Encapsulation of Cochleates Derived from <i>Salmonella</i> Infantis with Biopolymers to Develop a Potential Oral Poultry Vaccine
The aim of this study was to develop and characterize <i>Salmonella</i><i>enterica</i> serovar Infantis (<i>S.</i> Infantis) cochleates protected by encapsulation technology as a potential vaccine and to determine its safety in pullets. Cochleates were encapsulate...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-10-01
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| Series: | Polymers |
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| Online Access: | https://www.mdpi.com/2073-4360/13/19/3426 |
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| author | Constanza Avendaño Sonia Vidal María Gabriela Villamizar-Sarmiento Miguel Guzmán Héctor Hidalgo Lisette Lapierre Carolina Valenzuela Leonardo Sáenz |
| author_facet | Constanza Avendaño Sonia Vidal María Gabriela Villamizar-Sarmiento Miguel Guzmán Héctor Hidalgo Lisette Lapierre Carolina Valenzuela Leonardo Sáenz |
| author_sort | Constanza Avendaño |
| collection | DOAJ |
| description | The aim of this study was to develop and characterize <i>Salmonella</i><i>enterica</i> serovar Infantis (<i>S.</i> Infantis) cochleates protected by encapsulation technology as a potential vaccine and to determine its safety in pullets. Cochleates were encapsulated by two technologies, spray drying and ionotropic gelation at different concentrations (0–15% v/v), and were characterized by physicochemical properties, protein content and Fourier Transform Infrared Spectroscopy (FTIR). The cochleates were white liquid suspensions with tubular shapes and a protein content of 1.0–2.1 mg/mL. After encapsulation by spray drying, microparticles ranged in size from 10.4–16.9 µm, were spherical in shape, and the protein content was 0.7–1.8 mg/g. After encapsulation by ionotropic gelation, beads ranged in size from 1620–1950 µm and were spherical in shape with a protein content of 1.0–2.5 mg/g. FTIR analysis indicated that both encapsulation processes were efficient. The cochleates encapsulated by ionotropic gelation were then tested for safety in pullets. No ill effect on the health of animals was observed upon physical or postmortem examination. In conclusion, this study was the first step in developing a potential oral <i>S.</i> Infantis vaccine safe for poultry using a novel cochleate encapsulation technology. Future studies are needed to determine the effectiveness of the vaccine. |
| first_indexed | 2024-03-10T06:53:23Z |
| format | Article |
| id | doaj.art-c08a4c11cc4d44dbb210e68213cc0092 |
| institution | Directory Open Access Journal |
| issn | 2073-4360 |
| language | English |
| last_indexed | 2024-03-10T06:53:23Z |
| publishDate | 2021-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Polymers |
| spelling | doaj.art-c08a4c11cc4d44dbb210e68213cc00922023-11-22T16:40:29ZengMDPI AGPolymers2073-43602021-10-011319342610.3390/polym13193426Encapsulation of Cochleates Derived from <i>Salmonella</i> Infantis with Biopolymers to Develop a Potential Oral Poultry VaccineConstanza Avendaño0Sonia Vidal1María Gabriela Villamizar-Sarmiento2Miguel Guzmán3Héctor Hidalgo4Lisette Lapierre5Carolina Valenzuela6Leonardo Sáenz7Faculty of Veterinary Sciences, University of Chile, Santiago 8820808, ChileFaculty of Veterinary Sciences, University of Chile, Santiago 8820808, ChileFaculty of Veterinary Sciences, University of Chile, Santiago 8820808, ChileFaculty of Veterinary Sciences, University of Chile, Santiago 8820808, ChileFaculty of Veterinary Sciences, University of Chile, Santiago 8820808, ChileFaculty of Veterinary Sciences, University of Chile, Santiago 8820808, ChileFaculty of Veterinary Sciences, University of Chile, Santiago 8820808, ChileFaculty of Veterinary Sciences, University of Chile, Santiago 8820808, ChileThe aim of this study was to develop and characterize <i>Salmonella</i><i>enterica</i> serovar Infantis (<i>S.</i> Infantis) cochleates protected by encapsulation technology as a potential vaccine and to determine its safety in pullets. Cochleates were encapsulated by two technologies, spray drying and ionotropic gelation at different concentrations (0–15% v/v), and were characterized by physicochemical properties, protein content and Fourier Transform Infrared Spectroscopy (FTIR). The cochleates were white liquid suspensions with tubular shapes and a protein content of 1.0–2.1 mg/mL. After encapsulation by spray drying, microparticles ranged in size from 10.4–16.9 µm, were spherical in shape, and the protein content was 0.7–1.8 mg/g. After encapsulation by ionotropic gelation, beads ranged in size from 1620–1950 µm and were spherical in shape with a protein content of 1.0–2.5 mg/g. FTIR analysis indicated that both encapsulation processes were efficient. The cochleates encapsulated by ionotropic gelation were then tested for safety in pullets. No ill effect on the health of animals was observed upon physical or postmortem examination. In conclusion, this study was the first step in developing a potential oral <i>S.</i> Infantis vaccine safe for poultry using a novel cochleate encapsulation technology. Future studies are needed to determine the effectiveness of the vaccine.https://www.mdpi.com/2073-4360/13/19/3426cochleateencapsulationpoultry<i>Salmonella</i> Infantisvaccine |
| spellingShingle | Constanza Avendaño Sonia Vidal María Gabriela Villamizar-Sarmiento Miguel Guzmán Héctor Hidalgo Lisette Lapierre Carolina Valenzuela Leonardo Sáenz Encapsulation of Cochleates Derived from <i>Salmonella</i> Infantis with Biopolymers to Develop a Potential Oral Poultry Vaccine Polymers cochleate encapsulation poultry <i>Salmonella</i> Infantis vaccine |
| title | Encapsulation of Cochleates Derived from <i>Salmonella</i> Infantis with Biopolymers to Develop a Potential Oral Poultry Vaccine |
| title_full | Encapsulation of Cochleates Derived from <i>Salmonella</i> Infantis with Biopolymers to Develop a Potential Oral Poultry Vaccine |
| title_fullStr | Encapsulation of Cochleates Derived from <i>Salmonella</i> Infantis with Biopolymers to Develop a Potential Oral Poultry Vaccine |
| title_full_unstemmed | Encapsulation of Cochleates Derived from <i>Salmonella</i> Infantis with Biopolymers to Develop a Potential Oral Poultry Vaccine |
| title_short | Encapsulation of Cochleates Derived from <i>Salmonella</i> Infantis with Biopolymers to Develop a Potential Oral Poultry Vaccine |
| title_sort | encapsulation of cochleates derived from i salmonella i infantis with biopolymers to develop a potential oral poultry vaccine |
| topic | cochleate encapsulation poultry <i>Salmonella</i> Infantis vaccine |
| url | https://www.mdpi.com/2073-4360/13/19/3426 |
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