Geographical identification of Larimichthys crocea based on analysis of elements and stable isotope ratios(基于元素及稳定同位素分析的大黄鱼产地区分技术)

为实现对4种大黄鱼(Larimichthys crocea)产地(分别来自福建宁德养殖、浙江温州养殖、浙江舟山养殖和舟山渔场自然海域捕捞)的区分,应用电感耦合等离子体质谱仪(inductively coupled plasma mass spectrometry,ICP-MS)及元素分析-同位素质谱仪对大黄鱼肌肉中19种矿物元素及δ13C、δ15N和C、N元素的质量分数进行了测定,基于质量分数差异并结合主成分分析(principal component analysis,PCA)、聚类分析(cluster analysis,CA)和Fisher判别分析对4种不同产地大黄鱼进行区分。单因素方差分...

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Main Authors: MEIGuangming(梅光明), YANGYingyue(杨盈悦), ZHANGYuru(张玉汝), ZHAOYuehan(赵月涵), ZHANGXiaojun(张小军), HUANGLiying(黄丽英), ZHENGBin(郑斌)
Format: Article
Language:zho
Published: Zhejiang University Press 2022-11-01
Series:Zhejiang Daxue xuebao. Lixue ban
Subjects:
Online Access:https://doi.org/10.3785/j.issn.1008-9497.2022.06.010
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author MEIGuangming(梅光明)
YANGYingyue(杨盈悦)
ZHANGYuru(张玉汝)
ZHAOYuehan(赵月涵)
ZHANGXiaojun(张小军)
HUANGLiying(黄丽英)
ZHENGBin(郑斌)
author_facet MEIGuangming(梅光明)
YANGYingyue(杨盈悦)
ZHANGYuru(张玉汝)
ZHAOYuehan(赵月涵)
ZHANGXiaojun(张小军)
HUANGLiying(黄丽英)
ZHENGBin(郑斌)
author_sort MEIGuangming(梅光明)
collection DOAJ
description 为实现对4种大黄鱼(Larimichthys crocea)产地(分别来自福建宁德养殖、浙江温州养殖、浙江舟山养殖和舟山渔场自然海域捕捞)的区分,应用电感耦合等离子体质谱仪(inductively coupled plasma mass spectrometry,ICP-MS)及元素分析-同位素质谱仪对大黄鱼肌肉中19种矿物元素及δ13C、δ15N和C、N元素的质量分数进行了测定,基于质量分数差异并结合主成分分析(principal component analysis,PCA)、聚类分析(cluster analysis,CA)和Fisher判别分析对4种不同产地大黄鱼进行区分。单因素方差分析(ANOVA)及事后多重比较(LSD和Tamhane T2检验)结果表明,12种元素(C、N、Mg、Al、K、Ti、Cr、Cu、Zn、Se、Sn、Ba)及δ13C、δ15N在不同产地大黄鱼中具有较明显的地域特征差异。PCA结果表明,前2个主成分(累计方差贡献率为64.1%)的得分散点图分成了4个相对集中的区域,4种不同产地大黄鱼彼此无重叠,能被有效区分。CA结果表明,4种不同产地大黄鱼中除温州养殖大黄鱼样本被分为两类,其他3种大黄鱼样本均各自聚为一类。Fisher判别分析结果显示,原始分类结果的判别准确率为100%,“留一法”交叉验证分类结果显示,有85.7%的舟山养殖大黄鱼样本被正确分类,14.3%的被误判为温州养殖大黄鱼,其余3种大黄鱼样本分类准确率均为100%。研究结果表明,利用元素及稳定同位素比值差异结合化学计量学分析手段,可有效实现大黄鱼的产地区分,为大黄鱼产地鉴别提供参考。
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spelling doaj.art-c08ed2aa9d2746bd8d97a2e55ef748cb2024-03-29T01:58:40ZzhoZhejiang University PressZhejiang Daxue xuebao. Lixue ban1008-94972022-11-0149671572510.3785/j.issn.1008-9497.2022.06.010Geographical identification of Larimichthys crocea based on analysis of elements and stable isotope ratios(基于元素及稳定同位素分析的大黄鱼产地区分技术)MEIGuangming(梅光明)0https://orcid.org/0000-0002-9267-8112YANGYingyue(杨盈悦)1ZHANGYuru(张玉汝)2ZHAOYuehan(赵月涵)3ZHANGXiaojun(张小军)4https://orcid.org/0000-0001-8807-5450HUANGLiying(黄丽英)5ZHENGBin(郑斌)6 1.Zhejiang Marine Fisheries Research Institute, Zhoushan 316021, Zhejiang Province, China( 1.浙江省海洋水产研究所,浙江 舟山 316021) 1.Zhejiang Marine Fisheries Research Institute, Zhoushan 316021, Zhejiang Province, China( 1.