Heterologous ChAdOx1-BNT162b2 vaccination in Korean cohort induces robust immune and antibody responses that includes Omicron
Summary: Heterologous ChAdOx1-BNT162b2 vaccination induces a stronger immune response than BNT162b2-BNT162b2. Here, we investigated the molecular transcriptome, germline allelic variants of immunoglobulin loci, and anti-Omicron antibody levels in 46 office and lab workers from the Republic of Korea...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-06-01
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| Series: | iScience |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004222007441 |
| _version_ | 1811243273437577216 |
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| author | Hye Kyung Lee Jinyoung Go Heungsup Sung Seong Who Kim Mary Walter Ludwig Knabl Priscilla A. Furth Lothar Hennighausen Jin Won Huh |
| author_facet | Hye Kyung Lee Jinyoung Go Heungsup Sung Seong Who Kim Mary Walter Ludwig Knabl Priscilla A. Furth Lothar Hennighausen Jin Won Huh |
| author_sort | Hye Kyung Lee |
| collection | DOAJ |
| description | Summary: Heterologous ChAdOx1-BNT162b2 vaccination induces a stronger immune response than BNT162b2-BNT162b2. Here, we investigated the molecular transcriptome, germline allelic variants of immunoglobulin loci, and anti-Omicron antibody levels in 46 office and lab workers from the Republic of Korea following ChAdOx1-BNT162b2 vaccination. Anti-spike-specific IgG antibody levels against the ancestral SARS-CoV-2 strain increased from 70 AU/ml to 14,000 AU/ml to 142,000 AU/ml one, three and seven days following the second vaccination. Titers against VOC, including Omicron, were two-fold to three-fold lower, yet higher than those measured following BNT162b2-BNT162b2 vaccination. RNA-seq of peripheral immune cells demonstrated activation of interferon pathways with increased IGHV clonal transcripts encoding neutralizing antibodies. scRNA-seq revealed enriched B cell and CD4+ T cell responses in both ChAdOx1-BNT162b2 and BNT162b2-BNT162b2 recipients, but a stronger clonal expansion of memory B cells with ChAdOx1-BNT162b2. In summary, heterologous ChAdOx1-BNT162b2 provides an innate and adaptive immune response that exceeds homologous BNT162b2 vaccination. |
| first_indexed | 2024-04-12T14:04:33Z |
| format | Article |
| id | doaj.art-c09d81978e194d93955b66f1ed5bb988 |
| institution | Directory Open Access Journal |
| issn | 2589-0042 |
| language | English |
| last_indexed | 2024-04-12T14:04:33Z |
| publishDate | 2022-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj.art-c09d81978e194d93955b66f1ed5bb9882022-12-22T03:30:07ZengElsevieriScience2589-00422022-06-01256104473Heterologous ChAdOx1-BNT162b2 vaccination in Korean cohort induces robust immune and antibody responses that includes OmicronHye Kyung Lee0Jinyoung Go1Heungsup Sung2Seong Who Kim3Mary Walter4Ludwig Knabl5Priscilla A. Furth6Lothar Hennighausen7Jin Won Huh8National Institute of Diabetes, Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA; Corresponding authorDepartment of Biochemistry and Molecular Biology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Republic of KoreaDepartment of Laboratory Medicine, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Republic of KoreaDepartment of Biochemistry and Molecular Biology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Republic of KoreaClinical Core, National Institute of Diabetes, Digestive and Kidney Diseases, Bethesda, MD 20892, USATyrolPath Obrist Brunhuber GmbH, Zams, AustriaDepartments of Oncology & Medicine, Georgetown University, Washington, DC, USA; Corresponding authorNational Institute of Diabetes, Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA; Corresponding authorDepartment of Pulmonary and Critical Care Medicine, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Republic of Korea; Corresponding authorSummary: Heterologous ChAdOx1-BNT162b2 vaccination induces a stronger immune response than BNT162b2-BNT162b2. Here, we investigated the molecular transcriptome, germline allelic variants of immunoglobulin loci, and anti-Omicron antibody levels in 46 office and lab workers from the Republic of Korea following ChAdOx1-BNT162b2 vaccination. Anti-spike-specific IgG antibody levels against the ancestral SARS-CoV-2 strain increased from 70 AU/ml to 14,000 AU/ml to 142,000 AU/ml one, three and seven days following the second vaccination. Titers against VOC, including Omicron, were two-fold to three-fold lower, yet higher than those measured following BNT162b2-BNT162b2 vaccination. RNA-seq of peripheral immune cells demonstrated activation of interferon pathways with increased IGHV clonal transcripts encoding neutralizing antibodies. scRNA-seq revealed enriched B cell and CD4+ T cell responses in both ChAdOx1-BNT162b2 and BNT162b2-BNT162b2 recipients, but a stronger clonal expansion of memory B cells with ChAdOx1-BNT162b2. In summary, heterologous ChAdOx1-BNT162b2 provides an innate and adaptive immune response that exceeds homologous BNT162b2 vaccination.http://www.sciencedirect.com/science/article/pii/S2589004222007441Health sciencesImmunologyImmune response |
| spellingShingle | Hye Kyung Lee Jinyoung Go Heungsup Sung Seong Who Kim Mary Walter Ludwig Knabl Priscilla A. Furth Lothar Hennighausen Jin Won Huh Heterologous ChAdOx1-BNT162b2 vaccination in Korean cohort induces robust immune and antibody responses that includes Omicron iScience Health sciences Immunology Immune response |
| title | Heterologous ChAdOx1-BNT162b2 vaccination in Korean cohort induces robust immune and antibody responses that includes Omicron |
| title_full | Heterologous ChAdOx1-BNT162b2 vaccination in Korean cohort induces robust immune and antibody responses that includes Omicron |
| title_fullStr | Heterologous ChAdOx1-BNT162b2 vaccination in Korean cohort induces robust immune and antibody responses that includes Omicron |
| title_full_unstemmed | Heterologous ChAdOx1-BNT162b2 vaccination in Korean cohort induces robust immune and antibody responses that includes Omicron |
| title_short | Heterologous ChAdOx1-BNT162b2 vaccination in Korean cohort induces robust immune and antibody responses that includes Omicron |
| title_sort | heterologous chadox1 bnt162b2 vaccination in korean cohort induces robust immune and antibody responses that includes omicron |
| topic | Health sciences Immunology Immune response |
| url | http://www.sciencedirect.com/science/article/pii/S2589004222007441 |
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