
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