Leukocyte Recruitment to Peritoneal Cavity of Rats Following Formalin Injection: Role of Tachykinin Receptors
The aim of this work was to verify whether formalin would induce leukocyte recruitment following intraperitoneal (i.p.) injection in rats. Formalin (1.25 – 2.5%) induced cell recruitment, which was concentration- and time-dependent (0 – 24 h). Two peaks of leukocyte recruitment were observed. The fi...
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| Format: | Article |
| Language: | English |
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Elsevier
2004-01-01
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| Series: | Journal of Pharmacological Sciences |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1347861319324739 |
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| author | Júlia M.M. Santos Maria Aparecida K.F. Tatsuo Regina Maria M. Turchetti-Maia Marcela C.G. Lisboa Janetti N. de Francischi |
| author_facet | Júlia M.M. Santos Maria Aparecida K.F. Tatsuo Regina Maria M. Turchetti-Maia Marcela C.G. Lisboa Janetti N. de Francischi |
| author_sort | Júlia M.M. Santos |
| collection | DOAJ |
| description | The aim of this work was to verify whether formalin would induce leukocyte recruitment following intraperitoneal (i.p.) injection in rats. Formalin (1.25 – 2.5%) induced cell recruitment, which was concentration- and time-dependent (0 – 24 h). Two peaks of leukocyte recruitment were observed. The first peak (from 2 to 4 h) was characterized by a mixed polymorphonuclear and lymphocyte cell population (representing an increase of 100 – 220% and 55 – 60%, respectively), whereas the second peak was characterized by a marked increase in lymphocytes at 24 h (representing an increase of 230%). Pretreatment of animals with specific antagonists for neurokinin NK1, NK2, and NK3 receptors (SR140333, SR48968, and SR142801 compounds, respectively) reduced the early leukocyte increase (representing a significant reduction of 65%, 51%, and 46%, respectively), whereas only the treatment with NK2-specific antagonist reduced the late cell increase induced by formalin injection (amounting to a significant reduction of 48%). These results suggested that substance P, neurokinin A, and neurokinin B release accounted for formalin-induced cell migratory activity. The anti-inflammatory drug dexamethasone also reduced cell recruitment, which was mainly related to a reduction in 79% of the neutrophils at 4 h following 1.25% formalin injection, suggesting also a release of lipid mediators (eicosanoids and/or platelet-activating factor) and/or cytokines/chemokines by the formalin injection. Keywords:: formalin, leukocyte, cell migration, tachykinin receptor, neurokinin |
| first_indexed | 2024-04-13T15:23:39Z |
| format | Article |
| id | doaj.art-c08e12f1ee78471a8e399074ead444c1 |
| institution | Directory Open Access Journal |
| issn | 1347-8613 |
| language | English |
| last_indexed | 2024-04-13T15:23:39Z |
| publishDate | 2004-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Pharmacological Sciences |
| spelling | doaj.art-c08e12f1ee78471a8e399074ead444c12022-12-22T02:41:35ZengElsevierJournal of Pharmacological Sciences1347-86132004-01-01944384392Leukocyte Recruitment to Peritoneal Cavity of Rats Following Formalin Injection: Role of Tachykinin ReceptorsJúlia M.M. Santos0Maria Aparecida K.F. Tatsuo1Regina Maria M. Turchetti-Maia2Marcela C.G. Lisboa3Janetti N. de Francischi4Department of Pharmacology, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, BrazilDepartment of Pharmacology, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, BrazilDepartment of Pharmacology, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, BrazilDepartment of Pharmacology, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, BrazilDepartment of Pharmacology, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, Brazil; Corresponding author. FAX: +55-31-3499-2695 E-mail: janettif@mono.icb.ufmg.brThe aim of this work was to verify whether formalin would induce leukocyte recruitment following intraperitoneal (i.p.) injection in rats. Formalin (1.25 – 2.5%) induced cell recruitment, which was concentration- and time-dependent (0 – 24 h). Two peaks of leukocyte recruitment were observed. The first peak (from 2 to 4 h) was characterized by a mixed polymorphonuclear and lymphocyte cell population (representing an increase of 100 – 220% and 55 – 60%, respectively), whereas the second peak was characterized by a marked increase in lymphocytes at 24 h (representing an increase of 230%). Pretreatment of animals with specific antagonists for neurokinin NK1, NK2, and NK3 receptors (SR140333, SR48968, and SR142801 compounds, respectively) reduced the early leukocyte increase (representing a significant reduction of 65%, 51%, and 46%, respectively), whereas only the treatment with NK2-specific antagonist reduced the late cell increase induced by formalin injection (amounting to a significant reduction of 48%). These results suggested that substance P, neurokinin A, and neurokinin B release accounted for formalin-induced cell migratory activity. The anti-inflammatory drug dexamethasone also reduced cell recruitment, which was mainly related to a reduction in 79% of the neutrophils at 4 h following 1.25% formalin injection, suggesting also a release of lipid mediators (eicosanoids and/or platelet-activating factor) and/or cytokines/chemokines by the formalin injection. Keywords:: formalin, leukocyte, cell migration, tachykinin receptor, neurokininhttp://www.sciencedirect.com/science/article/pii/S1347861319324739 |
| spellingShingle | Júlia M.M. Santos Maria Aparecida K.F. Tatsuo Regina Maria M. Turchetti-Maia Marcela C.G. Lisboa Janetti N. de Francischi Leukocyte Recruitment to Peritoneal Cavity of Rats Following Formalin Injection: Role of Tachykinin Receptors Journal of Pharmacological Sciences |
| title | Leukocyte Recruitment to Peritoneal Cavity of Rats Following Formalin Injection: Role of Tachykinin Receptors |
| title_full | Leukocyte Recruitment to Peritoneal Cavity of Rats Following Formalin Injection: Role of Tachykinin Receptors |
| title_fullStr | Leukocyte Recruitment to Peritoneal Cavity of Rats Following Formalin Injection: Role of Tachykinin Receptors |
| title_full_unstemmed | Leukocyte Recruitment to Peritoneal Cavity of Rats Following Formalin Injection: Role of Tachykinin Receptors |
| title_short | Leukocyte Recruitment to Peritoneal Cavity of Rats Following Formalin Injection: Role of Tachykinin Receptors |
| title_sort | leukocyte recruitment to peritoneal cavity of rats following formalin injection role of tachykinin receptors |
| url | http://www.sciencedirect.com/science/article/pii/S1347861319324739 |
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