Profiling bacterial communities by MinION sequencing of ribosomal operons

Profiling bacterial communities by MinION sequencing of ribosomal operons

Abstract Background An approach utilizing the long-read capability of the Oxford Nanopore MinION to rapidly sequence bacterial ribosomal operons of complex natural communities was developed. Microbial fingerprinting employs domain-specific forward primers (16S rRNA subunit), reverse primers (23S rRN...

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Bibliographic Details
Main Authors: Lee J. Kerkhof, Kevin P. Dillon, Max M. Häggblom, Lora R. McGuinness
Format: Article
Language:English
Published: BMC 2017-09-01
Series:Microbiome
Subjects:
Microbiota
Ribosomal operon
MinION
Species/strain-level resolution
Online Access:http://link.springer.com/article/10.1186/s40168-017-0336-9

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