Enhanced tendon–bone healing with acidic fibroblast growth factor delivered in collagen in a rabbit anterior cruciate ligament reconstruction model

Abstract Background The objective of the present study was to investigate the effectiveness of acidic fibroblast growth factor delivered in collagen (aFGF/collagen) for promoting tendon–bone interface healing after anterior cruciate ligament (ACL) reconstruction in rabbits. Methods ACL reconstructio...

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Main Authors: Daifeng Lu, Chuandong Yang, Zhitao Zhang, Mochao Xiao
Format: Article
Language:English
Published: BMC 2018-11-01
Series:Journal of Orthopaedic Surgery and Research
Subjects:
Online Access:http://link.springer.com/article/10.1186/s13018-018-0984-x
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author Daifeng Lu
Chuandong Yang
Zhitao Zhang
Mochao Xiao
author_facet Daifeng Lu
Chuandong Yang
Zhitao Zhang
Mochao Xiao
author_sort Daifeng Lu
collection DOAJ
description Abstract Background The objective of the present study was to investigate the effectiveness of acidic fibroblast growth factor delivered in collagen (aFGF/collagen) for promoting tendon–bone interface healing after anterior cruciate ligament (ACL) reconstruction in rabbits. Methods ACL reconstructions were performed in the right hind limbs of New Zealand rabbits. Each left long digital extensor tendon was harvested as an autograft, and collagen incorporating different concentrations of aFGF or same amount of collagen alone was applied at the tendon–bone interface after ACL reconstruction. The control group underwent ACL reconstruction only. There were high and low aFGF/collagen groups, collagen alone group, and control group (n = 21 rabbits per group). Histological and biomechanical analyses were performed at 4, 8, and 12 weeks postoperatively to evaluate the effect of aFGF/collagen on tendon–bone interface healing. Results Results of biomechanical tests showed that at both 8 and 12 weeks postoperatively, the elastic modulus and stiffness in both the high and low aFGF/collagen treatment groups were significantly higher than those in the control group and collagen alone group, with that in the high aFGF/collagen concentration group being the highest. Histological analysis showed that at 8 weeks, tightly organized Sharpey-like fibers were observed in both aFGF/collagen groups with new bone growth into the tendon in the high concentration group. At 12 weeks postoperatively, a fibrocartilage transition zone was observed in the bone tunnels in both aFGF/collagen groups, especially in the high aFGF/collagen group. Conclusion Application of the aFGF/collagen composite could enhance early healing at the tendon–bone interface after ACL reconstruction, especially with the use of a high aFGF/collagen concentration.
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spelling doaj.art-c0958fb48beb46f7a77fd1a1095676e02022-12-22T04:19:51ZengBMCJournal of Orthopaedic Surgery and Research1749-799X2018-11-011311810.1186/s13018-018-0984-xEnhanced tendon–bone healing with acidic fibroblast growth factor delivered in collagen in a rabbit anterior cruciate ligament reconstruction modelDaifeng Lu0Chuandong Yang1Zhitao Zhang2Mochao Xiao3The Fourth Affiliated Hospital of Harbin Medical UniversityHeilongjiang Provincial Academy of Medical SciencesThe Fourth Affiliated Hospital of Harbin Medical UniversityThe Fourth Affiliated Hospital of Harbin Medical UniversityAbstract Background The objective of the present study was to investigate the effectiveness of acidic fibroblast growth factor delivered in collagen (aFGF/collagen) for promoting tendon–bone interface healing after anterior cruciate ligament (ACL) reconstruction in rabbits. Methods ACL reconstructions were performed in the right hind limbs of New Zealand rabbits. Each left long digital extensor tendon was harvested as an autograft, and collagen incorporating different concentrations of aFGF or same amount of collagen alone was applied at the tendon–bone interface after ACL reconstruction. The control group underwent ACL reconstruction only. There were high and low aFGF/collagen groups, collagen alone group, and control group (n = 21 rabbits per group). Histological and biomechanical analyses were performed at 4, 8, and 12 weeks postoperatively to evaluate the effect of aFGF/collagen on tendon–bone interface healing. Results Results of biomechanical tests showed that at both 8 and 12 weeks postoperatively, the elastic modulus and stiffness in both the high and low aFGF/collagen treatment groups were significantly higher than those in the control group and collagen alone group, with that in the high aFGF/collagen concentration group being the highest. Histological analysis showed that at 8 weeks, tightly organized Sharpey-like fibers were observed in both aFGF/collagen groups with new bone growth into the tendon in the high concentration group. At 12 weeks postoperatively, a fibrocartilage transition zone was observed in the bone tunnels in both aFGF/collagen groups, especially in the high aFGF/collagen group. Conclusion Application of the aFGF/collagen composite could enhance early healing at the tendon–bone interface after ACL reconstruction, especially with the use of a high aFGF/collagen concentration.http://link.springer.com/article/10.1186/s13018-018-0984-xaFGFAnterior cruciate ligamentReconstructionTendon–bone healing
spellingShingle Daifeng Lu
Chuandong Yang
Zhitao Zhang
Mochao Xiao
Enhanced tendon–bone healing with acidic fibroblast growth factor delivered in collagen in a rabbit anterior cruciate ligament reconstruction model
Journal of Orthopaedic Surgery and Research
aFGF
Anterior cruciate ligament
Reconstruction
Tendon–bone healing
title Enhanced tendon–bone healing with acidic fibroblast growth factor delivered in collagen in a rabbit anterior cruciate ligament reconstruction model
title_full Enhanced tendon–bone healing with acidic fibroblast growth factor delivered in collagen in a rabbit anterior cruciate ligament reconstruction model
title_fullStr Enhanced tendon–bone healing with acidic fibroblast growth factor delivered in collagen in a rabbit anterior cruciate ligament reconstruction model
title_full_unstemmed Enhanced tendon–bone healing with acidic fibroblast growth factor delivered in collagen in a rabbit anterior cruciate ligament reconstruction model
title_short Enhanced tendon–bone healing with acidic fibroblast growth factor delivered in collagen in a rabbit anterior cruciate ligament reconstruction model
title_sort enhanced tendon bone healing with acidic fibroblast growth factor delivered in collagen in a rabbit anterior cruciate ligament reconstruction model
topic aFGF
Anterior cruciate ligament
Reconstruction
Tendon–bone healing
url http://link.springer.com/article/10.1186/s13018-018-0984-x
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AT chuandongyang enhancedtendonbonehealingwithacidicfibroblastgrowthfactordeliveredincollageninarabbitanteriorcruciateligamentreconstructionmodel
AT zhitaozhang enhancedtendonbonehealingwithacidicfibroblastgrowthfactordeliveredincollageninarabbitanteriorcruciateligamentreconstructionmodel
AT mochaoxiao enhancedtendonbonehealingwithacidicfibroblastgrowthfactordeliveredincollageninarabbitanteriorcruciateligamentreconstructionmodel