Development of a Polymersome-Based Nanomedicine for Chemotherapeutic and Sonodynamic Combination Therapy
In anticancer therapy, combination therapy has been suggested as an alternative to the insufficient therapeutic efficacy of single therapy. Among combination therapies, combination chemo- and photodynamic therapy are actively investigated. However, photodynamic therapy shows a limitation in the pene...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-01-01
|
| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/24/2/1194 |
| _version_ | 1797441507879288832 |
|---|---|
| author | Mingyun Kim Doyeon Kim Yongho Jang Hyounkoo Han Seonock Lee Hyungwon Moon Jungho Kim Hyuncheol Kim |
| author_facet | Mingyun Kim Doyeon Kim Yongho Jang Hyounkoo Han Seonock Lee Hyungwon Moon Jungho Kim Hyuncheol Kim |
| author_sort | Mingyun Kim |
| collection | DOAJ |
| description | In anticancer therapy, combination therapy has been suggested as an alternative to the insufficient therapeutic efficacy of single therapy. Among combination therapies, combination chemo- and photodynamic therapy are actively investigated. However, photodynamic therapy shows a limitation in the penetration depth of the laser. Therefore, sonodynamic therapy (SDT), using ultrasound instead of a laser as a trigger, is an upcoming strategy for deep tumors. Additionally, free drugs are easily degraded by enzymes, have difficulty in reaching the target site, and show side effects after systemic administration; therefore, the development of drug delivery systems is desirable for sufficient drug efficacy for combination therapy. However, nanocarriers, such as microbubbles, and albumin nanoparticles, are unstable in the body and show low drug-loading efficiency. Here, we propose polylactide (PLA)-poly (ethylene glycol) (PEG) polymersomes (PLs) with a high drug loading rate of doxorubicin (DOX) and verteporfin (VP) for effective combination therapy in both in vitro and in vivo experiments. The cellular uptake efficiency and cytotoxicity test results of VP-DOX-PLs were higher than that of single therapy. Moreover, in vivo biodistribution showed the accumulation of the VP-DOX-PLs in tumor regions. Therefore, VP-DOX-PLs showed more effective anticancer efficacy than either single therapy in vivo. These results suggest that the combination therapy of SDT and chemotherapy could show novel anticancer effects using VP-DOX-PLs. |
| first_indexed | 2024-03-09T12:25:09Z |
| format | Article |
| id | doaj.art-c082e7948bd144a8b65b0c60ee49b9bd |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T12:25:09Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-c082e7948bd144a8b65b0c60ee49b9bd2023-11-30T22:36:46ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-01-01242119410.3390/ijms24021194Development of a Polymersome-Based Nanomedicine for Chemotherapeutic and Sonodynamic Combination TherapyMingyun Kim0Doyeon Kim1Yongho Jang2Hyounkoo Han3Seonock Lee4Hyungwon Moon5Jungho Kim6Hyuncheol Kim7Department of Chemical and Biomolecular Engineering, Sogang University, 35 Baekbeom-ro, Mapo-gu, Seoul 04107, Republic of KoreaDepartment of Chemical and Biomolecular Engineering, Sogang University, 35 Baekbeom-ro, Mapo-gu, Seoul 04107, Republic of KoreaDepartment of Chemical and Biomolecular Engineering, Sogang University, 35 Baekbeom-ro, Mapo-gu, Seoul 04107, Republic of KoreaDepartment of Chemical and Biomolecular Engineering, Sogang University, 35 Baekbeom-ro, Mapo-gu, Seoul 04107, Republic of KoreaDepartment of Life Science, Sogang University, 35 Baekbeom-ro, Mapo-gu, Seoul 04107, Republic of KoreaR&D Center, IMGT Co., Ltd., Seongnam-si 13605, Republic of KoreaDepartment of Life Science, Sogang University, 35 Baekbeom-ro, Mapo-gu, Seoul 04107, Republic of KoreaDepartment of Chemical and Biomolecular Engineering, Sogang University, 35 