EFFECT OF TEMPERATURE ON THE VIABILITY OF PLANKTON CELLS AND MODEL LEGIONELLA PNEUMOPHILA BIOFILMS IN WATER
Aim. Study the effect of water temperature from 40 to 70°C on viability of plankton forms and model Legionella pneumophila under experimental conditions. Materials and methods. Monospecies legionella biofilms, obtained in plates for enzyme immunoassay during 96 hours at 28°C, and water suspension of...
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| Format: | Article |
| Language: | Russian |
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Central Research Institute for Epidemiology
2015-10-01
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| Series: | Журнал микробиологии, эпидемиологии и иммунобиологии |
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| Online Access: | https://microbiol.crie.ru/jour/article/view/14110 |
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| author | I. S Tartakovsky T. I Karpova O. A Gruzdeva O. V Marinenko Yu. E Dronina |
| author_facet | I. S Tartakovsky T. I Karpova O. A Gruzdeva O. V Marinenko Yu. E Dronina |
| author_sort | I. S Tartakovsky |
| collection | DOAJ |
| description | Aim. Study the effect of water temperature from 40 to 70°C on viability of plankton forms and model Legionella pneumophila under experimental conditions. Materials and methods. Monospecies legionella biofilms, obtained in plates for enzyme immunoassay during 96 hours at 28°C, and water suspension of BCYE agar cultivated cells of L.pneumophila at a concentration of 103 - 105 CFU per liter were used in the study for evaluation of bactericidal effect of temperature on various legionella forms. Results. Analysis of effects of various temperature regimens on plankton forms and model legionella biofilms has shown that at a temperature range from 50 to 60°C a significant reduction of quantity of viable legionella cells occurs. Model legionella biofilms have partially conserved viability at a temperature of 60°C and only exposition to a temperature of 70°C resulted in death of legionella biofilms and plankton forms of bacteria. A dependence of viability conservation of legionella from the initial concentration of the causative agent in water and duration of exposition at varying temperature was shown. Conclusion. Short-term heating at a temperature of at least 70°C has the most pronounced bactericidal effect on plankton forms and model L.pneumophila biofilms under experimental conditions. Such temperature regimen could be used as one of the prophylaxis approaches during maintenance of especially dangerous water system and, fist of all, systems of hot water supply. |
| first_indexed | 2024-03-13T00:08:03Z |
| format | Article |
| id | doaj.art-c0830abe406747af87001800cf72e9e4 |
| institution | Directory Open Access Journal |
| issn | 0372-9311 2686-7613 |
| language | Russian |
| last_indexed | 2024-03-13T00:08:03Z |
| publishDate | 2015-10-01 |
| publisher | Central Research Institute for Epidemiology |
| record_format | Article |
| series | Журнал микробиологии, эпидемиологии и иммунобиологии |
| spelling | doaj.art-c0830abe406747af87001800cf72e9e42023-07-12T20:19:07ZrusCentral Research Institute for EpidemiologyЖурнал микробиологии, эпидемиологии и иммунобиологии0372-93112686-76132015-10-019257122535EFFECT OF TEMPERATURE ON THE VIABILITY OF PLANKTON CELLS AND MODEL LEGIONELLA PNEUMOPHILA BIOFILMS IN WATERI. S Tartakovsky0T. I Karpova1O. A Gruzdeva2O. V Marinenko3Yu. E Dronina4Sechenov First Moscow State Medical UniversitySechenov First Moscow State Medical UniversityGamaleya Federal Research Centre of Epidemiology and MicrobiologySechenov First Moscow State Medical UniversitySechenov First Moscow State Medical UniversityAim. Study the effect of water temperature from 40 to 70°C on viability of plankton forms and model Legionella pneumophila under experimental conditions. Materials and methods. Monospecies legionella biofilms, obtained in plates for enzyme immunoassay during 96 hours at 28°C, and water suspension of BCYE agar cultivated cells of L.pneumophila at a concentration of 103 - 105 CFU per liter were used in the study for evaluation of bactericidal effect of temperature on various legionella forms. Results. Analysis of effects of various temperature regimens on plankton forms and model legionella biofilms has shown that at a temperature range from 50 to 60°C a significant reduction of quantity of viable legionella cells occurs. Model legionella biofilms have partially conserved viability at a temperature of 60°C and only exposition to a temperature of 70°C resulted in death of legionella biofilms and plankton forms of bacteria. A dependence of viability conservation of legionella from the initial concentration of the causative agent in water and duration of exposition at varying temperature was shown. Conclusion. Short-term heating at a temperature of at least 70°C has the most pronounced bactericidal effect on plankton forms and model L.pneumophila biofilms under experimental conditions. Such temperature regimen could be used as one of the prophylaxis approaches during maintenance of especially dangerous water system and, fist of all, systems of hot water supply.https://microbiol.crie.ru/jour/article/view/14110l.pneumophilal.pneumophilabiofilmstemperaturewaterprophylaxis |
| spellingShingle | I. S Tartakovsky T. I Karpova O. A Gruzdeva O. V Marinenko Yu. E Dronina EFFECT OF TEMPERATURE ON THE VIABILITY OF PLANKTON CELLS AND MODEL LEGIONELLA PNEUMOPHILA BIOFILMS IN WATER Журнал микробиологии, эпидемиологии и иммунобиологии l.pneumophila l.pneumophila biofilms temperature water prophylaxis |
| title | EFFECT OF TEMPERATURE ON THE VIABILITY OF PLANKTON CELLS AND MODEL LEGIONELLA PNEUMOPHILA BIOFILMS IN WATER |
| title_full | EFFECT OF TEMPERATURE ON THE VIABILITY OF PLANKTON CELLS AND MODEL LEGIONELLA PNEUMOPHILA BIOFILMS IN WATER |
| title_fullStr | EFFECT OF TEMPERATURE ON THE VIABILITY OF PLANKTON CELLS AND MODEL LEGIONELLA PNEUMOPHILA BIOFILMS IN WATER |
| title_full_unstemmed | EFFECT OF TEMPERATURE ON THE VIABILITY OF PLANKTON CELLS AND MODEL LEGIONELLA PNEUMOPHILA BIOFILMS IN WATER |
| title_short | EFFECT OF TEMPERATURE ON THE VIABILITY OF PLANKTON CELLS AND MODEL LEGIONELLA PNEUMOPHILA BIOFILMS IN WATER |
| title_sort | effect of temperature on the viability of plankton cells and model legionella pneumophila biofilms in water |
| topic | l.pneumophila l.pneumophila biofilms temperature water prophylaxis |
| url | https://microbiol.crie.ru/jour/article/view/14110 |
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