Inducing the formation of a colloidal albumin carrier of curcumin
The administration and delivery of pharmaceuticals faces a variety of well-known obstacles that result in limited biocompatibility and bioavailability. Efforts to improve these properties have often employed serum albumin, primarily due to its inherent biocompatibility and its ability to enhance the...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-07-01
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| Series: | JCIS Open |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666934X22000095 |
| _version_ | 1818470713200214016 |
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| author | Konstantina Matskou Berke Kisaoglan Barbara Mavroidi Maria Pelecanou Maria Zoumpanioti Ilias Matis Aristotelis Xenakis |
| author_facet | Konstantina Matskou Berke Kisaoglan Barbara Mavroidi Maria Pelecanou Maria Zoumpanioti Ilias Matis Aristotelis Xenakis |
| author_sort | Konstantina Matskou |
| collection | DOAJ |
| description | The administration and delivery of pharmaceuticals faces a variety of well-known obstacles that result in limited biocompatibility and bioavailability. Efforts to improve these properties have often employed serum albumin, primarily due to its inherent biocompatibility and its ability to enhance the circulation times of pharmaceuticals. In this work, we have adapted a nanoparticle-formulation protocol, to produce a protein carrier of curcumin with bovine serum albumin. This was achieved by using a near-equimolar protein:curcumin ratio instead of the abundance of curcumin that would be normally used in a nanoparticle formulation. Photometric and quantitative analysis of this carrier showed an increased curcumin content in the produced aqueous solutions following the homogenization of bovine serum albumin (water) and curcumin (dichloromethane) phases. Albumin fluorescence studies indicated curcumin association near a tryptophan residue, without excluding the possibility of additional sites. Circular dichroism provided strong evidence of this association through the induced circular dichroism effect and showed that the secondary structure of bovine serum albumin was effectively maintained. Overall, this work presented a new means of facilitating the association of increased levels of curcumin with bovine serum albumin, which could potentially be used to generate additional non-covalent albumin carriers for pharmaceutical compounds. |
| first_indexed | 2024-04-13T21:41:01Z |
| format | Article |
| id | doaj.art-c091520aec2648f4b2ca1f60f9192531 |
| institution | Directory Open Access Journal |
| issn | 2666-934X |
| language | English |
| last_indexed | 2024-04-13T21:41:01Z |
| publishDate | 2022-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | JCIS Open |
| spelling | doaj.art-c091520aec2648f4b2ca1f60f91925312022-12-22T02:28:46ZengElsevierJCIS Open2666-934X2022-07-016100051Inducing the formation of a colloidal albumin carrier of curcuminKonstantina Matskou0Berke Kisaoglan1Barbara Mavroidi2Maria Pelecanou3Maria Zoumpanioti4Ilias Matis5Aristotelis Xenakis6Institute of Chemical Biology, National Hellenic Research Foundation, Athens, 11635, Greece; Department of Biology, National Kapodistrian University of Athens, Athens, 15701, GreeceInstitute of Chemical Biology, National Hellenic Research Foundation, Athens, 11635, GreeceInstitute of Biosciences and Applications, National Center for Scientific Research “Demokritos”, Athens, 15341, GreeceInstitute of Biosciences and Applications, National Center for Scientific Research “Demokritos”, Athens, 15341, GreeceInstitute of Chemical Biology, National Hellenic Research Foundation, Athens, 11635, GreeceInstitute of Chemical Biology, National Hellenic Research Foundation, Athens, 11635, Greece; Corresponding author.Institute of Chemical Biology, National Hellenic Research Foundation, Athens, 11635, Greece; Corresponding author. National Hellenic Research Foundation, Institute of Chemical Biology, 48, Vassileos Constantinou Av., 11635 Athens, Greece.The administration and delivery of pharmaceuticals faces a variety of well-known obstacles that result in limited biocompatibility and bioavailability. Efforts to improve these properties have often employed serum albumin, primarily due to its inherent biocompatibility and its ability to enhance the circulation times of pharmaceuticals. In this work, we have adapted a nanoparticle-formulation protocol, to produce a protein carrier of curcumin with bovine serum albumin. This was achieved by using a near-equimolar protein:curcumin ratio instead of the abundance of curcumin that would be normally used in a nanoparticle formulation. Photometric and quantitative analysis of this carrier showed an increased curcumin content in the produced aqueous solutions following the homogenization of bovine serum albumin (water) and curcumin (dichloromethane) phases. Albumin fluorescence studies indicated curcumin association near a tryptophan residue, without excluding the possibility of additional sites. Circular dichroism provided strong evidence of this association through the induced circular dichroism effect and showed that the secondary structure of bovine serum albumin was effectively maintained. Overall, this work presented a new means of facilitating the association of increased levels of curcumin with bovine serum albumin, which could potentially be used to generate additional non-covalent albumin carriers for pharmaceutical compounds.http://www.sciencedirect.com/science/article/pii/S2666934X22000095Bovine serum albuminHomogenizationNon-covalent associationEquimolar protein-binder |
| spellingShingle | Konstantina Matskou Berke Kisaoglan Barbara Mavroidi Maria Pelecanou Maria Zoumpanioti Ilias Matis Aristotelis Xenakis Inducing the formation of a colloidal albumin carrier of curcumin JCIS Open Bovine serum albumin Homogenization Non-covalent association Equimolar protein-binder |
| title | Inducing the formation of a colloidal albumin carrier of curcumin |
| title_full | Inducing the formation of a colloidal albumin carrier of curcumin |
| title_fullStr | Inducing the formation of a colloidal albumin carrier of curcumin |
| title_full_unstemmed | Inducing the formation of a colloidal albumin carrier of curcumin |
| title_short | Inducing the formation of a colloidal albumin carrier of curcumin |
| title_sort | inducing the formation of a colloidal albumin carrier of curcumin |
| topic | Bovine serum albumin Homogenization Non-covalent association Equimolar protein-binder |
| url | http://www.sciencedirect.com/science/article/pii/S2666934X22000095 |
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