Characterization of Natural and Synthetic Sialoglycans Targeting the Hemagglutinin-Neuraminidase of Mumps Virus
The inhibition of surface viral glycoproteins offers great potential to hamper the attachment of viruses to the host cells surface and the spreading of viral infection. Mumps virus (MuV) is the etiological agent of the mumps infectious disease and causes a wide spectrum of mild to severe symptoms du...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-10-01
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| Series: | Frontiers in Chemistry |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fchem.2021.711346/full |
| _version_ | 1818979960953503744 |
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| author | Rosa Ester Forgione Cristina Di Carluccio Francesco Milanesi Francesco Milanesi Marie Kubota Ferran Fabregat Nieto Antonio Molinaro Takao Hashiguchi Oscar Francesconi Roberta Marchetti Alba Silipo |
| author_facet | Rosa Ester Forgione Cristina Di Carluccio Francesco Milanesi Francesco Milanesi Marie Kubota Ferran Fabregat Nieto Antonio Molinaro Takao Hashiguchi Oscar Francesconi Roberta Marchetti Alba Silipo |
| author_sort | Rosa Ester Forgione |
| collection | DOAJ |
| description | The inhibition of surface viral glycoproteins offers great potential to hamper the attachment of viruses to the host cells surface and the spreading of viral infection. Mumps virus (MuV) is the etiological agent of the mumps infectious disease and causes a wide spectrum of mild to severe symptoms due to the inflammation of the salivary glands. Here we focus our attention on the hemagglutinin-neuraminidase (HN) isolated from MuV SBL-1 strain. We describe the molecular features of host sialoglycans recognition by HN protein by means of NMR, fluorescence assays and computational studies. Furthermore, we also describe the synthesis of a N-acetylneuraminic acid-derived thiotrisaccharide targeting the viral protein, and the corresponding 3D-complex. Our results provide the basis to improve the design and synthesis of potent viral hemagglutinin-neuraminidase inhibitors. |
| first_indexed | 2024-12-20T17:07:50Z |
| format | Article |
| id | doaj.art-c0981ab4b67549a8b69c3e25c6de9afc |
| institution | Directory Open Access Journal |
| issn | 2296-2646 |
| language | English |
| last_indexed | 2024-12-20T17:07:50Z |
| publishDate | 2021-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Chemistry |
| spelling | doaj.art-c0981ab4b67549a8b69c3e25c6de9afc2022-12-21T19:32:14ZengFrontiers Media S.A.Frontiers in Chemistry2296-26462021-10-01910.3389/fchem.2021.711346711346Characterization of Natural and Synthetic Sialoglycans Targeting the Hemagglutinin-Neuraminidase of Mumps VirusRosa Ester Forgione0Cristina Di Carluccio1Francesco Milanesi2Francesco Milanesi3Marie Kubota4Ferran Fabregat Nieto5Antonio Molinaro6Takao Hashiguchi7Oscar Francesconi8Roberta Marchetti9Alba Silipo10Department of Chemical Sciences, Complesso Universitario Monte Sant’Angelo, University of Naples Federico II, Naples, ItalyDepartment of Chemical Sciences, Complesso Universitario Monte Sant’Angelo, University of Naples Federico II, Naples, ItalyDepartment of Chemistry “Ugo Schiff” and INSTM, University of Florence Polo Scientifico e Tecnologico, Florence, ItalyMagnetic Resonance Center CERM, Sesto Fiorentino, ItalyDepartment of Virology, Faculty of Medicine, Kyushu University, Fukuoka, JapanDepartment of Chemical Sciences, Complesso Universitario Monte Sant’Angelo, University of Naples Federico II, Naples, ItalyDepartment of Chemical Sciences, Complesso Universitario Monte Sant’Angelo, University of Naples Federico II, Naples, ItalyLaboratory of Medical Virology, Department of Virus Research, Institute for Frontier Life and Medical Sciences, Kyoto University, Kyoto, JapanDepartment of Chemistry “Ugo Schiff” and INSTM, University of Florence Polo Scientifico e Tecnologico, Florence, ItalyDepartment of Chemical Sciences, Complesso Universitario Monte Sant’Angelo, University of Naples Federico II, Naples, ItalyDepartment of Chemical Sciences, Complesso Universitario Monte Sant’Angelo, University of Naples Federico II, Naples, ItalyThe inhibition of surface viral glycoproteins offers great potential to hamper the attachment of viruses to the host cells surface and the spreading of viral infection. Mumps virus (MuV) is the etiological agent of the mumps infectious disease and causes a wide spectrum of mild to severe symptoms due to the inflammation of the salivary glands. Here we focus our attention on the hemagglutinin-neuraminidase (HN) isolated from MuV SBL-1 strain. We describe the molecular features of host sialoglycans recognition by HN protein by means of NMR, fluorescence assays and computational studies. Furthermore, we also describe the synthesis of a N-acetylneuraminic acid-derived thiotrisaccharide targeting the viral protein, and the corresponding 3D-complex. Our results provide the basis to improve the design and synthesis of potent viral hemagglutinin-neuraminidase inhibitors.https://www.frontiersin.org/articles/10.3389/fchem.2021.711346/fullmumpsNMRmolecular recognitioncomputational studiesinhibitors |
| spellingShingle | Rosa Ester Forgione Cristina Di Carluccio Francesco Milanesi Francesco Milanesi Marie Kubota Ferran Fabregat Nieto Antonio Molinaro Takao Hashiguchi Oscar Francesconi Roberta Marchetti Alba Silipo Characterization of Natural and Synthetic Sialoglycans Targeting the Hemagglutinin-Neuraminidase of Mumps Virus Frontiers in Chemistry mumps NMR molecular recognition computational studies inhibitors |
| title | Characterization of Natural and Synthetic Sialoglycans Targeting the Hemagglutinin-Neuraminidase of Mumps Virus |
| title_full | Characterization of Natural and Synthetic Sialoglycans Targeting the Hemagglutinin-Neuraminidase of Mumps Virus |
| title_fullStr | Characterization of Natural and Synthetic Sialoglycans Targeting the Hemagglutinin-Neuraminidase of Mumps Virus |
| title_full_unstemmed | Characterization of Natural and Synthetic Sialoglycans Targeting the Hemagglutinin-Neuraminidase of Mumps Virus |
| title_short | Characterization of Natural and Synthetic Sialoglycans Targeting the Hemagglutinin-Neuraminidase of Mumps Virus |
| title_sort | characterization of natural and synthetic sialoglycans targeting the hemagglutinin neuraminidase of mumps virus |
| topic | mumps NMR molecular recognition computational studies inhibitors |
| url | https://www.frontiersin.org/articles/10.3389/fchem.2021.711346/full |
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