Antimicrobial and antioxidant properties of methanol extract, fractions and compounds from the stem bark of <it>Entada abyssinica </it>Stend ex A. Satabie
<p>Abstract</p> <p>Background</p> <p>The aim of this study was to evaluate the antimicrobial and antioxidant activities of the methanol extract, fractions and isolated compounds from <it>Entada abyssinica </it>stem bark, plant used traditionally against gast...
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| Format: | Article |
| Language: | English |
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BMC
2011-07-01
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| Series: | BMC Complementary and Alternative Medicine |
| Online Access: | http://www.biomedcentral.com/1472-6882/11/57 |
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| author | Giacinti Geraldine Vilarem Gerard Kuiate Jules-Roger Wabo Hippolyte K Lunga Paul K Teke Gerald N Kikuchi Haruhisa Oshima Yoshiteru |
| author_facet | Giacinti Geraldine Vilarem Gerard Kuiate Jules-Roger Wabo Hippolyte K Lunga Paul K Teke Gerald N Kikuchi Haruhisa Oshima Yoshiteru |
| author_sort | Giacinti Geraldine |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>The aim of this study was to evaluate the antimicrobial and antioxidant activities of the methanol extract, fractions and isolated compounds from <it>Entada abyssinica </it>stem bark, plant used traditionally against gastrointestinal infections.</p> <p>Methods</p> <p>The methanol extract of <it>E. abyssinica </it>stem bark was pre-dissolved in a mixture of methanol and water, and then partitioned between <it>n</it>-hexane, ethyl acetate and <it>n</it>-butanol. The ethyl acetate portion was fractionated by column chromatography and the structures of isolated compounds elucidated by analysis of spectroscopic data and comparison with literature data. Antimicrobial activity was assayed by broth microdilution techniques on bacteria and yeasts. The antioxidant activity was determined by DPPH radical scavenging method.</p> <p>Results</p> <p>Four known compounds [(5<it>S</it>,6<it>R</it>,8a<it>R</it>)-5-(carboxymethyl)-3,4,4a,5,6,7,8,8a-octahydro-5,6,8a-trimethylnaphthalenecarboxylic acid (<b>1</b>), methyl 3,4,5-trihydroxybenzoate (<b>2</b>), benzene-1,2,3-triol (<b>3</b>) and 2,3-dihydroxypropyltriacontanoate (<b>4</b>)] were isolated. Compared to the methanol extract, fractionation increased the antibacterial activities of the <it>n</it>-hexane and ethyl acetate fractions, while the antifungal activities increased in ethyl acetate, <it>n</it>-butanol and aqueous residue fractions. The isolated compounds were generally more active on bacteria (9.7 to 156.2 μg/ml) than yeasts (78.1 to 312.5 μg/ml). Apart from compound <b>1</b>, the three others displayed DPPH<sup>· </sup>scavenging activity (RSa), with RSa<sub>50 </sub>values of 1.45 and 1.60 μg/ml.</p> <p>Conclusion</p> <p>The results obtained from this study support the ethnomedicinal use of <it>E. abyssinica </it>in the treatment of gastrointestinal infections and the isolated compounds could be useful in the standardisation of antimicrobial phytomedicine from this plant.</p> |
| first_indexed | 2024-04-13T06:48:41Z |
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| institution | Directory Open Access Journal |
| issn | 1472-6882 |
| language | English |
| last_indexed | 2024-04-13T06:48:41Z |
| publishDate | 2011-07-01 |
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| series | BMC Complementary and Alternative Medicine |
