Efficient Plant Regeneration System from Leaf Explant Cultures of <i>Daphne genkwa</i> via Somatic Embryogenesis
This study aimed to establish an efficient plant regeneration system from leaf-derived embryogenic structure cultures of <i>Daphne genkwa</i>. To induce embryogenic structures, fully expanded leaf explants of <i>D. genkwa</i> were cultured on Murashige and Skoog (MS) medium s...
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MDPI AG
2023-05-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/12/11/2175 |
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| author | Seong Sub Ku Hyun-A Woo Min Jun Shin Eun Yee Jie HyeRan Kim Hyun-Soon Kim Hye Sun Cho Won-Joong Jeong Moon-Soon Lee Sung Ran Min Suk Weon Kim |
| author_facet | Seong Sub Ku Hyun-A Woo Min Jun Shin Eun Yee Jie HyeRan Kim Hyun-Soon Kim Hye Sun Cho Won-Joong Jeong Moon-Soon Lee Sung Ran Min Suk Weon Kim |
| author_sort | Seong Sub Ku |
| collection | DOAJ |
| description | This study aimed to establish an efficient plant regeneration system from leaf-derived embryogenic structure cultures of <i>Daphne genkwa</i>. To induce embryogenic structures, fully expanded leaf explants of <i>D. genkwa</i> were cultured on Murashige and Skoog (MS) medium supplemented with 0, 0.1, 0.5, 1, 2, and 5 mg·L<sup>−1</sup> 2,4-dichlorophenoxyacetic acid (2,4-D), respectively. After 8 weeks of incubation, the highest frequency of embryogenic structure formation reached 100% when the leaf explants were cultivated on MS medium supplemented with 0.1 to 1 mg·L<sup>−1</sup> 2,4-D. At higher concentrations of 2,4-D (over 2 mg·L<sup>−1</sup> 2,4-D), the frequency of embryogenic structure formation significantly declined. Similar to 2,4-D, indole butyric acid (IBA) and α-naphthaleneacetic acid (NAA) treatments were also able to form embryogenic structures. However, the frequency of embryogenic structure formation was lower than that of 2,4-D. In particular, the yellow embryonic structure (YES) and white embryonic structure (WES) were simultaneously developed from the leaf explants of <i>D. genkwa</i> on culture medium containing 2,4-D, IBA, and NAA, respectively. Embryogenic calluses (ECs) were formed from the YES after subsequent rounds of subculture on MS medium supplemented with 1 mg·L<sup>−1</sup> 2,4-D. To regenerate whole plants, the embryogenic callus (EC) and the two embryogenic structures (YES and WES) were transferred onto MS medium supplemented with 0.1 mg·L<sup>−1</sup> 6-benzyl aminopurine (BA). The YES had the highest plant regeneration potential via somatic embryo and shoot development compared to the EC and WES. To our knowledge, this is the first successful report of a plant regeneration system via the somatic embryogenesis of <i>D. genkwa</i>. Thus, the embryogenic structures and plant regeneration system of <i>D. genkwa</i> could be applied to mass proliferation and genetic modification for pharmaceutical metabolite production in <i>D. genkwa</i>. |
| first_indexed | 2024-03-11T03:00:02Z |
| format | Article |
| id | doaj.art-c07f8e36e41742839d2cc4d25e15145f |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-11T03:00:02Z |
| publishDate | 2023-05-01 |
| publisher | MDPI AG |
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| spelling | doaj.art-c07f8e36e41742839d2cc4d25e15145f2023-11-18T08:24:21ZengMDPI AGPlants2223-77472023-05-011211217510.3390/plants12112175Efficient Plant Regeneration System from Leaf Explant Cultures of <i>Daphne genkwa</i> via Somatic EmbryogenesisSeong Sub Ku0Hyun-A Woo1Min Jun Shin2Eun Yee Jie3HyeRan Kim4Hyun-Soon Kim5Hye Sun Cho6Won-Joong Jeong7Moon-Soon Lee8Sung Ran Min9Suk Weon Kim10Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 34141, Republic of KoreaPlant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 34141, Republic of KoreaPlant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 34141, Republic of KoreaBiological Resource Center, Korea Research Institute of Bioscience and Biotechnology, Jeongeup 56212, Republic of