Effect of 8 weeks high-intensity interval training (HIIT) with and without caloric restriction on gene expression of myocardial Bax and Bcl2 in mice
Background: Apoptosis is the physiological cell death that in natural conditions leads to the elimination of old, damaged, waste, and harmful cells. The aim of this study was the effect of eight weeks of high-intensity interval training (HIIT) with and without caloric restriction on gene expression...
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| Format: | Article |
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Tabriz University of Medical Sciences
2021-04-01
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| Series: | مجله پزشکی دانشگاه علوم پزشکی تبریز |
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| Online Access: | https://mj.tbzmed.ac.ir/PDF/mj-43-1-124.pdf |
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| author | Mohammadreza Zarali Zaher Etemad Kamal Azizbeigi Pouran Karimi |
| author_facet | Mohammadreza Zarali Zaher Etemad Kamal Azizbeigi Pouran Karimi |
| author_sort | Mohammadreza Zarali |
| collection | DOAJ |
| description | Background: Apoptosis is the physiological cell death that in natural conditions leads to the elimination of old, damaged, waste, and harmful cells. The aim of this study was the effect of eight weeks of high-intensity interval training (HIIT) with and without caloric restriction on gene expression of myocardial Bax and Bcl2 in mice. Methods: Present study was an experimental multi-group design with a control group conducted on 30 two-month old male mice. Subjects were divided into five homogenous groups including base control, control, caloric restriction, interval exercise training, and caloric restriction + interval exercise training. Training groups participated in interval exercise training five sessions per week for 8 weeks. The level of gene expression of myocardial Bax and Bcl2 was evaluated by real-time PCR. Data were analyzed using the one-way ANOVA at the level of (P<0.05). Results: The results showed that the training group had a significant increase in gene expression of myocardial Bcl2 in comparison with caloric restriction + exercise training (P<0.05) and a significant decrease in gene expression of myocardial Bax compared to the caloric restriction group (P<0.05). Also, exercise training and exercise training + caloric restriction significantly increased the gene expression of myocardial Bcl2 and significantly decreased Bax/Bcl2 ratio compared to caloric restriction, base control, and control (P< 0.05). Conclusion: It seems that high-intensity interval training without caloric restriction would provide a suitable environment for increasing the integrity of the mitochondrial membrane of myocardial cells and possibly apoptosis. |
| first_indexed | 2024-04-11T08:07:22Z |
| format | Article |
| id | doaj.art-c07d6efb7483478cade5eb5e1f611a9f |
| institution | Directory Open Access Journal |
| issn | 2783-2031 2783-204X |
| language | fas |
| last_indexed | 2024-04-11T08:07:22Z |
| publishDate | 2021-04-01 |
| publisher | Tabriz University of Medical Sciences |
| record_format | Article |
| series | مجله پزشکی دانشگاه علوم پزشکی تبریز |
| spelling | doaj.art-c07d6efb7483478cade5eb5e1f611a9f2022-12-22T04:35:29ZfasTabriz University of Medical Sciencesمجله پزشکی دانشگاه علوم پزشکی تبریز2783-20312783-204X2021-04-0143112413310.34172/mj.2021.03727184Effect of 8 weeks high-intensity interval training (HIIT) with and without caloric restriction on gene expression of myocardial Bax and Bcl2 in miceMohammadreza Zarali0Zaher Etemad1Kamal Azizbeigi2Pouran Karimi3PhD Student in Sports Physiology, Sanandaj Branch, Islamic Azad University, Sanandaj, IranDepartment of Physical Education, Sanandaj Branch, Islamic Azad University, Sanandaj, IranDepartment of Physical Education, Sanandaj Branch, Islamic Azad University, Sanandaj, IranDepartment of Clinical Biochemistry, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, IranBackground: Apoptosis is the physiological cell death that in natural conditions leads to the elimination of old, damaged, waste, and harmful cells. The aim of this study was the effect of eight weeks of high-intensity interval training (HIIT) with and without caloric restriction on gene expression of myocardial Bax and Bcl2 in mice. Methods: Present study was an experimental multi-group design with a control group conducted on 30 two-month old male mice. Subjects were divided into five homogenous groups including base control, control, caloric restriction, interval exercise training, and caloric restriction + interval exercise training. Training groups participated in interval exercise training five sessions per week for 8 weeks. The level of gene expression of myocardial Bax and Bcl2 was evaluated by real-time PCR. Data were analyzed using the one-way ANOVA at the level of (P<0.05). Results: The results showed that the training group had a significant increase in gene expression of myocardial Bcl2 in comparison with caloric restriction + exercise training (P<0.05) and a significant decrease in gene expression of myocardial Bax compared to the caloric restriction group (P<0.05). Also, exercise training and exercise training + caloric restriction significantly increased the gene expression of myocardial Bcl2 and significantly decreased Bax/Bcl2 ratio compared to caloric restriction, base control, and control (P< 0.05). Conclusion: It seems that high-intensity interval training without caloric restriction would provide a suitable environment for increasing the integrity of the mitochondrial membrane of myocardial cells and possibly apoptosis.https://mj.tbzmed.ac.ir/PDF/mj-43-1-124.pdfhigh-intensity interval trainingcaloric restrictionbaxbcl2 |
| spellingShingle | Mohammadreza Zarali Zaher Etemad Kamal Azizbeigi Pouran Karimi Effect of 8 weeks high-intensity interval training (HIIT) with and without caloric restriction on gene expression of myocardial Bax and Bcl2 in mice مجله پزشکی دانشگاه علوم پزشکی تبریز high-intensity interval training caloric restriction bax bcl2 |
| title | Effect of 8 weeks high-intensity interval training (HIIT) with and without caloric restriction on gene expression of myocardial Bax and Bcl2 in mice |
| title_full | Effect of 8 weeks high-intensity interval training (HIIT) with and without caloric restriction on gene expression of myocardial Bax and Bcl2 in mice |
| title_fullStr | Effect of 8 weeks high-intensity interval training (HIIT) with and without caloric restriction on gene expression of myocardial Bax and Bcl2 in mice |
| title_full_unstemmed | Effect of 8 weeks high-intensity interval training (HIIT) with and without caloric restriction on gene expression of myocardial Bax and Bcl2 in mice |
| title_short | Effect of 8 weeks high-intensity interval training (HIIT) with and without caloric restriction on gene expression of myocardial Bax and Bcl2 in mice |
| title_sort | effect of 8 weeks high intensity interval training hiit with and without caloric restriction on gene expression of myocardial bax and bcl2 in mice |
| topic | high-intensity interval training caloric restriction bax bcl2 |
| url | https://mj.tbzmed.ac.ir/PDF/mj-43-1-124.pdf |
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