Baekbeom-ro, Mapo-gu, Seoul 04107, Republic of KoreaIn anticancer therapy, combination therapy has been suggested as an alternative to the insufficient therapeutic efficacy of single therapy. Among combination therapies, combination chemo- and photodynamic therapy are actively investigated. However, photodynamic therapy shows a limitation in the penetration depth of the laser. Therefore, sonodynamic therapy (SDT), using ultrasound instead of a laser as a trigger, is an upcoming strategy for deep tumors. Additionally, free drugs are easily degraded by enzymes, have difficulty in reaching the target site, and show side effects after systemic administration; therefore, the development of drug delivery systems is desirable for sufficient drug efficacy for combination therapy. However, nanocarriers, such as microbubbles, and albumin nanoparticles, are unstable in the body and show low drug-loading efficiency. Here, we propose polylactide (PLA)-poly (ethylene glycol) (PEG) polymersomes (PLs) with a high drug loading rate of doxorubicin (DOX) and verteporfin (VP) for effective combination therapy in both in vitro and in vivo experiments. The cellular uptake efficiency and cytotoxicity test results of VP-DOX-PLs were higher than that of single therapy. Moreover, in vivo biodistribution showed the accumulation of the VP-DOX-PLs in tumor regions. Therefore, VP-DOX-PLs showed more effective anticancer efficacy than either single therapy in vivo. These results suggest that the combination therapy of SDT and chemotherapy could show novel anticancer effects using VP-DOX-PLs.https://www.mdpi.com/1422-0067/24/2/1194sonodynamic therapycombination therapypolymersomesdrug deliverydoxorubicinverteporfin |
| spellingShingle | Mingyun Kim Doyeon Kim Yongho Jang Hyounkoo Han Seonock Lee Hyungwon Moon Jungho Kim Hyuncheol Kim Development of a Polymersome-Based Nanomedicine for Chemotherapeutic and Sonodynamic Combination Therapy International Journal of Molecular Sciences sonodynamic therapy combination therapy polymersomes drug delivery doxorubicin verteporfin |
| title | Development of a Polymersome-Based Nanomedicine for Chemotherapeutic and Sonodynamic Combination Therapy |
| title_full | Development of a Polymersome-Based Nanomedicine for Chemotherapeutic and Sonodynamic Combination Therapy |
| title_fullStr | Development of a Polymersome-Based Nanomedicine for Chemotherapeutic and Sonodynamic Combination Therapy |
| title_full_unstemmed | Development of a Polymersome-Based Nanomedicine for Chemotherapeutic and Sonodynamic Combination Therapy |
| title_short | Development of a Polymersome-Based Nanomedicine for Chemotherapeutic and Sonodynamic Combination Therapy |
| title_sort | development of a polymersome based nanomedicine for chemotherapeutic and sonodynamic combination therapy |
| topic | sonodynamic therapy combination therapy polymersomes drug delivery doxorubicin verteporfin |
| url | https://www.mdpi.com/1422-0067/24/2/1194 |
| work_keys_str_mv | AT mingyunkim developmentofapolymersomebasednanomedicineforchemotherapeuticandsonodynamiccombinationtherapy AT doyeonkim developmentofapolymersomebasednanomedicineforchemotherapeuticandsonodynamiccombinationtherapy AT yonghojang developmentofapolymersomebasednanomedicineforchemotherapeuticandsonodynamiccombinationtherapy AT hyounkoohan developmentofapolymersomebasednanomedicineforchemotherapeuticandsonodynamiccombinationtherapy AT seonocklee developmentofapolymersomebasednanomedicineforchemotherapeuticandsonodynamiccombinationtherapy AT hyungwonmoon developmentofapolymersomebasednanomedicineforchemotherapeuticandsonodynamiccombinationtherapy AT junghokim developmentofapolymersomebasednanomedicineforchemotherapeuticandsonodynamiccombinationtherapy AT hyuncheolkim developmentofapolymersomebasednanomedicineforchemotherapeuticandsonodynamiccombinationtherapy |