The antioxidant, anti-angiogenic, and anticancer impact of chitosan-coated herniarin-graphene oxide nanoparticles (CHG-NPs)
Background: Herniarin, a simple coumarin found in chamomile leaf rosettes is known as the oxidative stress protector. In the current study, herniarin was captured into Graphene oxide nanoparticles and coated with chitosan poly-cationic polymer to be used as a novel bio-compatible nano-drug delivery...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-09-01
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| Series: | Heliyon |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S240584402307250X |
| _version_ | 1797669792420724736 |
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| author | Louay Mohammed Musa Jasim Masoud Homayouni Tabrizi Elham Darabi Muslem Mohammed Mosa Jaseem |
| author_facet | Louay Mohammed Musa Jasim Masoud Homayouni Tabrizi Elham Darabi Muslem Mohammed Mosa Jaseem |
| author_sort | Louay Mohammed Musa Jasim |
| collection | DOAJ |
| description | Background: Herniarin, a simple coumarin found in chamomile leaf rosettes is known as the oxidative stress protector. In the current study, herniarin was captured into Graphene oxide nanoparticles and coated with chitosan poly-cationic polymer to be used as a novel bio-compatible nano-drug delivery system and investigate its antioxidant, anti-angiogenic and anti-cancer impacts on human lung A549 cancer cells. Method: The Chitosan-coated Herniarin-Graphene oxide nanoparticles (CHG-NPs) were designed, produced, and characterized utilizing DLS, FESEM, FTIR, and Zeta-potential analysis. The CHG-NPs’ antioxidant activity was analyzed by conducting ABTS and DPPH antioxidant assays. The CHG-NPs’ anti-angiogenic activity was analyzed by CAM assay and verified by measuring VEGF and VEGFR gene expression levels following their increased treatment doses by applying Q-PCR technique. Finally, the CHG-NPs’ cytotoxicity was studied in the human lung A549 cancer cells. Result: The stable (+27.11 mV) 213.6-nm CHG-NPs significantly inhibited the ABTS/DPPH free radicals and exhibited antioxidant activity. The suppressed angiogenesis process in the CAM vessels was observed by detecting the decreased length/number of the vessels. Moreover, the down-regulated VEGF and VEGFR gene expression of the CAM blood vessels following the increased CHG-NPs treatment doses verified the nanoparticles’ anti-angiogenic potential. Finally, the CHG-NPs significantly exhibited a selective cytotoxic impact on human A549 cancer cells compared with the normal HFF cell line. Conclusion: The selective cytotoxicity, strong antioxidant activity, and significant anti-angiogenic property of the nano-scaled produced CHG-NPs make it an appropriate anticancer nano-drug delivery system. Therefore, the CHG-NPs have the potential to be used as a selective anti-lung cancer compound. |
| first_indexed | 2024-03-11T20:49:53Z |
| format | Article |
| id | doaj.art-c0931da7d5074031be6ff5bdd327f6fd |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-03-11T20:49:53Z |
| publishDate | 2023-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-c0931da7d5074031be6ff5bdd327f6fd2023-10-01T06:02:22ZengElsevierHeliyon2405-84402023-09-0199e20042The antioxidant, anti-angiogenic, and anticancer impact of chitosan-coated herniarin-graphene oxide nanoparticles (CHG-NPs)Louay Mohammed Musa Jasim0Masoud Homayouni Tabrizi1Elham Darabi2Muslem Mohammed Mosa Jaseem3Plasma Physics Research Center, Science and Research Branch, Islamic Azad University, Tehran, IranDepartment of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran; Corresponding author.Plasma Physics Research Center, Science and Research Branch, Islamic Azad University, Tehran, IranDepartment of Biology, Mashhad Branch, Islamic Azad University, Mashhad, IranBackground: Herniarin, a simple coumarin found in chamomile leaf rosettes is known as the oxidative stress protector. In the current study, herniarin was captured into Graphene oxide nanoparticles and coated with chitosan poly-cationic polymer to be used as a novel bio-compatible nano-drug delivery system and investigate its antioxidant, anti-angiogenic and anti-cancer impacts on human lung A549 cancer cells. Method: The Chitosan-coated Herniarin-Graphene oxide nanoparticles (CHG-NPs) were designed, produced, and characterized utilizing DLS, FESEM, FTIR, and Zeta-potential analysis. The CHG-NPs’ antioxidant activity was analyzed by conducting ABTS and DPPH antioxidant assays. The CHG-NPs’ anti-angiogenic activity was analyzed by CAM assay and verified by measuring VEGF and VEGFR gene expression levels following their increased treatment doses by applying Q-PCR technique. Finally, the CHG-NPs’ cytotoxicity was studied in the human lung A549 cancer cells. Result: The stable (+27.11 mV) 213.6-nm CHG-NPs significantly inhibited the ABTS/DPPH free radicals and exhibited antioxidant activity. The suppressed angiogenesis process in the CAM vessels was observed by detecting the decreased length/number of the vessels. Moreover, the down-regulated VEGF and VEGFR gene expression of the CAM blood vessels following the increased CHG-NPs treatment doses verified the nanoparticles’ anti-angiogenic potential. Finally, the CHG-NPs significantly exhibited a selective cytotoxic impact on human A549 cancer cells compared with the normal HFF cell line. Conclusion: The selective cytotoxicity, strong antioxidant activity, and significant anti-angiogenic property of the nano-scaled produced CHG-NPs make it an appropriate anticancer nano-drug delivery system. Therefore, the CHG-NPs have the potential to be used as a selective anti-lung cancer compound.http://www.sciencedirect.com/science/article/pii/S240584402307250XChitosan-coated herniarin-graphene oxide nanoparticles (CHG-NPs)Selective cytotoxicityAntioxidantAnti-angiogenicAnti-Lung cancer |
| spellingShingle | Louay Mohammed Musa Jasim Masoud Homayouni Tabrizi Elham Darabi Muslem Mohammed Mosa Jaseem The antioxidant, anti-angiogenic, and anticancer impact of chitosan-coated herniarin-graphene oxide nanoparticles (CHG-NPs) Heliyon Chitosan-coated herniarin-graphene oxide nanoparticles (CHG-NPs) Selective cytotoxicity Antioxidant Anti-angiogenic Anti-Lung cancer |
| title | The antioxidant, anti-angiogenic, and anticancer impact of chitosan-coated herniarin-graphene oxide nanoparticles (CHG-NPs) |
| title_full | The antioxidant, anti-angiogenic, and anticancer impact of chitosan-coated herniarin-graphene oxide nanoparticles (CHG-NPs) |
| title_fullStr | The antioxidant, anti-angiogenic, and anticancer impact of chitosan-coated herniarin-graphene oxide nanoparticles (CHG-NPs) |
| title_full_unstemmed | The antioxidant, anti-angiogenic, and anticancer impact of chitosan-coated herniarin-graphene oxide nanoparticles (CHG-NPs) |
| title_short | The antioxidant, anti-angiogenic, and anticancer impact of chitosan-coated herniarin-graphene oxide nanoparticles (CHG-NPs) |
| title_sort | antioxidant anti angiogenic and anticancer impact of chitosan coated herniarin graphene oxide nanoparticles chg nps |
| topic | Chitosan-coated herniarin-graphene oxide nanoparticles (CHG-NPs) Selective cytotoxicity Antioxidant Anti-angiogenic Anti-Lung cancer |
| url | http://www.sciencedirect.com/science/article/pii/S240584402307250X |
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