Oxidative damage of DNA induced by the reaction of methylglyoxal with lysine in the presence of ferritin
Methylglyoxal (MG) is an endogenous metabolite which ispresent in increased concentrations in diabetics and reacts withamino acids to form advanced glycation end products. In thisstudy, we investigated whether ferritin enhances DNA cleavageby the reaction of MG with lysine. When plasmid DNA wasincub...
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| Format: | Article |
| Language: | English |
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Korean Society for Biochemistry and Molecular Biology
2013-04-01
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| Series: | BMB Reports |
| Subjects: | |
| Online Access: | http://bmbreports.org/jbmb/pdf.php?data=MTMwOTI3MTBAcGRmX3JhaW50cmFjZV9sZWV5c0AlNUI0Ni00JTVEMTMwNDI2MjA0NF8lMjgyMjUtMjI5JTI5Qk1CXzEyLTIyNS5wZGY= |
| _version_ | 1819230917917409280 |
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| author | Sung Ho An Jung Hoon Kang |
| author_facet | Sung Ho An Jung Hoon Kang |
| author_sort | Sung Ho An |
| collection | DOAJ |
| description | Methylglyoxal (MG) is an endogenous metabolite which ispresent in increased concentrations in diabetics and reacts withamino acids to form advanced glycation end products. In thisstudy, we investigated whether ferritin enhances DNA cleavageby the reaction of MG with lysine. When plasmid DNA wasincubated with MG and lysine in the presence of ferritin, DNAstrand breakage was increased in a dose-dependent manner.The ferritin/MG/lysine system-mediated DNA cleavage wassignificantly inhibited by reactive oxygen species (ROS)scavengers. These results indicated that ROS might participatein the ferritin/MG/lysine system-mediated DNA cleavage.Incubation of ferritin with MG and lysine resulted in atime-dependent release of iron ions from the protein molecules.Our data suggest that DNA cleavage caused by theferritin/MG/lysine system via the generation of ROS by theFenton-like reaction of free iron ions released from oxidativelydamaged ferritin. [BMB Reports 2013; 46(4): 225-229] |
| first_indexed | 2024-12-23T11:36:42Z |
| format | Article |
| id | doaj.art-c09cea8aa8a440df943ed9b81e9cfc74 |
| institution | Directory Open Access Journal |
| issn | 1976-6696 1976-670X |
| language | English |
| last_indexed | 2024-12-23T11:36:42Z |
| publishDate | 2013-04-01 |
| publisher | Korean Society for Biochemistry and Molecular Biology |
| record_format | Article |
| series | BMB Reports |
| spelling | doaj.art-c09cea8aa8a440df943ed9b81e9cfc742022-12-21T17:48:38ZengKorean Society for Biochemistry and Molecular BiologyBMB Reports1976-66961976-670X2013-04-01464225229http://dx.doi.org/10.5483/BMBRep.2013.46.4.225Oxidative damage of DNA induced by the reaction of methylglyoxal with lysine in the presence of ferritinSung Ho AnJung Hoon KangMethylglyoxal (MG) is an endogenous metabolite which ispresent in increased concentrations in diabetics and reacts withamino acids to form advanced glycation end products. In thisstudy, we investigated whether ferritin enhances DNA cleavageby the reaction of MG with lysine. When plasmid DNA wasincubated with MG and lysine in the presence of ferritin, DNAstrand breakage was increased in a dose-dependent manner.The ferritin/MG/lysine system-mediated DNA cleavage wassignificantly inhibited by reactive oxygen species (ROS)scavengers. These results indicated that ROS might participatein the ferritin/MG/lysine system-mediated DNA cleavage.Incubation of ferritin with MG and lysine resulted in atime-dependent release of iron ions from the protein molecules.Our data suggest that DNA cleavage caused by theferritin/MG/lysine system via the generation of ROS by theFenton-like reaction of free iron ions released from oxidativelydamaged ferritin. [BMB Reports 2013; 46(4): 225-229]http://bmbreports.org/jbmb/pdf.php?data=MTMwOTI3MTBAcGRmX3JhaW50cmFjZV9sZWV5c0AlNUI0Ni00JTVEMTMwNDI2MjA0NF8lMjgyMjUtMjI5JTI5Qk1CXzEyLTIyNS5wZGY=DNAFenton reactionFerritinMethylglyoxal |
| spellingShingle | Sung Ho An Jung Hoon Kang Oxidative damage of DNA induced by the reaction of methylglyoxal with lysine in the presence of ferritin BMB Reports DNA Fenton reaction Ferritin Methylglyoxal |
| title | Oxidative damage of DNA induced by the reaction of methylglyoxal with lysine in the presence of ferritin |
| title_full | Oxidative damage of DNA induced by the reaction of methylglyoxal with lysine in the presence of ferritin |
| title_fullStr | Oxidative damage of DNA induced by the reaction of methylglyoxal with lysine in the presence of ferritin |
| title_full_unstemmed | Oxidative damage of DNA induced by the reaction of methylglyoxal with lysine in the presence of ferritin |
| title_short | Oxidative damage of DNA induced by the reaction of methylglyoxal with lysine in the presence of ferritin |
| title_sort | oxidative damage of dna induced by the reaction of methylglyoxal with lysine in the presence of ferritin |
| topic | DNA Fenton reaction Ferritin Methylglyoxal |
| url | http://bmbreports.org/jbmb/pdf.php?data=MTMwOTI3MTBAcGRmX3JhaW50cmFjZV9sZWV5c0AlNUI0Ni00JTVEMTMwNDI2MjA0NF8lMjgyMjUtMjI5JTI5Qk1CXzEyLTIyNS5wZGY= |
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