| spelling | doaj.art-c08318fb560e4053a9af7f8441e9e9522022-12-22T02:57:29ZengBMCBMC Complementary and Alternative Medicine1472-68822011-07-011115710.1186/1472-6882-11-57Antimicrobial and antioxidant properties of methanol extract, fractions and compounds from the stem bark of <it>Entada abyssinica </it>Stend ex A. SatabieGiacinti GeraldineVilarem GerardKuiate Jules-RogerWabo Hippolyte KLunga Paul KTeke Gerald NKikuchi HaruhisaOshima Yoshiteru<p>Abstract</p> <p>Background</p> <p>The aim of this study was to evaluate the antimicrobial and antioxidant activities of the methanol extract, fractions and isolated compounds from <it>Entada abyssinica </it>stem bark, plant used traditionally against gastrointestinal infections.</p> <p>Methods</p> <p>The methanol extract of <it>E. abyssinica </it>stem bark was pre-dissolved in a mixture of methanol and water, and then partitioned between <it>n</it>-hexane, ethyl acetate and <it>n</it>-butanol. The ethyl acetate portion was fractionated by column chromatography and the structures of isolated compounds elucidated by analysis of spectroscopic data and comparison with literature data. Antimicrobial activity was assayed by broth microdilution techniques on bacteria and yeasts. The antioxidant activity was determined by DPPH radical scavenging method.</p> <p>Results</p> <p>Four known compounds [(5<it>S</it>,6<it>R</it>,8a<it>R</it>)-5-(carboxymethyl)-3,4,4a,5,6,7,8,8a-octahydro-5,6,8a-trimethylnaphthalenecarboxylic acid (<b>1</b>), methyl 3,4,5-trihydroxybenzoate (<b>2</b>), benzene-1,2,3-triol (<b>3</b>) and 2,3-dihydroxypropyltriacontanoate (<b>4</b>)] were isolated. Compared to the methanol extract, fractionation increased the antibacterial activities of the <it>n</it>-hexane and ethyl acetate fractions, while the antifungal activities increased in ethyl acetate, <it>n</it>-butanol and aqueous residue fractions. The isolated compounds were generally more active on bacteria (9.7 to 156.2 μg/ml) than yeasts (78.1 to 312.5 μg/ml). Apart from compound <b>1</b>, the three others displayed DPPH<sup>· </sup>scavenging activity (RSa), with RSa<sub>50 </sub>values of 1.45 and 1.60 μg/ml.</p> <p>Conclusion</p> <p>The results obtained from this study support the ethnomedicinal use of <it>E. abyssinica </it>in the treatment of gastrointestinal infections and the isolated compounds could be useful in the standardisation of antimicrobial phytomedicine from this plant.</p>http://www.biomedcentral.com/1472-6882/11/57 |
| spellingShingle | Giacinti Geraldine Vilarem Gerard Kuiate Jules-Roger Wabo Hippolyte K Lunga Paul K Teke Gerald N Kikuchi Haruhisa Oshima Yoshiteru Antimicrobial and antioxidant properties of methanol extract, fractions and compounds from the stem bark of <it>Entada abyssinica </it>Stend ex A. Satabie BMC Complementary and Alternative Medicine |
| title | Antimicrobial and antioxidant properties of methanol extract, fractions and compounds from the stem bark of <it>Entada abyssinica </it>Stend ex A. Satabie |
| title_full | Antimicrobial and antioxidant properties of methanol extract, fractions and compounds from the stem bark of <it>Entada abyssinica </it>Stend ex A. Satabie |
| title_fullStr | Antimicrobial and antioxidant properties of methanol extract, fractions and compounds from the stem bark of <it>Entada abyssinica </it>Stend ex A. Satabie |
| title_full_unstemmed | Antimicrobial and antioxidant properties of methanol extract, fractions and compounds from the stem bark of <it>Entada abyssinica </it>Stend ex A. Satabie |
| title_short | Antimicrobial and antioxidant properties of methanol extract, fractions and compounds from the stem bark of <it>Entada abyssinica </it>Stend ex A. Satabie |
| title_sort | antimicrobial and antioxidant properties of methanol extract fractions and compounds from the stem bark of it entada abyssinica it stend ex a satabie |
| url | http://www.biomedcentral.com/1472-6882/11/57 |
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