GC-MS validated phytochemical up-leveling with in vitro-raised Sansevieria trifasciata [Prain]: The Mother in Law’s tongue gets more antibacterial
Antimicrobial resistance (AMR) poses a critical global health challenge, jeopardizing the efficacy of infectious disease treatments and necessitating innovative solutions. The exploration of plants, renowned for their wealth of bioactive compounds, as sustainable sources of antimicrobials is gaining...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-09-01
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| Series: | Current Plant Biology |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2214662823000373 |
| _version_ | 1797390504960196608 |
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| author | Jaspreet Kaur Sanjoy K. Dhar Arjun Chauhan Sweta Yadav Gaurav Mudgal Asyakina Lyudmila Victor Atuchin Gholamreza Abdi |
| author_facet | Jaspreet Kaur Sanjoy K. Dhar Arjun Chauhan Sweta Yadav Gaurav Mudgal Asyakina Lyudmila Victor Atuchin Gholamreza Abdi |
| author_sort | Jaspreet Kaur |
| collection | DOAJ |
| description | Antimicrobial resistance (AMR) poses a critical global health challenge, jeopardizing the efficacy of infectious disease treatments and necessitating innovative solutions. The exploration of plants, renowned for their wealth of bioactive compounds, as sustainable sources of antimicrobials is gaining momentum. Sansevieria, a succulent plant genus, boasts ornamental, medicinal, and pollution-remediating attributes owing to its phytochemical richness. Notably, specific species exhibit inherent antimicrobial properties, positioning them as promising reservoirs of alternative antimicrobial agents. Efficient cultivation methods are imperative for the economically viable production of Sansevierias with potential medicinal benefits. In addressing this need, in vitro micropropagation emerges as a strategic solution, enabling mass multiplication and germplasm conservation. Leveraging our previous success in achieving efficient in vitro regeneration of Sansevieria trifasciata through elevated temperatures and auxin supplementation, we present a novel PTC-assisted antimicrobial elicitation platform. This innovative approach resulted in significantly augmented biomass production, heightened concentrations of potent phytochemicals, and increased antibacterial activity in tissue extracts compared to their field-grown counterparts. Gas chromatography-mass spectrometry (GCMS) profiling confirmed the upregulation of phytochemicals known for their antibacterial efficacy. Notably, Citronellol, 7,8-Epoxylanostan-11-ol, 3-acetoxy, and several new compounds were markedly elevated in PTC-raised Sansevieria trifasciata. Our study furnishes compelling evidence for the efficacy of in vitro techniques in enhancing the growth and phytochemical profiles of Sansevieria plants. This opens new avenues for their commercial production, particularly in addressing the challenges posed by antimicrobial resistance. Importantly, this study represents the first exploration of phytochemical bioprospecting in the Sansevieria genus through in vitro tissue culture endeavors. |
| first_indexed | 2024-03-08T23:11:50Z |
| format | Article |
| id | doaj.art-c088dc225f464fd58ba3cafe4aa6d6b1 |
| institution | Directory Open Access Journal |
| issn | 2214-6628 |
| language | English |
| last_indexed | 2024-03-08T23:11:50Z |
| publishDate | 2023-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Current Plant Biology |
| spelling | doaj.art-c088dc225f464fd58ba3cafe4aa6d6b12023-12-15T07:24:28ZengElsevierCurrent Plant Biology2214-66282023-09-0135100308GC-MS validated phytochemical up-leveling with in vitro-raised Sansevieria trifasciata [Prain]: The Mother in Law’s tongue gets more antibacterialJaspreet Kaur0Sanjoy K. Dhar1Arjun Chauhan2Sweta Yadav3Gaurav Mudgal4Asyakina Lyudmila5Victor Atuchin6Gholamreza Abdi7University Institute of Biotechnology, Chandigarh University, Mohali 140413, Punjab, IndiaUniversity Institute of Biotechnology, Chandigarh University, Mohali 140413, Punjab, IndiaDepartment of Biotechnology, Institute of Applied Sciences & Humanities, GLA University, Mathura 281406, Uttar Pradesh, IndiaUniversity Institute of Biotechnology, Chandigarh University, Mohali 140413, Punjab, IndiaUniversity Institute of Biotechnology, Chandigarh University, Mohali 140413, Punjab, India; Corresponding authors.Laboratory for Phytoremediation of Technogenically Disturbed Ecosystems, Kemerovo