溶剂法提取微拟球藻脂质的工艺优化及其脂质组学分析Optimization of extraction of Nannochloropsis sp. lipid with solvent and its lipidomics analysis

溶剂法提取微拟球藻脂质的工艺优化及其脂质组学分析Optimization of extraction of Nannochloropsis sp. lipid with solvent and its lipidomics analysis

研究了溶剂法提取微拟球藻脂质的工艺，对比了氯仿-甲醇（体积比1∶ 1）、氯仿、异丙醇、石油醚、正己烷-乙醇（体积比1∶ 1）、正己烷、乙醇7种溶剂对微拟球藻脂质得率和二十碳五烯酸（EPA）含量的影响，确定乙醇作为提取溶剂。采用单因素试验研究了乙醇体积分数、料液比、提取温度和提取时间对微拟球藻脂质提取的影响，并通过正交试验对工艺条件进行优化，最后采用UPLC-Q-TOF-MS对所得微拟球藻脂质进行脂质组学分析。结果表明，微拟球藻脂质最佳提取工艺条件为乙醇体积分数80%、料液比1∶ 17.5、提取温度65 ℃、提取时间2 h。在最佳工艺条件下，微拟球藻脂质得率为（1587±0.21）%，EPA含...

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Bibliographic Details
Main Author:	张美兰1，赵康宇1，张立伟1,2，何东平1,2，郑竟成1,2 ZHANG Meilan1, ZHAO Kangyu1, ZHANG Liwei1,2, HE Dongping1,2， ZHENG Jingcheng1,2
Format:	Article
Language:	English
Published:	中粮工科（西安）国际工程有限公司 2022-05-01
Series:	Zhongguo youzhi
Subjects:	微拟球藻；脂质；二十碳五烯酸；乙醇提取；脂质组学 nannochloropsis sp. ; lipid;eicosapentaenoic acid; ethanol extraction; lipidomics
Online Access:	http://tg.chinaoils.cn/ch/reader/create_pdf.aspx?file_no=20220502&year_id=2022&quarter_id=5&falg=1

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