浙江省海洋水产研究所,浙江 舟山 316021) 4.Zhouhuan Environmental Engineering Design Co., LTD, Zhoushan 316000, Zhejiang Province, China( 4.浙江舟山环境工程设计有限公司,浙江 舟山 316000) 1.Zhejiang Marine Fisheries Research Institute, Zhoushan 316021, Zhejiang Province, China( 1.浙江省海洋水产研究所,浙江 舟山 316021) 1.Zhejiang Marine Fisheries Research Institute, Zhoushan 316021, Zhejiang Province, China( 1.浙江省海洋水产研究所,浙江 舟山 316021) 1.Zhejiang Marine Fisheries Research Institute, Zhoushan 316021, Zhejiang Province, China( 1.浙江省海洋水产研究所,浙江 舟山 316021) 3.College of Food Science and Pharmacy, Zhejiang Ocean University, Zhoushan 316022, Zhejiang Province, China( 3.浙江海洋大学 食品与医药学院,浙江 舟山 316022)为实现对4种大黄鱼(Larimichthys crocea)产地(分别来自福建宁德养殖、浙江温州养殖、浙江舟山养殖和舟山渔场自然海域捕捞)的区分,应用电感耦合等离子体质谱仪(inductively coupled plasma mass spectrometry,ICP-MS)及元素分析-同位素质谱仪对大黄鱼肌肉中19种矿物元素及δ13C、δ15N和C、N元素的质量分数进行了测定,基于质量分数差异并结合主成分分析(principal component analysis,PCA)、聚类分析(cluster analysis,CA)和Fisher判别分析对4种不同产地大黄鱼进行区分。单因素方差分析(ANOVA)及事后多重比较(LSD和Tamhane T2检验)结果表明,12种元素(C、N、Mg、Al、K、Ti、Cr、Cu、Zn、Se、Sn、Ba)及δ13C、δ15N在不同产地大黄鱼中具有较明显的地域特征差异。PCA结果表明,前2个主成分(累计方差贡献率为64.1%)的得分散点图分成了4个相对集中的区域,4种不同产地大黄鱼彼此无重叠,能被有效区分。CA结果表明,4种不同产地大黄鱼中除温州养殖大黄鱼样本被分为两类,其他3种大黄鱼样本均各自聚为一类。Fisher判别分析结果显示,原始分类结果的判别准确率为100%,“留一法”交叉验证分类结果显示,有85.7%的舟山养殖大黄鱼样本被正确分类,14.3%的被误判为温州养殖大黄鱼,其余3种大黄鱼样本分类准确率均为100%。研究结果表明,利用元素及稳定同位素比值差异结合化学计量学分析手段,可有效实现大黄鱼的产地区分,为大黄鱼产地鉴别提供参考。https://doi.org/10.3785/j.issn.1008-9497.2022.06.010大黄鱼产地区分元素稳定同位素化学计量学
spellingShingle MEIGuangming(梅光明)
YANGYingyue(杨盈悦)
ZHANGYuru(张玉汝)
ZHAOYuehan(赵月涵)
ZHANGXiaojun(张小军)
HUANGLiying(黄丽英)
ZHENGBin(郑斌)
Geographical identification of Larimichthys crocea based on analysis of elements and stable isotope ratios(基于元素及稳定同位素分析的大黄鱼产地区分技术)
Zhejiang Daxue xuebao. Lixue ban
大黄鱼
产地区分
元素
稳定同位素
化学计量学
title Geographical identification of Larimichthys crocea based on analysis of elements and stable isotope ratios(基于元素及稳定同位素分析的大黄鱼产地区分技术)
title_full Geographical identification of Larimichthys crocea based on analysis of elements and stable isotope ratios(基于元素及稳定同位素分析的大黄鱼产地区分技术)
title_fullStr Geographical identification of Larimichthys crocea based on analysis of elements and stable isotope ratios(基于元素及稳定同位素分析的大黄鱼产地区分技术)
title_full_unstemmed Geographical identification of Larimichthys crocea based on analysis of elements and stable isotope ratios(基于元素及稳定同位素分析的大黄鱼产地区分技术)
title_short Geographical identification of Larimichthys crocea based on analysis of elements and stable isotope ratios(基于元素及稳定同位素分析的大黄鱼产地区分技术)
title_sort geographical identification of larimichthys crocea based on analysis of elements and stable isotope ratios 基于元素及稳定同位素分析的大黄鱼产地区分技术
topic 大黄鱼
产地区分
元素
稳定同位素
化学计量学
url https://doi.org/10.3785/j.issn.1008-9497.2022.06.010
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