State University, Krasnaya Street, 6, 650043 Kemerovo, RussiaLaboratory of Optical Materials and Structures, Institute of Semiconductor Physics, SB RAS, Novosibirsk 630090, Russia; Research and Development Department, Kemerovo State University, Kemerovo 650000, Russia; Department of Industrial Machinery Design, Novosibirsk State Technical University, Novosibirsk 630073, Russia; R&DCenter “Advanced Electronic Technologies”, Tomsk State University, Tomsk 634034, RussiaDepartment of Biotechnology, Persian Gulf Research Institute, Persian Gulf University, Bushehr 75169, Iran; Corresponding authors.Antimicrobial resistance (AMR) poses a critical global health challenge, jeopardizing the efficacy of infectious disease treatments and necessitating innovative solutions. The exploration of plants, renowned for their wealth of bioactive compounds, as sustainable sources of antimicrobials is gaining momentum. Sansevieria, a succulent plant genus, boasts ornamental, medicinal, and pollution-remediating attributes owing to its phytochemical richness. Notably, specific species exhibit inherent antimicrobial properties, positioning them as promising reservoirs of alternative antimicrobial agents. Efficient cultivation methods are imperative for the economically viable production of Sansevierias with potential medicinal benefits. In addressing this need, in vitro micropropagation emerges as a strategic solution, enabling mass multiplication and germplasm conservation. Leveraging our previous success in achieving efficient in vitro regeneration of Sansevieria trifasciata through elevated temperatures and auxin supplementation, we present a novel PTC-assisted antimicrobial elicitation platform. This innovative approach resulted in significantly augmented biomass production, heightened concentrations of potent phytochemicals, and increased antibacterial activity in tissue extracts compared to their field-grown counterparts. Gas chromatography-mass spectrometry (GCMS) profiling confirmed the upregulation of phytochemicals known for their antibacterial efficacy. Notably, Citronellol, 7,8-Epoxylanostan-11-ol, 3-acetoxy, and several new compounds were markedly elevated in PTC-raised Sansevieria trifasciata. Our study furnishes compelling evidence for the efficacy of in vitro techniques in enhancing the growth and phytochemical profiles of Sansevieria plants. This opens new avenues for their commercial production, particularly in addressing the challenges posed by antimicrobial resistance. Importantly, this study represents the first exploration of phytochemical bioprospecting in the Sansevieria genus through in vitro tissue culture endeavors.http://www.sciencedirect.com/science/article/pii/S2214662823000373Antimicrobial resistancePhytochemicalTissue cultureBioprospectingPhysiological profileCitronellol |
| spellingShingle | Jaspreet Kaur Sanjoy K. Dhar Arjun Chauhan Sweta Yadav Gaurav Mudgal Asyakina Lyudmila Victor Atuchin Gholamreza Abdi GC-MS validated phytochemical up-leveling with in vitro-raised Sansevieria trifasciata [Prain]: The Mother in Law’s tongue gets more antibacterial Current Plant Biology Antimicrobial resistance Phytochemical Tissue culture Bioprospecting Physiological profile Citronellol |
| title | GC-MS validated phytochemical up-leveling with in vitro-raised Sansevieria trifasciata [Prain]: The Mother in Law’s tongue gets more antibacterial |
| title_full | GC-MS validated phytochemical up-leveling with in vitro-raised Sansevieria trifasciata [Prain]: The Mother in Law’s tongue gets more antibacterial |
| title_fullStr | GC-MS validated phytochemical up-leveling with in vitro-raised Sansevieria trifasciata [Prain]: The Mother in Law’s tongue gets more antibacterial |
| title_full_unstemmed | GC-MS validated phytochemical up-leveling with in vitro-raised Sansevieria trifasciata [Prain]: The Mother in Law’s tongue gets more antibacterial |
| title_short | GC-MS validated phytochemical up-leveling with in vitro-raised Sansevieria trifasciata [Prain]: The Mother in Law’s tongue gets more antibacterial |
| title_sort | gc ms validated phytochemical up leveling with in vitro raised sansevieria trifasciata prain the mother in law s tongue gets more antibacterial |
| topic | Antimicrobial resistance Phytochemical Tissue culture Bioprospecting Physiological profile Citronellol |
| url | http://www.sciencedirect.com/science/article/pii/S2214662823000373 |
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