Comparative Analysis of Genetic Determinants Encoding Cadmium, Arsenic, and Benzalkonium Chloride Resistance in Listeria monocytogenes of Human, Food, and Environmental Origin
Environmental adaptation of Listeria monocytogenes is a complex process involving various mechanisms that can contribute to their survival in the environment, further spreading throughout the food chain and the development of listeriosis. The aim of this study was to analyze whole-genome sequencing...
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Frontiers Media S.A.
2021-01-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2020.599882/full |
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| author | Tereza Gelbicova Martina Florianova Lucie Hluchanova Lucie Hluchanova Alžběta Kalova Kristýna Korena Nicol Strakova Renáta Karpiskova Renáta Karpiskova |
| author_facet | Tereza Gelbicova Martina Florianova Lucie Hluchanova Lucie Hluchanova Alžběta Kalova Kristýna Korena Nicol Strakova Renáta Karpiskova Renáta Karpiskova |
| author_sort | Tereza Gelbicova |
| collection | DOAJ |
| description | Environmental adaptation of Listeria monocytogenes is a complex process involving various mechanisms that can contribute to their survival in the environment, further spreading throughout the food chain and the development of listeriosis. The aim of this study was to analyze whole-genome sequencing data in a set of 270 strains of L. monocytogenes derived from human listeriosis cases and food and environmental sources in order to compare the prevalence and type of genetic determinants encoding cadmium, arsenic, and benzalkonium chloride resistance. Most of the detected genes of cadmium (27.8%), arsenic (15.6%), and benzalkonium chloride (7.0%) resistance were located on mobile genetic elements, even in phylogenetically distant lineages I and II, which indicates the possibility of their horizontal spread. Although no differences were found in the prevalence of these genes between human and food strains, they have been detected sporadically in strains from the environment. Regarding cadmium resistance genes, cadA1C1_Tn5422 predominated, especially in clonal complexes (CCs) 121, 8, and 3 strains. At the same time, qacH_Tn6188-encoding benzalkonium chloride resistance was most frequently detected in the genome of CC121 strains. Genes encoding arsenic resistance were detected mainly in strains CC2 (located on the chromosomal island LGI2) and CC9 (carried on Tn554). The results indicated a relationship between the spread of genes encoding resistance to cadmium, arsenic, and benzalkonium chloride in certain serotypes and CCs and showed the need for a more extensive study of L. monocytogenes strains to better understand their ability to adapt to the food production environment. |
| first_indexed | 2024-12-14T00:53:18Z |
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| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-14T00:53:18Z |
| publishDate | 2021-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-c0855c30c9c04f00a0f67154e44955222022-12-21T23:23:44ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2021-01-011110.3389/fmicb.2020.599882599882Comparative Analysis of Genetic Determinants Encoding Cadmium, Arsenic, and Benzalkonium Chloride Resistance in Listeria monocytogenes of Human, Food, and Environmental OriginTereza Gelbicova0Martina Florianova1Lucie Hluchanova2Lucie Hluchanova3Alžběta Kalova4Kristýna Korena5Nicol Strakova6Renáta Karpiskova7Renáta Karpiskova8Department of Microbiology and Antibiotic Resistance, Veterinary Research Institute, Brno, CzechiaDepartment of Microbiology and Antibiotic Resistance, Veterinary Research Institute, Brno, CzechiaDepartment of Microbiology and Antibiotic Resistance, Veterinary Research Institute, Brno, CzechiaFaculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences Brno, Brno, CzechiaDepartment of Microbiology and Antibiotic Resistance, Veterinary Research Institute, Brno, CzechiaDepartment of Microbiology and Antibiotic Resistance, Veterinary Research Institute, Brno, CzechiaDepartment of Microbiology and Antibiotic Resistance, Veterinary Research Institute, Brno, CzechiaDepartment of Microbiology and Antibiotic Resistance, Veterinary Research Institute, Brno, CzechiaFaculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences Brno, Brno, CzechiaEnvironmental adaptation of Listeria monocytogenes is a complex process involving various mechanisms that can contribute to their survival in the environment, further spreading throughout the food chain and the development of listeriosis. The aim of this study was to analyze whole-genome sequencing data in a set of 270 strains of L. monocytogenes derived from human listeriosis cases and food and environmental sources in order to compare the prevalence and type of genetic determinants encoding cadmium, arsenic, and benzalkonium chloride resistance. Most of the detected genes of cadmium (27.8%), arsenic (15.6%), and benzalkonium chloride (7.0%) resistance were located on mobile genetic elements, even in phylogenetically distant lineages I and II, which indicates the possibility of their horizontal spread. Although no differences were found in the prevalence of these genes between human and food strains, they have been detected sporadically in strains from the environment. Regarding cadmium resistance genes, cadA1C1_Tn5422 predominated, especially in clonal complexes (CCs) 121, 8, and 3 strains. At the same time, qacH_Tn6188-encoding benzalkonium chloride resistance was most frequently detected in the genome of CC121 strains. Genes encoding arsenic resistance were detected mainly in strains CC2 (located on the chromosomal island LGI2) and CC9 (carried on Tn554). The results indicated a relationship between the spread of genes encoding resistance to cadmium, arsenic, and benzalkonium chloride in certain serotypes and CCs and showed the need for a more extensive study of L. monocytogenes strains to better understand their ability to adapt to the food production environment.https://www.frontiersin.org/articles/10.3389/fmicb.2020.599882/fullListeriacomparative genomicscadmiumarsenicbenzalkonium chlorideresistance |
| spellingShingle | Tereza Gelbicova Martina Florianova Lucie Hluchanova Lucie Hluchanova Alžběta Kalova Kristýna Korena Nicol Strakova Renáta Karpiskova Renáta Karpiskova Comparative Analysis of Genetic Determinants Encoding Cadmium, Arsenic, and Benzalkonium Chloride Resistance in Listeria monocytogenes of Human, Food, and Environmental Origin Frontiers in Microbiology Listeria comparative genomics cadmium arsenic benzalkonium chloride resistance |
| title | Comparative Analysis of Genetic Determinants Encoding Cadmium, Arsenic, and Benzalkonium Chloride Resistance in Listeria monocytogenes of Human, Food, and Environmental Origin |
| title_full | Comparative Analysis of Genetic Determinants Encoding Cadmium, Arsenic, and Benzalkonium Chloride Resistance in Listeria monocytogenes of Human, Food, and Environmental Origin |
| title_fullStr | Comparative Analysis of Genetic Determinants Encoding Cadmium, Arsenic, and Benzalkonium Chloride Resistance in Listeria monocytogenes of Human, Food, and Environmental Origin |
| title_full_unstemmed | Comparative Analysis of Genetic Determinants Encoding Cadmium, Arsenic, and Benzalkonium Chloride Resistance in Listeria monocytogenes of Human, Food, and Environmental Origin |
| title_short | Comparative Analysis of Genetic Determinants Encoding Cadmium, Arsenic, and Benzalkonium Chloride Resistance in Listeria monocytogenes of Human, Food, and Environmental Origin |
| title_sort | comparative analysis of genetic determinants encoding cadmium arsenic and benzalkonium chloride resistance in listeria monocytogenes of human food and environmental origin |
| topic | Listeria comparative genomics cadmium arsenic benzalkonium chloride resistance |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2020.599882/full |
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