KoreaPlant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 34141, Republic of KoreaPlant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 34141, Republic of KoreaPlant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 34141, Republic of KoreaPlant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 34141, Republic of KoreaDepartment of Industrial Plant Science and Technology, Chungbuk National University, Cheongju 28644, Republic of KoreaPlant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 34141, Republic of KoreaBiological Resource Center, Korea Research Institute of Bioscience and Biotechnology, Jeongeup 56212, Republic of KoreaThis study aimed to establish an efficient plant regeneration system from leaf-derived embryogenic structure cultures of <i>Daphne genkwa</i>. To induce embryogenic structures, fully expanded leaf explants of <i>D. genkwa</i> were cultured on Murashige and Skoog (MS) medium supplemented with 0, 0.1, 0.5, 1, 2, and 5 mg·L<sup>−1</sup> 2,4-dichlorophenoxyacetic acid (2,4-D), respectively. After 8 weeks of incubation, the highest frequency of embryogenic structure formation reached 100% when the leaf explants were cultivated on MS medium supplemented with 0.1 to 1 mg·L<sup>−1</sup> 2,4-D. At higher concentrations of 2,4-D (over 2 mg·L<sup>−1</sup> 2,4-D), the frequency of embryogenic structure formation significantly declined. Similar to 2,4-D, indole butyric acid (IBA) and α-naphthaleneacetic acid (NAA) treatments were also able to form embryogenic structures. However, the frequency of embryogenic structure formation was lower than that of 2,4-D. In particular, the yellow embryonic structure (YES) and white embryonic structure (WES) were simultaneously developed from the leaf explants of <i>D. genkwa</i> on culture medium containing 2,4-D, IBA, and NAA, respectively. Embryogenic calluses (ECs) were formed from the YES after subsequent rounds of subculture on MS medium supplemented with 1 mg·L<sup>−1</sup> 2,4-D. To regenerate whole plants, the embryogenic callus (EC) and the two embryogenic structures (YES and WES) were transferred onto MS medium supplemented with 0.1 mg·L<sup>−1</sup> 6-benzyl aminopurine (BA). The YES had the highest plant regeneration potential via somatic embryo and shoot development compared to the EC and WES. To our knowledge, this is the first successful report of a plant regeneration system via the somatic embryogenesis of <i>D. genkwa</i>. Thus, the embryogenic structures and plant regeneration system of <i>D. genkwa</i> could be applied to mass proliferation and genetic modification for pharmaceutical metabolite production in <i>D. genkwa</i>.https://www.mdpi.com/2223-7747/12/11/2175auxinembryogenic structuremedicinal plantsomatic embryo |
| spellingShingle | Seong Sub Ku Hyun-A Woo Min Jun Shin Eun Yee Jie HyeRan Kim Hyun-Soon Kim Hye Sun Cho Won-Joong Jeong Moon-Soon Lee Sung Ran Min Suk Weon Kim Efficient Plant Regeneration System from Leaf Explant Cultures of <i>Daphne genkwa</i> via Somatic Embryogenesis Plants auxin embryogenic structure medicinal plant somatic embryo |
| title | Efficient Plant Regeneration System from Leaf Explant Cultures of <i>Daphne genkwa</i> via Somatic Embryogenesis |
| title_full | Efficient Plant Regeneration System from Leaf Explant Cultures of <i>Daphne genkwa</i> via Somatic Embryogenesis |
| title_fullStr | Efficient Plant Regeneration System from Leaf Explant Cultures of <i>Daphne genkwa</i> via Somatic Embryogenesis |
| title_full_unstemmed | Efficient Plant Regeneration System from Leaf Explant Cultures of <i>Daphne genkwa</i> via Somatic Embryogenesis |
| title_short | Efficient Plant Regeneration System from Leaf Explant Cultures of <i>Daphne genkwa</i> via Somatic Embryogenesis |
| title_sort | efficient plant regeneration system from leaf explant cultures of i daphne genkwa i via somatic embryogenesis |
| topic | auxin embryogenic structure medicinal plant somatic embryo |
| url | https://www.mdpi.com/2223-7747/12/11/2175 |
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